Values CatLog read from this paper

Kinetic and structural characterization of urease active site variants

PMID 10913264doi:10.1021/bi000613oOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 10 records (20 values) from this paper: urease from Klebsiella aerogenes.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 10 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 3.5.1.5

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
urease	Klebsiella aerogenes	urea	2.97 × 10³	2.4	1.2 × 10³ calc.	pH 7.75	Open record: urease, urea
ureasevariant D221A	Klebsiella aerogenes	urea	1.7	24	0.071 calc.	pH 5.1	Open record: urease variant D221A, urea
ureasevariant D221N	Klebsiella aerogenes	urea	60	3.6	17 calc.	pH 7.75	Open record: urease variant D221N, urea
ureasevariant H219A	Klebsiella aerogenes	urea	194	2.09 × 10³	0.0928 calc.	pH 7.75	Open record: urease variant H219A, urea
ureasevariant H219N	Klebsiella aerogenes	urea	322	175	1.84 calc.	pH 6.2	Open record: urease variant H219N, urea
ureasevariant H219Q	Klebsiella aerogenes	urea	1.86 × 10³	227	8.19 calc.	pH 6.2	Open record: urease variant H219Q, urea
ureasevariant H320A	Klebsiella aerogenes	urea	0.068	10.9	0.0062 calc.	pH 6.2	Open record: urease variant H320A, urea
ureasevariant H320N	Klebsiella aerogenes	urea	0.06	7.4	0.0081 calc.	pH 6.2	Open record: urease variant H320N, urea
ureasevariant H320Q	Klebsiella aerogenes	urea	0.033	10.6	0.0031 calc.	pH 6.2	Open record: urease variant H320Q, urea
ureasevariant R336Q	Klebsiella aerogenes	urea	0.62	2.7	0.23 calc.	pH 7.75	Open record: urease variant R336Q, urea