Values CatLog read from this paper
Conversion of methylamine dehydrogenase to a long-chain amine dehydrogenase by mutagenesis of a single residue
PMID 10985763doi:10.1021/bi001568nOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 22 records (44 values) from this paper: methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent) from Paracoccus denitrificans.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 22 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent) | Paracoccus denitrificans | 1,10-diaminodecane | 21 | 1.2 | 18 calc. | pH 7.5, 30 °C | Open record: methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent), 1,10-diaminodecane |
| methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent) | Paracoccus denitrificans | 1,5-diaminopentane | 22 | 0.82 | 27 calc. | pH 7.5, 30 °C | Open record: methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent), 1,5-diaminopentane |
| methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent) | Paracoccus denitrificans | 1,6-diaminohexane | 17 | 0.72 | 24 calc. | pH 7.5, 30 °C | Open record: methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent), 1,6-diaminohexane |
| methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent) | Paracoccus denitrificans | 1,7-diaminoheptane | 27 | 0.38 | 71 calc. | pH 7.5, 30 °C | Open record: methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent), 1,7-diaminoheptane |
| methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent) | Paracoccus denitrificans | 1,8-diaminooctane | 11 | 0.34 | 32 calc. | pH 7.5, 30 °C | Open record: methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent), 1,8-diaminooctane |
| methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent) | Paracoccus denitrificans | 1,9-diaminononane | 17 | 0.83 | 20 calc. | pH 7.5, 30 °C | Open record: methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent), 1,9-diaminononane |
| methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent) | Paracoccus denitrificans | 1-aminopentane | 17 | 2.5 | 6.8 calc. | pH 7.5, 30 °C | Open record: methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent), 1-aminopentane |
| methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent) | Paracoccus denitrificans | butylamine | 22 | 0.87 | 25 calc. | pH 7.5, 30 °C | Open record: methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent), butylamine |
| methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent) | Paracoccus denitrificans | ethylamine | 24 | 0.019 | 1.3 × 10³ calc. | pH 7.5, 30 °C | Open record: methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent), ethylamine |
| methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent) | Paracoccus denitrificans | methylamine | 30 | 0.009 | 3.3 × 10³ calc. | pH 7.5, 30 °C | Open record: methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent), methylamine |
| methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent) | Paracoccus denitrificans | propylamine | 27 | 0.036 | 750 calc. | pH 7.5, 30 °C | Open record: methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent), propylamine |
| methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent)variant αF55A | Paracoccus denitrificans | 1,10-diaminodecane | 41 | 0.011 | 3.7 × 10³ calc. | pH 7.5, 30 °C | Open record: methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent) variant αF55A, 1,10-diaminodecane |
| methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent)variant αF55A | Paracoccus denitrificans | 1,5-diaminopentane | 59 | 0.059 | 1.0 × 10³ calc. | pH 7.5, 30 °C | Open record: methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent) variant αF55A, 1,5-diaminopentane |
| methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent)variant αF55A | Paracoccus denitrificans | 1,6-diaminohexane | 43 | 0.021 | 2.0 × 10³ calc. | pH 7.5, 30 °C | Open record: methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent) variant αF55A, 1,6-diaminohexane |
| methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent)variant αF55A | Paracoccus denitrificans | 1,7-diaminoheptane | 32 | 0.007 | 4.6 × 10³ calc. | pH 7.5, 30 °C | Open record: methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent) variant αF55A, 1,7-diaminoheptane |
| methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent)variant αF55A | Paracoccus denitrificans | 1,8-diaminooctane | 38 | 0.009 | 4.2 × 10³ calc. | pH 7.5, 30 °C | Open record: methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent) variant αF55A, 1,8-diaminooctane |
| methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent)variant αF55A | Paracoccus denitrificans | 1,9-diaminononane | 39 | 0.009 | 4.3 × 10³ calc. | pH 7.5, 30 °C | Open record: methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent) variant αF55A, 1,9-diaminononane |
| methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent)variant αF55A | Paracoccus denitrificans | 1-aminopentane | 20 | 0.047 | 430 calc. | pH 7.5, 30 °C | Open record: methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent) variant αF55A, 1-aminopentane |
| methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent)variant αF55A | Paracoccus denitrificans | butylamine | 34 | 0.24 | 140 calc. | pH 7.5, 30 °C | Open record: methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent) variant αF55A, butylamine |
| methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent)variant αF55A | Paracoccus denitrificans | ethylamine | 23 | 9.2 | 2.5 calc. | pH 7.5, 30 °C | Open record: methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent) variant αF55A, ethylamine |
| methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent)variant αF55A | Paracoccus denitrificans | methylamine | 77 | 14.9 | 5.2 calc. | pH 7.5, 30 °C | Open record: methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent) variant αF55A, methylamine |
| methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent)variant αF55A | Paracoccus denitrificans | propylamine | 24 | 1.3 | 18 calc. | pH 7.5, 30 °C | Open record: methylamine dehydrogenase (MADH, α2β2 heterotetramer, TTQ-dependent) variant αF55A, propylamine |