Values CatLog read from this paper

Evolution of enzymatic activities in the enolase superfamily: crystallographic and mutagenesis studies of the reaction catalyzed by D-glucarate dehydratase from Escherichia coli

PMID 10769114doi:10.1021/bi992782iOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 19 records (37 values) from this paper: D-glucarate dehydratase (GlucD) from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 4.2.1.40

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
D-glucarate dehydratase (GlucD)	Escherichia coli	D-glucarateTable 4	35	—	580	pH 7.5, 22 °C	Open record: D-glucarate dehydratase (GlucD), D-glucarate
D-glucarate dehydratase (GlucD)	Escherichia coli	D-glucaratein the text	—	0.16	—	pH 7.5, 22 °C	Open record: D-glucarate dehydratase (GlucD), D-glucarate
D-glucarate dehydratase (GlucD)	Escherichia coli	L-idarate	34	—	210	pH 7.5, 22 °C	Open record: D-glucarate dehydratase (GlucD), L-idarate
D-glucarate dehydratase (GlucD)variant D366A	Escherichia coli	D-glucarate	1	—	0.51	pH 7.5, 22 °C	Open record: D-glucarate dehydratase (GlucD) variant D366A, D-glucarate
D-glucarate dehydratase (GlucD)variant D366A	Escherichia coli	L-idarate	1.5	—	0.32	pH 7.5, 22 °C	Open record: D-glucarate dehydratase (GlucD) variant D366A, L-idarate
D-glucarate dehydratase (GlucD)variant D366N	Escherichia coli	D-glucarate	1.8	—	3.1	pH 7.5, 22 °C	Open record: D-glucarate dehydratase (GlucD) variant D366N, D-glucarate
D-glucarate dehydratase (GlucD)variant D366N	Escherichia coli	L-idarate	8	—	0.9	pH 7.5, 22 °C	Open record: D-glucarate dehydratase (GlucD) variant D366N, L-idarate
D-glucarate dehydratase (GlucD)variant H339A	Escherichia coli	D-glucarate	0.016	—	1.4 × 10⁻⁵	pH 7.5, 22 °C	Open record: D-glucarate dehydratase (GlucD) variant H339A, D-glucarate
D-glucarate dehydratase (GlucD)variant H339A	Escherichia coli	L-idarate	0.0051	—	0.075	pH 7.5, 22 °C	Open record: D-glucarate dehydratase (GlucD) variant H339A, L-idarate
D-glucarate dehydratase (GlucD)variant H339N	Escherichia coli	D-glucarate	3.3 × 10⁻⁴	—	0.0072	pH 7.5, 22 °C	Open record: D-glucarate dehydratase (GlucD) variant H339N, D-glucarate
D-glucarate dehydratase (GlucD)variant H339N	Escherichia coli	L-idarate	2.5 × 10⁻⁴	—	0.0029	pH 7.5, 22 °C	Open record: D-glucarate dehydratase (GlucD) variant H339N, L-idarate
D-glucarate dehydratase (GlucD)variant H339Q	Escherichia coli	D-glucarate	0.02	—	0.42	pH 7.5, 22 °C	Open record: D-glucarate dehydratase (GlucD) variant H339Q, D-glucarate
D-glucarate dehydratase (GlucD)variant H339Q	Escherichia coli	L-idarate	0.02	—	0.047	pH 7.5, 22 °C	Open record: D-glucarate dehydratase (GlucD) variant H339Q, L-idarate
D-glucarate dehydratase (GlucD)variant K207Q	Escherichia coli	D-glucarate	0.005	—	0.11	pH 7.5, 22 °C	Open record: D-glucarate dehydratase (GlucD) variant K207Q, D-glucarate
D-glucarate dehydratase (GlucD)variant K207Q	Escherichia coli	L-idarate	0.0015	—	0.017	pH 7.5, 22 °C	Open record: D-glucarate dehydratase (GlucD) variant K207Q, L-idarate
D-glucarate dehydratase (GlucD)variant K207R	Escherichia coli	D-glucarate	0.001	—	0.01	pH 7.5, 22 °C	Open record: D-glucarate dehydratase (GlucD) variant K207R, D-glucarate
D-glucarate dehydratase (GlucD)variant K207R	Escherichia coli	L-idarate	0.0015	—	0.0065	pH 7.5, 22 °C	Open record: D-glucarate dehydratase (GlucD) variant K207R, L-idarate
D-glucarate dehydratase (GlucD)variant Y150F	Escherichia coli	D-glucarate	0.19	—	2.2	pH 7.5, 22 °C	Open record: D-glucarate dehydratase (GlucD) variant Y150F, D-glucarate
D-glucarate dehydratase (GlucD)variant Y150F	Escherichia coli	L-idarate	0.08	—	0.38	pH 7.5, 22 °C	Open record: D-glucarate dehydratase (GlucD) variant Y150F, L-idarate