CatLogenzyme kinetics catalog

Values CatLog read from this paper

Catalytic action of fuculose 1-phosphate aldolase (class II) as derived from structure-directed mutagenesis

PMID 10821675doi:10.1021/bi9927686Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 15 records (30 values) from this paper: L-fuculose 1-phosphate aldolase (FucA) from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 15 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 4.1.2.17

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
L-fuculose 1-phosphate aldolase (FucA)Escherichia coliL-fuculose 1-phosphate19.32.28.8 calc.pH 7.5, 37 °COpen
L-fuculose 1-phosphate aldolase (FucA)variant Del(207-215)Escherichia coliL-fuculose 1-phosphate1.5870.017 calc.pH 7.5, 37 °COpen
L-fuculose 1-phosphate aldolase (FucA)variant Del(211-215)Escherichia coliL-fuculose 1-phosphate7210.33 calc.pH 7.5, 37 °COpen
L-fuculose 1-phosphate aldolase (FucA)variant E214AEscherichia coliL-fuculose 1-phosphate18.582.3 calc.pH 7.5, 37 °COpen
L-fuculose 1-phosphate aldolase (FucA)variant E214DEscherichia coliL-fuculose 1-phosphate19.536.5 calc.pH 7.5, 37 °COpen
L-fuculose 1-phosphate aldolase (FucA)variant E214LEscherichia coliL-fuculose 1-phosphate12.8740.17 calc.pH 7.5, 37 °COpen
L-fuculose 1-phosphate aldolase (FucA)variant E214QEscherichia coliL-fuculose 1-phosphate20.563.4 calc.pH 7.5, 37 °COpen
L-fuculose 1-phosphate aldolase (FucA)variant E215AEscherichia coliL-fuculose 1-phosphate21.53.16.9 calc.pH 7.5, 37 °COpen
L-fuculose 1-phosphate aldolase (FucA)variant F131AEscherichia coliL-fuculose 1-phosphate0.6220.027 calc.pH 7.5, 37 °COpen
L-fuculose 1-phosphate aldolase (FucA)variant F206WEscherichia coliL-fuculose 1-phosphate8.3110.75 calc.pH 7.5, 37 °COpen
L-fuculose 1-phosphate aldolase (FucA)variant K207AEscherichia coliL-fuculose 1-phosphate19.572.8 calc.pH 7.5, 37 °COpen
L-fuculose 1-phosphate aldolase (FucA)variant R212AEscherichia coliL-fuculose 1-phosphate121.86.7 calc.pH 7.5, 37 °COpen
L-fuculose 1-phosphate aldolase (FucA)variant T26AEscherichia coliL-fuculose 1-phosphate460.67 calc.pH 7.5, 37 °COpen
L-fuculose 1-phosphate aldolase (FucA)variant Y113FEscherichia coliL-fuculose 1-phosphate0.40.50.80 calc.pH 7.5, 37 °COpen
L-fuculose 1-phosphate aldolase (FucA)variant Y209FEscherichia coliL-fuculose 1-phosphate1.34.40.30 calc.pH 7.5, 37 °COpen