Values CatLog read from this paper
Comparative structural analysis and substrate specificity engineering of the hyperthermostable beta-glucosidase CelB from Pyrococcus furiosus
PMID 10819960doi:10.1021/bi992463rOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 16 records (48 values) from this paper: CelB from Pyrococcus furiosus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| CelB | Pyrococcus furiosus | o-nitrophenyl-beta-D-galactopyranoside | 2.05 × 10³ | 2.3 | 899 | pH 5, 90 °C | Open record: CelB, o-nitrophenyl-beta-D-galactopyranoside |
| CelB | Pyrococcus furiosus | o-nitrophenyl-beta-D-galactopyranoside-6-phosphate | 49 | 31 | 1.6 | pH 4, 90 °C | Open record: CelB, o-nitrophenyl-beta-D-galactopyranoside-6-phosphate |
| CelB | Pyrococcus furiosus | o-nitrophenyl-beta-D-glucopyranoside | 860 | 0.28 | 4048 | pH 5, 90 °C | Open record: CelB, o-nitrophenyl-beta-D-glucopyranoside |
| CelBvariant E417S | Pyrococcus furiosus | o-nitrophenyl-beta-D-galactopyranoside | 1.06 × 10³ | 42 | 25.3 | pH 4.5, 90 °C | Open record: CelB variant E417S, o-nitrophenyl-beta-D-galactopyranoside |
| CelBvariant E417S | Pyrococcus furiosus | o-nitrophenyl-beta-D-galactopyranoside-6-phosphate | 171 | 22 | 7.7 | pH 5, 90 °C | Open record: CelB variant E417S, o-nitrophenyl-beta-D-galactopyranoside-6-phosphate |
| CelBvariant E417S | Pyrococcus furiosus | o-nitrophenyl-beta-D-glucopyranoside | 3.3 × 10³ | 29 | 113 | pH 4.5, 90 °C | Open record: CelB variant E417S, o-nitrophenyl-beta-D-glucopyranoside |
| CelBvariant E417S/M424K/F426Y | Pyrococcus furiosus | o-nitrophenyl-beta-D-galactopyranoside | 175 | 40 | 4.4 | pH 5, 90 °C | Open record: CelB variant E417S/M424K/F426Y, o-nitrophenyl-beta-D-galactopyranoside |
| CelBvariant E417S/M424K/F426Y | Pyrococcus furiosus | o-nitrophenyl-beta-D-galactopyranoside-6-phosphate | 69 | 16 | 4.2 | pH 6, 90 °C | Open record: CelB variant E417S/M424K/F426Y, o-nitrophenyl-beta-D-galactopyranoside-6-phosphate |
| CelBvariant E417S/M424K/F426Y | Pyrococcus furiosus | o-nitrophenyl-beta-D-glucopyranoside | 777 | 59 | 13 | pH 5, 90 °C | Open record: CelB variant E417S/M424K/F426Y, o-nitrophenyl-beta-D-glucopyranoside |
| CelBvariant F426Y | Pyrococcus furiosus | o-nitrophenyl-beta-D-galactopyranoside | 892 | 0.89 | 1 × 10³ | pH 5, 90 °C | Open record: CelB variant F426Y, o-nitrophenyl-beta-D-galactopyranoside |
| CelBvariant F426Y | Pyrococcus furiosus | o-nitrophenyl-beta-D-galactopyranoside-6-phosphate | 26 | 38 | 0.7 | pH 4, 90 °C | Open record: CelB variant F426Y, o-nitrophenyl-beta-D-galactopyranoside-6-phosphate |
| CelBvariant F426Y | Pyrococcus furiosus | o-nitrophenyl-beta-D-glucopyranoside | 840 | 0.57 | 1484 | pH 5, 90 °C | Open record: CelB variant F426Y, o-nitrophenyl-beta-D-glucopyranoside |
| CelBvariant M424K | Pyrococcus furiosus | o-nitrophenyl-beta-D-galactopyranoside | 1.88 × 10³ | 4.8 | 396 | pH 5.75, 90 °C | Open record: CelB variant M424K, o-nitrophenyl-beta-D-galactopyranoside |
| CelBvariant M424K | Pyrococcus furiosus | o-nitrophenyl-beta-D-glucopyranoside | 772 | 0.51 | 1.51 × 10³ | pH 6, 90 °C | Open record: CelB variant M424K, o-nitrophenyl-beta-D-glucopyranoside |
| CelBvariant M424K/F426Y | Pyrococcus furiosus | o-nitrophenyl-beta-D-galactopyranoside | 969 | 3 | 325 | pH 6.25, 90 °C | Open record: CelB variant M424K/F426Y, o-nitrophenyl-beta-D-galactopyranoside |
| CelBvariant M424K/F426Y | Pyrococcus furiosus | o-nitrophenyl-beta-D-glucopyranoside | 1.4 × 10³ | 3 | 422 | pH 6, 90 °C | Open record: CelB variant M424K/F426Y, o-nitrophenyl-beta-D-glucopyranoside |