Values CatLog read from this paper
Transgalactosylation by thermostable beta-glycosidases from Pyrococcus furiosus and Sulfolobus solfataricus. Binding interactions of nucleophiles with the galactosylated enzyme intermediate make…
PMID 10931188doi:10.1046/j.1432-1327.2000.01562.xOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 8 records (20 values) from this paper: CelB (β-glycosidase from Pyrococcus furiosus), SsβGly (β-glycosidase from Sulfolobus solfataricus) from Pyrococcus furiosus, Sulfolobus solfataricus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| CelB (β-glycosidase from Pyrococcus furiosus) | Pyrococcus furiosus | 2-nitrophenyl beta-D-galactopyranoside | 1 × 10⁴ | 5.3 | 1.9 × 10³ | pH 6.5, 80 °C | Open record: CelB (β-glycosidase from Pyrococcus furiosus), 2-nitrophenyl beta-D-galactopyranoside |
| CelB (β-glycosidase from Pyrococcus furiosus) | Pyrococcus furiosus | 2-nitrophenyl beta-D-glucopyranoside | — | 0.2 | — | Open record: CelB (β-glycosidase from Pyrococcus furiosus), 2-nitrophenyl beta-D-glucopyranoside | |
| CelB (β-glycosidase from Pyrococcus furiosus) | Pyrococcus furiosus | beta-D-galactopyranosyl-(1->4)-D-glucose | 3.8 × 10³ | 186 | 20.4 | pH 6.5, 80 °C | Open record: CelB (β-glycosidase from Pyrococcus furiosus), beta-D-galactopyranosyl-(1->4)-D-glucose |
| CelB (β-glycosidase from Pyrococcus furiosus) | Pyrococcus furiosus | methyl beta-D-galactopyranoside | 8.3 | 861 | 0.01 | pH 6.5, 80 °C | Open record: CelB (β-glycosidase from Pyrococcus furiosus), methyl beta-D-galactopyranoside |
| SsβGly (β-glycosidase from Sulfolobus solfataricus) | Sulfolobus solfataricus | 2-nitrophenyl beta-D-galactopyranoside | 1.3 × 10³ | 1.1 | 1.2 × 10³ | pH 6.5, 80 °C | Open record: SsβGly (β-glycosidase from Sulfolobus solfataricus), 2-nitrophenyl beta-D-galactopyranoside |
| SsβGly (β-glycosidase from Sulfolobus solfataricus) | Sulfolobus solfataricus | 2-nitrophenyl beta-D-glucopyranoside | — | 0.8 | — | Open record: SsβGly (β-glycosidase from Sulfolobus solfataricus), 2-nitrophenyl beta-D-glucopyranoside | |
| SsβGly (β-glycosidase from Sulfolobus solfataricus) | Sulfolobus solfataricus | beta-D-galactopyranosyl-(1->4)-D-glucose | 1.5 × 10³ | 196 | 7.7 | pH 6.5, 80 °C | Open record: SsβGly (β-glycosidase from Sulfolobus solfataricus), beta-D-galactopyranosyl-(1->4)-D-glucose |
| SsβGly (β-glycosidase from Sulfolobus solfataricus) | Sulfolobus solfataricus | methyl beta-D-galactopyranoside | 6.6 | 192 | 0.034 | pH 6.5, 80 °C | Open record: SsβGly (β-glycosidase from Sulfolobus solfataricus), methyl beta-D-galactopyranoside |