Values CatLog read from this paper

The tRNA-dependent activation of arginine by arginyl-tRNA synthetase requires inter-domain communication

PMID 10993737doi:10.1006/jmbi.2000.4102Open in Browse

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CatLog read 64 records (160 values) from this paper: arginyl-tRNA synthetase (ArgRS) from Cricetulus longicaudatus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 6.1.1.19

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
arginyl-tRNA synthetase (ArgRS)	Cricetulus longicaudatus	adenosine 5'-triphosphate	1.4	0.194	7.2	pH 7.5, 25 °C	Open record: arginyl-tRNA synthetase (ArgRS), adenosine 5'-triphosphate
arginyl-tRNA synthetase (ArgRS)	Cricetulus longicaudatus	adenosine 5'-triphosphate	2.5	0.24	10	pH 7.5, 37 °C	Open record: arginyl-tRNA synthetase (ArgRS), adenosine 5'-triphosphate
arginyl-tRNA synthetase (ArgRS)	Cricetulus longicaudatus	adenosine 5'-triphosphate	2.1	0.152	14	pH 7.5, 25 °C	Open record: arginyl-tRNA synthetase (ArgRS), adenosine 5'-triphosphate
arginyl-tRNA synthetase (ArgRS)	Cricetulus longicaudatus	adenosine 5'-triphosphate	3.8	0.161	24	pH 7.5, 37 °C	Open record: arginyl-tRNA synthetase (ArgRS), adenosine 5'-triphosphate
arginyl-tRNA synthetase (ArgRS)	Cricetulus longicaudatus	L-arginineATP-PPi exchange assay ([32P]PPi-ATP exchange, 10 min)	10.2	7 × 10⁻⁴	1.45 × 10⁴	pH 7.5, 25 °C	Open record: arginyl-tRNA synthetase (ArgRS), L-arginine
arginyl-tRNA synthetase (ArgRS)	Cricetulus longicaudatus	L-arginineATP-PPi exchange assay ([32P]PPi-ATP exchange, 10 min)	22	0.0023	9.6 × 10³	pH 7.5, 37 °C	Open record: arginyl-tRNA synthetase (ArgRS), L-arginine
arginyl-tRNA synthetase (ArgRS)	Cricetulus longicaudatus	L-arginineATP-PPi exchange assay ([32P]PPi-ATP exchange, 10 min)	11.5	0.0011	1.05 × 10⁴	pH 7.5, 25 °C	Open record: arginyl-tRNA synthetase (ArgRS), L-arginine
arginyl-tRNA synthetase (ArgRS)	Cricetulus longicaudatus	L-arginineATP-PPi exchange assay ([32P]PPi-ATP exchange, 10 min)	28	0.0028	1 × 10⁴	pH 7.5, 37 °C	Open record: arginyl-tRNA synthetase (ArgRS), L-arginine
arginyl-tRNA synthetase (ArgRS)	Cricetulus longicaudatus	L-argininetRNA aminoacylation assay; 150 mM KCl; 5 mM MgCl2; 3 mM ATP	1.4	0.0013	1.1 × 10³	pH 7.5, 25 °C	Open record: arginyl-tRNA synthetase (ArgRS), L-arginine
arginyl-tRNA synthetase (ArgRS)	Cricetulus longicaudatus	L-argininetRNA aminoacylation assay; 150 mM KCl; 5 mM MgCl2; 3 mM ATP	2.5	0.0024	1 × 10³	pH 7.5, 37 °C	Open record: arginyl-tRNA synthetase (ArgRS), L-arginine
arginyl-tRNA synthetase (ArgRS)	Cricetulus longicaudatus	L-argininetRNA aminoacylation assay; 150 mM KCl; 5 mM MgCl2; 3 mM ATP	1.9	0.0015	1.3 × 10³	pH 7.5, 25 °C	Open record: arginyl-tRNA synthetase (ArgRS), L-arginine
arginyl-tRNA synthetase (ArgRS)	Cricetulus longicaudatus	L-argininetRNA aminoacylation assay; 150 mM KCl; 5 mM MgCl2; 3 mM ATP	2.3	0.0025	900	pH 7.5, 37 °C	Open record: arginyl-tRNA synthetase (ArgRS), L-arginine
arginyl-tRNA synthetase (ArgRS)	Cricetulus longicaudatus	tRNA(Arg) (arginine-specific tRNA from beef liver)	1.8	3.2 × 10⁻⁴	5.6 × 10³	pH 7.5, 25 °C	Open record: arginyl-tRNA synthetase (ArgRS), tRNA(Arg) (arginine-specific tRNA from beef liver)
