Values CatLog read from this paper

Penicillopepsin-JT2, a recombinant enzyme from Penicillium janthinellum and the contribution of a hydrogen bond in subsite S3 to k(cat)

PMID 10850809doi:10.1110/ps.9.5.991Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 48 records (96 values) from this paper: penicillopepsin-JT2 from Penicillium janthinellum.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 48 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 3.4.23.20

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
penicillopepsin-JT2	Penicillium janthinellum	N-acetyl-alanyl-alanyl-alanyl-lysyl-p-nitrophenylalanyl-alanyl-alaninamide (Nph = p-nitrophenylalanyl)	34	0.46	74 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2, N-acetyl-alanyl-alanyl-alanyl-lysyl-p-nitrophenylalanyl-alanyl-alaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2	Penicillium janthinellum	N-acetyl-alanyl-alanyl-lysyl-p-nitrophenylalaninamide (Nph = p-nitrophenylalanyl)	0.36	0.5	0.72 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2, N-acetyl-alanyl-alanyl-lysyl-p-nitrophenylalaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2	Penicillium janthinellum	N-acetyl-alanyl-alanyl-lysyl-p-nitrophenylalanyl-alaninamide (Nph = p-nitrophenylalanyl)	15	0.35	43 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2, N-acetyl-alanyl-alanyl-lysyl-p-nitrophenylalanyl-alaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2	Penicillium janthinellum	N-acetyl-alanyl-alanyl-lysyl-p-nitrophenylalanyl-alanyl-alaninamide (Nph = p-nitrophenylalanyl)	40	0.32	120 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2, N-acetyl-alanyl-alanyl-lysyl-p-nitrophenylalanyl-alanyl-alaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2	Penicillium janthinellum	N-acetyl-alanyl-lysyl-p-nitrophenylalaninamide (Nph = p-nitrophenylalanyl)	0.003	0.4	0.0075 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2, N-acetyl-alanyl-lysyl-p-nitrophenylalaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2	Penicillium janthinellum	N-acetyl-alanyl-lysyl-p-nitrophenylalanyl-alaninamide (Nph = p-nitrophenylalanyl)	0.6	0.5	1.2 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2, N-acetyl-alanyl-lysyl-p-nitrophenylalanyl-alaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2	Penicillium janthinellum	N-acetyl-lysyl-p-nitrophenylalaninamide (Nph = p-nitrophenylalanyl)	0.002	0.4	0.0050 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2, N-acetyl-lysyl-p-nitrophenylalaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2	Penicillium janthinellum	N-acetyl-lysyl-p-nitrophenylalanyl-alaninamide (Nph = p-nitrophenylalanyl)	0.4	0.59	0.68 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2, N-acetyl-lysyl-p-nitrophenylalanyl-alaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2	Penicillium janthinellum	N-acetyl-lysyl-p-nitrophenylalanyl-alanyl-alaninamide (Nph = p-nitrophenylalanyl)	0.29	0.21	1.4 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2, N-acetyl-lysyl-p-nitrophenylalanyl-alanyl-alaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2variant T219A	Penicillium janthinellum	N-acetyl-alanyl-alanyl-alanyl-lysyl-p-nitrophenylalanyl-alanyl-alaninamide (Nph = p-nitrophenylalanyl)	0.36	0.68	0.53 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2 variant T219A, N-acetyl-alanyl-alanyl-alanyl-lysyl-p-nitrophenylalanyl-alanyl-alaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2variant T219A	Penicillium janthinellum	N-acetyl-alanyl-alanyl-lysyl-p-nitrophenylalaninamide (Nph = p-nitrophenylalanyl)	0.004	0.8	0.0050 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2 variant T219A, N-acetyl-alanyl-alanyl-lysyl-p-nitrophenylalaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2variant T219A	Penicillium janthinellum	