Values CatLog read from this paper
Roles of two conserved amino acid residues in the active site of galactose-1-phosphate uridylyltransferase: an essential serine and a nonessential cysteine
PMID 10820011doi:10.1021/bi992594sOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 10 records (15 values) from this paper: galactose-1-phosphate uridylyltransferase from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 5 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| galactose-1-phosphate uridylyltransferasevariant C160A | Escherichia coli | galactose-1-phosphate | — | 0.125 | — | pH 8.5, 27 °C | Open record: galactose-1-phosphate uridylyltransferase variant C160A, galactose-1-phosphate |
| galactose-1-phosphate uridylyltransferasevariant C160A | Escherichia coli | glucose-1-phosphate | — | 0.138 | — | pH 8.5, 27 °C | Open record: galactose-1-phosphate uridylyltransferase variant C160A, glucose-1-phosphate |
| galactose-1-phosphate uridylyltransferasevariant C160A | Escherichia coli | uridine 5'-diphosphate galactose | 55 | 0.078 | 710 calc. | pH 8.5, 27 °C | Open record: galactose-1-phosphate uridylyltransferase variant C160A, uridine 5'-diphosphate galactose |
| galactose-1-phosphate uridylyltransferasevariant C160A | Escherichia coli | uridine 5'-diphosphate glucose | 265 | 0.082 | 3.2 × 10³ calc. | pH 8.5, 27 °C | Open record: galactose-1-phosphate uridylyltransferase variant C160A, uridine 5'-diphosphate glucose |
| galactose-1-phosphate uridylyltransferasevariant S161A | Escherichia coli | galactose-1-phosphate | — | 0.223 | — | pH 8.5, 27 °C | Open record: galactose-1-phosphate uridylyltransferase variant S161A, galactose-1-phosphate |
| galactose-1-phosphate uridylyltransferasevariant S161A | Escherichia coli | galactose-1-phosphate | — | 0.57 | — | 4 °C | Open record: galactose-1-phosphate uridylyltransferase variant S161A, galactose-1-phosphate |
| galactose-1-phosphate uridylyltransferasevariant S161A | Escherichia coli | glucose-1-phosphate | — | 0.195 | — | pH 8.5, 27 °C | Open record: galactose-1-phosphate uridylyltransferase variant S161A, glucose-1-phosphate |
| galactose-1-phosphate uridylyltransferasevariant S161A | Escherichia coli | uridine 5'-diphosphate galactose | 0.096 | 0.062 | 1.5 calc. | pH 8.5, 27 °C | Open record: galactose-1-phosphate uridylyltransferase variant S161A, uridine 5'-diphosphate galactose |
| galactose-1-phosphate uridylyltransferasevariant S161A | Escherichia coli | uridine 5'-diphosphate glucose | 0.054 | 0.072 | 0.75 calc. | pH 8.5, 27 °C | Open record: galactose-1-phosphate uridylyltransferase variant S161A, uridine 5'-diphosphate glucose |
| galactose-1-phosphate uridylyltransferasevariant S161A | Escherichia coli | uridine 5'-diphosphate glucose | 9 × 10⁻⁴ | 0.12 | 0.0075 calc. | 4 °C | Open record: galactose-1-phosphate uridylyltransferase variant S161A, uridine 5'-diphosphate glucose |