arginyl-tRNA synthetase (ArgRS)	Cricetulus longicaudatus	tRNA(Arg) (arginine-specific tRNA from beef liver)	3	4.4 × 10⁻⁴	6.8 × 10³	pH 7.5, 37 °C	Open record: arginyl-tRNA synthetase (ArgRS), tRNA(Arg) (arginine-specific tRNA from beef liver)
arginyl-tRNA synthetase (ArgRS)	Cricetulus longicaudatus	tRNA(Arg) (arginine-specific tRNA from beef liver)	2.6	3.9 × 10⁻⁴	6.6 × 10³	pH 7.5, 25 °C	Open record: arginyl-tRNA synthetase (ArgRS), tRNA(Arg) (arginine-specific tRNA from beef liver)
arginyl-tRNA synthetase (ArgRS)	Cricetulus longicaudatus	tRNA(Arg) (arginine-specific tRNA from beef liver)	4.1	4.1 × 10⁻⁴	1 × 10⁴	pH 7.5, 37 °C	Open record: arginyl-tRNA synthetase (ArgRS), tRNA(Arg) (arginine-specific tRNA from beef liver)
arginyl-tRNA synthetase (ArgRS)variant C599Y	Cricetulus longicaudatus	adenosine 5'-triphosphate	0.15	0.102	1.5	pH 7.5, 25 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant C599Y, adenosine 5'-triphosphate
arginyl-tRNA synthetase (ArgRS)variant C599Y	Cricetulus longicaudatus	adenosine 5'-triphosphate	0.07	0.143	0.5	pH 7.5, 37 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant C599Y, adenosine 5'-triphosphate
arginyl-tRNA synthetase (ArgRS)variant C599Y	Cricetulus longicaudatus	L-arginineATP-PPi exchange assay ([32P]PPi-ATP exchange, 10 min)	2.6	0.0017	1.5 × 10³	pH 7.5, 25 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant C599Y, L-arginine
arginyl-tRNA synthetase (ArgRS)variant C599Y	Cricetulus longicaudatus	L-arginineATP-PPi exchange assay ([32P]PPi-ATP exchange, 10 min)	3.8	0.0024	1.6 × 10³	pH 7.5, 37 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant C599Y, L-arginine
arginyl-tRNA synthetase (ArgRS)variant C599Y	Cricetulus longicaudatus	L-argininetRNA aminoacylation assay; 150 mM KCl; 5 mM MgCl2; 3 mM ATP	0.06	—	—	pH 7.5, 25 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant C599Y, L-arginine
arginyl-tRNA synthetase (ArgRS)variant C599Y	Cricetulus longicaudatus	L-argininetRNA aminoacylation assay; 150 mM KCl; 5 mM MgCl2; 3 mM ATP	0.25	—	—	pH 7.5, 25 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant C599Y, L-arginine
arginyl-tRNA synthetase (ArgRS)variant C599Y	Cricetulus longicaudatus	L-argininetRNA aminoacylation assay; 150 mM KCl; 5 mM MgCl2; 3 mM ATP	0.04	—	—	pH 7.5, 37 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant C599Y, L-arginine
arginyl-tRNA synthetase (ArgRS)variant C599Y	Cricetulus longicaudatus	L-argininetRNA aminoacylation assay; 150 mM KCl; 5 mM MgCl2; 3 mM ATP	0.23	—	—	pH 7.5, 37 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant C599Y, L-arginine
arginyl-tRNA synthetase (ArgRS)variant C599Y	Cricetulus longicaudatus	tRNA(Arg) (arginine-specific tRNA from beef liver)	0.21	3.7 × 10⁻⁴	570	pH 7.5, 25 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant C599Y, tRNA(Arg) (arginine-specific tRNA from beef liver)
arginyl-tRNA synthetase (ArgRS)variant C599Y	Cricetulus longicaudatus	tRNA(Arg) (arginine-specific tRNA from beef liver)	0.24	0.00125	190	pH 7.5, 37 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant C599Y, tRNA(Arg) (arginine-specific tRNA from beef liver)