N-acetyl-alanyl-alanyl-lysyl-p-nitrophenylalanyl-alaninamide (Nph = p-nitrophenylalanyl)	0.09	0.71	0.13 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2 variant T219A, N-acetyl-alanyl-alanyl-lysyl-p-nitrophenylalanyl-alaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2variant T219A	Penicillium janthinellum	N-acetyl-alanyl-alanyl-lysyl-p-nitrophenylalanyl-alanyl-alaninamide (Nph = p-nitrophenylalanyl)	0.45	0.59	0.76 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2 variant T219A, N-acetyl-alanyl-alanyl-lysyl-p-nitrophenylalanyl-alanyl-alaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2variant T219A	Penicillium janthinellum	N-acetyl-alanyl-lysyl-p-nitrophenylalaninamide (Nph = p-nitrophenylalanyl)	0.005	0.75	0.0067 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2 variant T219A, N-acetyl-alanyl-lysyl-p-nitrophenylalaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2variant T219A	Penicillium janthinellum	N-acetyl-alanyl-lysyl-p-nitrophenylalanyl-alaninamide (Nph = p-nitrophenylalanyl)	0.046	0.6	0.077 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2 variant T219A, N-acetyl-alanyl-lysyl-p-nitrophenylalanyl-alaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2variant T219A	Penicillium janthinellum	N-acetyl-alanyl-lysyl-p-nitrophenylalanyl-alanyl-alaninamide (Nph = p-nitrophenylalanyl)	0.3	0.6	0.50 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2 variant T219A, N-acetyl-alanyl-lysyl-p-nitrophenylalanyl-alanyl-alaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2variant T219A	Penicillium janthinellum	N-acetyl-lysyl-p-nitrophenylalaninamide (Nph = p-nitrophenylalanyl)	0.002	0.65	0.0031 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2 variant T219A, N-acetyl-lysyl-p-nitrophenylalaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2variant T219A	Penicillium janthinellum	N-acetyl-lysyl-p-nitrophenylalanyl-alaninamide (Nph = p-nitrophenylalanyl)	0.042	0.83	0.051 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2 variant T219A, N-acetyl-lysyl-p-nitrophenylalanyl-alaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2variant T219A	Penicillium janthinellum	N-acetyl-lysyl-p-nitrophenylalanyl-alanyl-alaninamide (Nph = p-nitrophenylalanyl)	0.12	0.77	0.16 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2 variant T219A, N-acetyl-lysyl-p-nitrophenylalanyl-alanyl-alaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2variant T219G	Penicillium janthinellum	N-acetyl-alanyl-alanyl-alanyl-lysyl-p-nitrophenylalanyl-alanyl-alaninamide (Nph = p-nitrophenylalanyl)	0.45	0.55	0.82 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2 variant T219G, N-acetyl-alanyl-alanyl-alanyl-lysyl-p-nitrophenylalanyl-alanyl-alaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2variant T219G	Penicillium janthinellum	N-acetyl-alanyl-alanyl-lysyl-p-nitrophenylalaninamide (Nph = p-nitrophenylalanyl)	0.003	0.45	0.0067 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2 variant T219G, N-acetyl-alanyl-alanyl-lysyl-p-nitrophenylalaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2variant T219G	Penicillium janthinellum	N-acetyl-alanyl-alanyl-lysyl-p-nitrophenylalanyl-alaninamide (Nph = p-nitrophenylalanyl)	0.35	0.45	0.78 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2 variant T219G, N-acetyl-alanyl-alanyl-lysyl-p-nitrophenylalanyl-alaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2variant T219G	Penicillium janthinellum	N-acetyl-alanyl-alanyl-lysyl-p-nitrophenylalanyl-alanyl-alaninamide (Nph = p-nitrophenylalanyl)	0.5	0.4	1.2 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2 variant T219G, N-acetyl-alanyl-alanyl-lysyl-p-nitrophenylalanyl-alanyl-alaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2variant T219G	Penicillium janthinellum	N-acetyl-alanyl-lysyl-p-nitrophenylalaninamide (Nph = p-nitrophenylalanyl)	0.003	0.54	0.0056 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2 variant T219G, N-acetyl-alanyl-lysyl-p-nitrophenylalaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2variant T219G	Penicillium janthinellum	N-acetyl-alanyl-lysyl-p-nitrophenylalanyl-alaninamide (Nph = p-nitrophenylalanyl)	0.13	0.83	0.16 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2 variant T219G, N-acetyl-alanyl-lysyl-p-nitrophenylalanyl-alaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2variant T219G	Penicillium janthinellum	N-acetyl-alanyl-lysyl-p-nitrophenylalanyl-alanyl-alaninamide (Nph = p-nitrophenylalanyl)	0.24	0.45	0.53 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2 variant T219G, N-acetyl-alanyl-lysyl-p-nitrophenylalanyl-alanyl-alaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2variant T219G	Penicillium janthinellum	N-acetyl-lysyl-p-nitrophenylalaninamide (Nph = p-nitrophenylalanyl)	0.005	0.48	0.010 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2 variant T219G, N-acetyl-lysyl-p-nitrophenylalaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2variant T219G	Penicillium janthinellum	N-acetyl-lysyl-p-nitrophenylalanyl-alaninamide (Nph = p-nitrophenylalanyl)	0.14	0.74	0.19 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2 variant T219G, N-acetyl-lysyl-p-nitrophenylalanyl-alaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2variant T219G	Penicillium janthinellum	N-acetyl-lysyl-p-nitrophenylalanyl-alanyl-alaninamide (Nph = p-nitrophenylalanyl)	0.43	0.6	0.72 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2 variant T219G, N-acetyl-lysyl-p-nitrophenylalanyl-alanyl-alaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2variant T219S	Penicillium janthinellum	N-acetyl-alanyl-alanyl-alanyl-lysyl-p-nitrophenylalanyl-alanyl-alaninamide (Nph = p-nitrophenylalanyl)	16.1	0.39	41 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2 variant T219S, N-acetyl-alanyl-alanyl-alanyl-lysyl-p-nitrophenylalanyl-alanyl-alaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2variant T219S	Penicillium janthinellum	N-acetyl-alanyl-alanyl-lysyl-p-nitrophenylalaninamide (Nph = p-nitrophenylalanyl)	0.11	0.37	0.30 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2 variant T219S, N-acetyl-alanyl-alanyl-lysyl-p-nitrophenylalaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2variant T219S	Penicillium janthinellum	N-acetyl-alanyl-alanyl-lysyl-p-nitrophenylalanyl-alaninamide (Nph = p-nitrophenylalanyl)	3.8	0.48	7.9 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2 variant T219S, N-acetyl-alanyl-alanyl-lysyl-p-nitrophenylalanyl-alaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2variant T219S	Penicillium janthinellum	N-acetyl-alanyl-alanyl-lysyl-p-nitrophenylalanyl-alanyl-alaninamide (Nph = p-nitrophenylalanyl)	14.9	0.48	31 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2 variant T219S, N-acetyl-alanyl-alanyl-lysyl-p-nitrophenylalanyl-alanyl-alaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2variant T219S	Penicillium janthinellum	N-acetyl-alanyl-lysyl-p-nitrophenylalaninamide (Nph = p-nitrophenylalanyl)	0.003	0.42	0.0071 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2 variant T219S, N-acetyl-alanyl-lysyl-p-nitrophenylalaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2variant T219S	Penicillium janthinellum	N-acetyl-alanyl-lysyl-p-nitrophenylalanyl-alaninamide (Nph = p-nitrophenylalanyl)	0.09	0.3	0.30 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2 variant T219S, N-acetyl-alanyl-lysyl-p-nitrophenylalanyl-alaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2variant T219S	Penicillium janthinellum	N-acetyl-lysyl-p-nitrophenylalaninamide (Nph = p-nitrophenylalanyl)	0.003	0.51	0.0059 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2 