Values CatLog read from this paper
Role of arginine 285 in the active site of Rhodotorula gracilis D-amino acid oxidase. A site-directed mutagenesis study
PMID 10821840doi:10.1074/jbc.m908193199Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 4 records (6 values) from this paper: D-amino acid oxidase from Rhodotorula gracilis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 2 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| D-amino acid oxidasevariant R285A | Rhodotorula gracilis | D-alanine | 0.05 | 310 | 1.6 × 10⁻⁴ calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase variant R285A, D-alanine |
| D-amino acid oxidasevariant R285A | Rhodotorula gracilis | oxygen | — | 0.08 | — | pH 8.5, 25 °C | Open record: D-amino acid oxidase variant R285A, oxygen |
| D-amino acid oxidasevariant R285K | Rhodotorula gracilis | D-alanine | 0.8 | 800 | 0.0010 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase variant R285K, D-alanine |
| D-amino acid oxidasevariant R285K | Rhodotorula gracilis | oxygen | — | 0.06 | — | pH 8.5, 25 °C | Open record: D-amino acid oxidase variant R285K, oxygen |