arginyl-tRNA synthetase (ArgRS)variant C599Y/M222I	Cricetulus longicaudatus	adenosine 5'-triphosphate	0.11	0.053	2.1	pH 7.5, 25 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant C599Y/M222I, adenosine 5'-triphosphate
arginyl-tRNA synthetase (ArgRS)variant C599Y/M222I	Cricetulus longicaudatus	adenosine 5'-triphosphate	0.1	0.079	1.3	pH 7.5, 37 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant C599Y/M222I, adenosine 5'-triphosphate
arginyl-tRNA synthetase (ArgRS)variant C599Y/M222I	Cricetulus longicaudatus	L-arginineATP-PPi exchange assay ([32P]PPi-ATP exchange, 10 min)	2.9	0.0015	1.9 × 10³	pH 7.5, 25 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant C599Y/M222I, L-arginine
arginyl-tRNA synthetase (ArgRS)variant C599Y/M222I	Cricetulus longicaudatus	L-arginineATP-PPi exchange assay ([32P]PPi-ATP exchange, 10 min)	5.6	0.0028	2 × 10³	pH 7.5, 37 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant C599Y/M222I, L-arginine
arginyl-tRNA synthetase (ArgRS)variant C599Y/M222I	Cricetulus longicaudatus	L-argininetRNA aminoacylation assay; 150 mM KCl; 5 mM MgCl2; 3 mM ATP	0.05	—	—	pH 7.5, 25 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant C599Y/M222I, L-arginine
arginyl-tRNA synthetase (ArgRS)variant C599Y/M222I	Cricetulus longicaudatus	L-argininetRNA aminoacylation assay; 150 mM KCl; 5 mM MgCl2; 3 mM ATP	0.25	—	—	pH 7.5, 25 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant C599Y/M222I, L-arginine
arginyl-tRNA synthetase (ArgRS)variant C599Y/M222I	Cricetulus longicaudatus	L-argininetRNA aminoacylation assay; 150 mM KCl; 5 mM MgCl2; 3 mM ATP	0.04	—	—	pH 7.5, 37 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant C599Y/M222I, L-arginine
arginyl-tRNA synthetase (ArgRS)variant C599Y/M222I	Cricetulus longicaudatus	L-argininetRNA aminoacylation assay; 150 mM KCl; 5 mM MgCl2; 3 mM ATP	0.14	—	—	pH 7.5, 37 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant C599Y/M222I, L-arginine
arginyl-tRNA synthetase (ArgRS)variant C599Y/M222I	Cricetulus longicaudatus	tRNA(Arg) (arginine-specific tRNA from beef liver)	0.14	2.9 × 10⁻⁴	480	pH 7.5, 25 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant C599Y/M222I, tRNA(Arg) (arginine-specific tRNA from beef liver)
arginyl-tRNA synthetase (ArgRS)variant C599Y/M222I	Cricetulus longicaudatus	tRNA(Arg) (arginine-specific tRNA from beef liver)	0.16	0.00132	120	pH 7.5, 37 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant C599Y/M222I, tRNA(Arg) (arginine-specific tRNA from beef liver)
arginyl-tRNA synthetase (ArgRS)variant C599Y/Y506S	Cricetulus longicaudatus	adenosine 5'-triphosphate	0.08	0.0072	11	pH 7.5, 25 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant C599Y/Y506S, adenosine 5'-triphosphate
arginyl-tRNA synthetase (ArgRS)variant C599Y/Y506S	Cricetulus longicaudatus	adenosine 5'-triphosphate	0.11	0.009	12	pH 7.5, 37 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant C599Y/Y506S, adenosine 5'-triphosphate
arginyl-tRNA synthetase (ArgRS)variant C599Y/Y506S	Cricetulus longicaudatus	L-arginineATP-PPi exchange assay ([32P]PPi-ATP exchange, 10 min)	8.6	4 × 10⁻⁴	2.15 × 10⁴	pH 7.5, 25 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant C599Y/Y506S, L-arginine