variant T219S, N-acetyl-lysyl-p-nitrophenylalaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2variant T219S	Penicillium janthinellum	N-acetyl-lysyl-p-nitrophenylalanyl-alaninamide (Nph = p-nitrophenylalanyl)	0.07	0.5	0.14 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2 variant T219S, N-acetyl-lysyl-p-nitrophenylalanyl-alaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2variant T219S	Penicillium janthinellum	N-acetyl-lysyl-p-nitrophenylalanyl-alanyl-alaninamide (Nph = p-nitrophenylalanyl)	0.33	0.6	0.55 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2 variant T219S, N-acetyl-lysyl-p-nitrophenylalanyl-alanyl-alaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2variant T219V	Penicillium janthinellum	N-acetyl-alanyl-alanyl-alanyl-lysyl-p-nitrophenylalanyl-alanyl-alaninamide (Nph = p-nitrophenylalanyl)	0.35	0.59	0.59 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2 variant T219V, N-acetyl-alanyl-alanyl-alanyl-lysyl-p-nitrophenylalanyl-alanyl-alaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2variant T219V	Penicillium janthinellum	N-acetyl-alanyl-alanyl-lysyl-p-nitrophenylalaninamide (Nph = p-nitrophenylalanyl)	0.0019	0.6	0.0032 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2 variant T219V, N-acetyl-alanyl-alanyl-lysyl-p-nitrophenylalaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2variant T219V	Penicillium janthinellum	N-acetyl-alanyl-alanyl-lysyl-p-nitrophenylalanyl-alaninamide (Nph = p-nitrophenylalanyl)	0.12	0.71	0.17 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2 variant T219V, N-acetyl-alanyl-alanyl-lysyl-p-nitrophenylalanyl-alaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2variant T219V	Penicillium janthinellum	N-acetyl-alanyl-alanyl-lysyl-p-nitrophenylalanyl-alanyl-alaninamide (Nph = p-nitrophenylalanyl)	0.3	0.5	0.60 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2 variant T219V, N-acetyl-alanyl-alanyl-lysyl-p-nitrophenylalanyl-alanyl-alaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2variant T219V	Penicillium janthinellum	N-acetyl-alanyl-lysyl-p-nitrophenylalaninamide (Nph = p-nitrophenylalanyl)	0.0054	0.6	0.0090 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2 variant T219V, N-acetyl-alanyl-lysyl-p-nitrophenylalaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2variant T219V	Penicillium janthinellum	N-acetyl-alanyl-lysyl-p-nitrophenylalanyl-alaninamide (Nph = p-nitrophenylalanyl)	0.23	0.8	0.29 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2 variant T219V, N-acetyl-alanyl-lysyl-p-nitrophenylalanyl-alaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2variant T219V	Penicillium janthinellum	N-acetyl-alanyl-lysyl-p-nitrophenylalanyl-alanyl-alaninamide (Nph = p-nitrophenylalanyl)	0.45	0.45	1.0 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2 variant T219V, N-acetyl-alanyl-lysyl-p-nitrophenylalanyl-alanyl-alaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2variant T219V	Penicillium janthinellum	N-acetyl-lysyl-p-nitrophenylalaninamide (Nph = p-nitrophenylalanyl)	0.0016	0.46	0.0035 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2 variant T219V, N-acetyl-lysyl-p-nitrophenylalaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2variant T219V	Penicillium janthinellum	N-acetyl-lysyl-p-nitrophenylalanyl-alaninamide (Nph = p-nitrophenylalanyl)	0.05	0.57	0.088 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2 variant T219V, N-acetyl-lysyl-p-nitrophenylalanyl-alaninamide (Nph = p-nitrophenylalanyl)
penicillopepsin-JT2variant T219V	Penicillium janthinellum	N-acetyl-lysyl-p-nitrophenylalanyl-alanyl-alaninamide (Nph = p-nitrophenylalanyl)	0.17	0.55	0.31 calc.	pH 5, 25 °C	Open record: penicillopepsin-JT2 variant T219V, N-acetyl-lysyl-p-nitrophenylalanyl-alanyl-alaninamide (Nph = p-nitrophenylalanyl)