Values CatLog read from this paper
Identification of the active site acid/base catalyst in a bacterial fumarate reductase: a kinetic and crystallographic study
PMID 10978153doi:10.1021/bi000871lOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 14 records (28 values) from this paper: flavocytochrome c3 (Fcc3), soluble periplasmic fumarate reductase from Shewanella frigidimarina.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 14 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| flavocytochrome c3 (Fcc3), soluble periplasmic fumarate reductase | Shewanella frigidimarina | fumarate | 370 | 0.028 | 1.3 × 10⁴ calc. | pH 7.5, 25 °C | Open record: flavocytochrome c3 (Fcc3), soluble periplasmic fumarate reductase, fumarate |
| flavocytochrome c3 (Fcc3), soluble periplasmic fumarate reductase | Shewanella frigidimarina | fumarate | 210 | 0.007 | 3.0 × 10⁴ calc. | pH 9, 25 °C | Open record: flavocytochrome c3 (Fcc3), soluble periplasmic fumarate reductase, fumarate |
| flavocytochrome c3 (Fcc3), soluble periplasmic fumarate reductasevariant H365A | Shewanella frigidimarina | fumarate | 47 | 0.113 | 420 calc. | pH 6, 25 °C | Open record: flavocytochrome c3 (Fcc3), soluble periplasmic fumarate reductase variant H365A, fumarate |
| flavocytochrome c3 (Fcc3), soluble periplasmic fumarate reductasevariant H365A | Shewanella frigidimarina | fumarate | 51 | 0.259 | 200 calc. | pH 7.2, 25 °C | Open record: flavocytochrome c3 (Fcc3), soluble periplasmic fumarate reductase variant H365A, fumarate |
| flavocytochrome c3 (Fcc3), soluble periplasmic fumarate reductasevariant H365A | Shewanella frigidimarina | fumarate | 54 | 0.143 | 380 calc. | pH 7.5, 25 °C | Open record: flavocytochrome c3 (Fcc3), soluble periplasmic fumarate reductase variant H365A, fumarate |
| flavocytochrome c3 (Fcc3), soluble periplasmic fumarate reductasevariant H365A | Shewanella frigidimarina | fumarate | 52 | 0.224 | 230 calc. | pH 9, 25 °C | Open record: flavocytochrome c3 (Fcc3), soluble periplasmic fumarate reductase variant H365A, fumarate |
| flavocytochrome c3 (Fcc3), soluble periplasmic fumarate reductasevariant H365A/H504A | Shewanella frigidimarina | fumarate | 0.28 | 0.08 | 3.5 calc. | pH 6, 25 °C | Open record: flavocytochrome c3 (Fcc3), soluble periplasmic fumarate reductase variant H365A/H504A, fumarate |
| flavocytochrome c3 (Fcc3), soluble periplasmic fumarate reductasevariant H365A/H504A | Shewanella frigidimarina | fumarate | 0.84 | 1.1 | 0.76 calc. | pH 7.2, 25 °C | Open record: flavocytochrome c3 (Fcc3), soluble periplasmic fumarate reductase variant H365A/H504A, fumarate |
| flavocytochrome c3 (Fcc3), soluble periplasmic fumarate reductasevariant H365A/H504A | Shewanella frigidimarina | fumarate | 0.95 | 1.8 | 0.53 calc. | pH 7.5, 25 °C | Open record: flavocytochrome c3 (Fcc3), soluble periplasmic fumarate reductase variant H365A/H504A, fumarate |
| flavocytochrome c3 (Fcc3), soluble periplasmic fumarate reductasevariant H365A/H504A | Shewanella frigidimarina | fumarate | 0.95 | 1.3 | 0.73 calc. | pH 9, 25 °C | Open record: flavocytochrome c3 (Fcc3), soluble periplasmic fumarate reductase variant H365A/H504A, fumarate |
| flavocytochrome c3 (Fcc3), soluble periplasmic fumarate reductasevariant H504A | Shewanella frigidimarina | fumarate | 26 | 0.038 | 680 calc. | pH 6, 25 °C | Open record: flavocytochrome c3 (Fcc3), soluble periplasmic fumarate reductase variant H504A, fumarate |
| flavocytochrome c3 (Fcc3), soluble periplasmic fumarate reductasevariant H504A | Shewanella frigidimarina | fumarate | 65 | 0.256 | 250 calc. | pH 7.2, 25 °C | Open record: flavocytochrome c3 (Fcc3), soluble periplasmic fumarate reductase variant H504A, fumarate |
| flavocytochrome c3 (Fcc3), soluble periplasmic fumarate reductasevariant H504A | Shewanella frigidimarina | fumarate | 68 | 0.2 | 340 calc. | pH 7.5, 25 °C | Open record: flavocytochrome c3 (Fcc3), soluble periplasmic fumarate reductase variant H504A, fumarate |
| flavocytochrome c3 (Fcc3), soluble periplasmic fumarate reductasevariant H504A | Shewanella frigidimarina | fumarate | 76 | 0.635 | 120 calc. | pH 9, 25 °C | Open record: flavocytochrome c3 (Fcc3), soluble periplasmic fumarate reductase variant H504A, fumarate |