arginyl-tRNA synthetase (ArgRS)variant C599Y/Y506S	Cricetulus longicaudatus	L-arginineATP-PPi exchange assay ([32P]PPi-ATP exchange, 10 min)	12.4	6 × 10⁻⁴	2.07 × 10⁴	pH 7.5, 37 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant C599Y/Y506S, L-arginine
arginyl-tRNA synthetase (ArgRS)variant C599Y/Y506S	Cricetulus longicaudatus	L-argininetRNA aminoacylation assay; 150 mM KCl; 5 mM MgCl2; 3 mM ATP	0.08	—	—	pH 7.5, 25 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant C599Y/Y506S, L-arginine
arginyl-tRNA synthetase (ArgRS)variant C599Y/Y506S	Cricetulus longicaudatus	L-argininetRNA aminoacylation assay; 150 mM KCl; 5 mM MgCl2; 3 mM ATP	0.37	—	—	pH 7.5, 25 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant C599Y/Y506S, L-arginine
arginyl-tRNA synthetase (ArgRS)variant C599Y/Y506S	Cricetulus longicaudatus	L-argininetRNA aminoacylation assay; 150 mM KCl; 5 mM MgCl2; 3 mM ATP	0.13	—	—	pH 7.5, 37 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant C599Y/Y506S, L-arginine
arginyl-tRNA synthetase (ArgRS)variant C599Y/Y506S	Cricetulus longicaudatus	L-argininetRNA aminoacylation assay; 150 mM KCl; 5 mM MgCl2; 3 mM ATP	0.54	—	—	pH 7.5, 37 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant C599Y/Y506S, L-arginine
arginyl-tRNA synthetase (ArgRS)variant C599Y/Y506S	Cricetulus longicaudatus	tRNA(Arg) (arginine-specific tRNA from beef liver)	0.29	2.6 × 10⁻⁴	1.2 × 10³	pH 7.5, 25 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant C599Y/Y506S, tRNA(Arg) (arginine-specific tRNA from beef liver)
arginyl-tRNA synthetase (ArgRS)variant C599Y/Y506S	Cricetulus longicaudatus	tRNA(Arg) (arginine-specific tRNA from beef liver)	0.5	3.5 × 10⁻⁴	1.4 × 10³	pH 7.5, 37 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant C599Y/Y506S, tRNA(Arg) (arginine-specific tRNA from beef liver)
arginyl-tRNA synthetase (ArgRS)variant M222I	Cricetulus longicaudatus	adenosine 5'-triphosphate	2.2	0.048	46	pH 7.5, 25 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant M222I, adenosine 5'-triphosphate
arginyl-tRNA synthetase (ArgRS)variant M222I	Cricetulus longicaudatus	adenosine 5'-triphosphate	2.9	0.054	54	pH 7.5, 37 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant M222I, adenosine 5'-triphosphate
arginyl-tRNA synthetase (ArgRS)variant M222I	Cricetulus longicaudatus	L-arginineATP-PPi exchange assay ([32P]PPi-ATP exchange, 10 min)	2.4	0.001	2.4 × 10³	pH 7.5, 25 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant M222I, L-arginine
arginyl-tRNA synthetase (ArgRS)variant M222I	Cricetulus longicaudatus	L-arginineATP-PPi exchange assay ([32P]PPi-ATP exchange, 10 min)	6	0.0028	2.1 × 10³	pH 7.5, 37 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant M222I, L-arginine
arginyl-tRNA synthetase (ArgRS)variant M222I	Cricetulus longicaudatus	L-argininetRNA aminoacylation assay; 150 mM KCl; 5 mM MgCl2; 3 mM ATP	1.9	0.0014	1.36 × 10³	pH 7.5, 25 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant M222I, L-arginine
