Values CatLog read from this paper
Analysis of the kinetic and redox properties of the NADH peroxidase R303M mutant: correlation with the crystal structure
PMID 10956025doi:10.1021/bi000553mOpen in Browse
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CatLog read 8 records (12 values) from this paper: NADH peroxidase from Enterococcus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 2 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| NADH peroxidasevariant R303M | Enterococcus | (4S)-[4-1H]NADH | 2.3 | — | — | pH 7, 5 °C | Open record: NADH peroxidase variant R303M, (4S)-[4-1H]NADH |
| NADH peroxidasevariant R303M | Enterococcus | (4S)-[4-2H]NADH | 1.8 | — | — | pH 7, 5 °C | Open record: NADH peroxidase variant R303M, (4S)-[4-2H]NADH |
| NADH peroxidasevariant R303M | Enterococcus | 3-acetylpyridine adenine dinucleotide, reduced | 0.1 | — | — | pH 7, 5 °C | Open record: NADH peroxidase variant R303M, 3-acetylpyridine adenine dinucleotide, reduced |
| NADH peroxidasevariant R303M | Enterococcus | hydrogen peroxide50 mM potassium phosphate; 0.1 M potassium acetate | 3 | 6.4 | 0.47 calc. | pH 7, 5 °C | Open record: NADH peroxidase variant R303M, hydrogen peroxide |
| NADH peroxidasevariant R303M | Enterococcus | hydrogen peroxide | 8.2 | 5.7 | 1.4 | pH 5.4, 25 °C | Open record: NADH peroxidase variant R303M, hydrogen peroxide |
| NADH peroxidasevariant R303M | Enterococcus | hydrogen peroxideinitial rates of AcPyADH oxidation at 66 µM AcPyADH | 0.1 | 0.1 | 1.0 calc. | pH 7, 5 °C | Open record: NADH peroxidase variant R303M, hydrogen peroxide |
| NADH peroxidasevariant R303M | Enterococcus | NADH50 mM potassium phosphate; 0.1 M potassium acetate | — | 0.001 | — | pH 7, 5 °C | Open record: NADH peroxidase variant R303M, NADH |
| NADH peroxidasevariant R303M | Enterococcus | NADHenzyme-monitored turnover in the stopped-flow: 21 µM enzyme | 2 | — | — | pH 7, 5 °C | Open record: NADH peroxidase variant R303M, NADH |