arginyl-tRNA synthetase (ArgRS)variant M222I	Cricetulus longicaudatus	L-argininetRNA aminoacylation assay; 150 mM KCl; 5 mM MgCl2; 3 mM ATP	2.7	0.0018	1.5 × 10³	pH 7.5, 37 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant M222I, L-arginine
arginyl-tRNA synthetase (ArgRS)variant M222I	Cricetulus longicaudatus	tRNA(Arg) (arginine-specific tRNA from beef liver)	1.9	2.4 × 10⁻⁴	7.9 × 10³	pH 7.5, 25 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant M222I, tRNA(Arg) (arginine-specific tRNA from beef liver)
arginyl-tRNA synthetase (ArgRS)variant M222I	Cricetulus longicaudatus	tRNA(Arg) (arginine-specific tRNA from beef liver)	3.2	2.8 × 10⁻⁴	1.14 × 10⁴	pH 7.5, 37 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant M222I, tRNA(Arg) (arginine-specific tRNA from beef liver)
arginyl-tRNA synthetase (ArgRS)variant Y506S	Cricetulus longicaudatus	adenosine 5'-triphosphate	0.51	0.046	11.1	pH 7.5, 25 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant Y506S, adenosine 5'-triphosphate
arginyl-tRNA synthetase (ArgRS)variant Y506S	Cricetulus longicaudatus	adenosine 5'-triphosphate	0.37	0.054	6.9	pH 7.5, 37 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant Y506S, adenosine 5'-triphosphate
arginyl-tRNA synthetase (ArgRS)variant Y506S	Cricetulus longicaudatus	L-arginineATP-PPi exchange assay ([32P]PPi-ATP exchange, 10 min)	9.7	7 × 10⁻⁴	1.39 × 10⁴	pH 7.5, 25 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant Y506S, L-arginine
arginyl-tRNA synthetase (ArgRS)variant Y506S	Cricetulus longicaudatus	L-arginineATP-PPi exchange assay ([32P]PPi-ATP exchange, 10 min)	14.3	0.0014	1.02 × 10⁴	pH 7.5, 37 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant Y506S, L-arginine
arginyl-tRNA synthetase (ArgRS)variant Y506S	Cricetulus longicaudatus	L-argininetRNA aminoacylation assay; 150 mM KCl; 5 mM MgCl2; 3 mM ATP	0.2	—	—	pH 7.5, 25 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant Y506S, L-arginine
arginyl-tRNA synthetase (ArgRS)variant Y506S	Cricetulus longicaudatus	L-argininetRNA aminoacylation assay; 150 mM KCl; 5 mM MgCl2; 3 mM ATP	0.75	—	—	pH 7.5, 25 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant Y506S, L-arginine
arginyl-tRNA synthetase (ArgRS)variant Y506S	Cricetulus longicaudatus	L-argininetRNA aminoacylation assay; 150 mM KCl; 5 mM MgCl2; 3 mM ATP	0.19	—	—	pH 7.5, 37 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant Y506S, L-arginine
arginyl-tRNA synthetase (ArgRS)variant Y506S	Cricetulus longicaudatus	L-argininetRNA aminoacylation assay; 150 mM KCl; 5 mM MgCl2; 3 mM ATP	0.77	—	—	pH 7.5, 37 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant Y506S, L-arginine
arginyl-tRNA synthetase (ArgRS)variant Y506S	Cricetulus longicaudatus	tRNA(Arg) (arginine-specific tRNA from beef liver)	0.71	3.3 × 10⁻⁴	2.15 × 10³	pH 7.5, 25 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant Y506S, tRNA(Arg) (arginine-specific tRNA from beef liver)
arginyl-tRNA synthetase (ArgRS)variant Y506S	Cricetulus longicaudatus	tRNA(Arg) (arginine-specific tRNA from beef liver)	0.74	9.5 × 10⁻⁴	780	pH 7.5, 37 °C	Open record: arginyl-tRNA synthetase (ArgRS) variant Y506S, tRNA(Arg) (arginine-specific tRNA from beef liver)