CatLogenzyme kinetics catalog

Values CatLog read from this paper

Probing the NADPH-binding site of Escherichia coli flavodoxin oxidoreductase

PMID 11085917doi:10.1042/bj3520257Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 20 records (29 values) from this paper: flavodoxin NADP+ oxidoreductase (FLDR) from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.

EC 1.18.1.2

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
flavodoxin NADP+ oxidoreductase (FLDR)Escherichia colicytochrome cNADPH 200 µM (saturating); cosubstrate NADPH5.6480.0176321 calc.pH 7.5, 30 °COpen
flavodoxin NADP+ oxidoreductase (FLDR)Escherichia colicytochrome cNADH 10 mM (near-saturating); cosubstrate NADH0.55——pH 7.5, 30 °COpen
flavodoxin NADP+ oxidoreductase (FLDR)Escherichia colicytochrome cNADH 10 mM (near-saturating); cosubstrate NADH; apparent Km—0.0032—pH 7.5, 30 °COpen
flavodoxin NADP+ oxidoreductase (FLDR)Escherichia coliNADH—2.040.27pH 7.5, 30 °COpen
flavodoxin NADP+ oxidoreductase (FLDR)Escherichia coliNADPH—0.00391448pH 7.5, 30 °COpen
flavodoxin NADP+ oxidoreductase (FLDR)variant R144AEscherichia colicytochrome cNADPH 200 µM (saturating); cosubstrate NADPH4.027——pH 7.5, 30 °COpen
flavodoxin NADP+ oxidoreductase (FLDR)variant R144AEscherichia colicytochrome cNADH 10 mM (near-saturating); cosubstrate NADH0.228——pH 7.5, 30 °COpen
flavodoxin NADP+ oxidoreductase (FLDR)variant R144AEscherichia colicytochrome cNADH 10 mM (near-saturating); cosubstrate NADH; apparent Km—8.2 × 10⁻⁴—pH 7.5, 30 °COpen
flavodoxin NADP+ oxidoreductase (FLDR)variant R144AEscherichia coliNADH—5.140.0445pH 7.5, 30 °COpen
flavodoxin NADP+ oxidoreductase (FLDR)variant R144AEscherichia coliNADPH—0.0053762pH 7.5, 30 °COpen
flavodoxin NADP+ oxidoreductase (FLDR)variant R174AEscherichia colicytochrome cNADPH 200 µM (saturating); cosubstrate NADPH2.202——pH 7.5, 30 °COpen
flavodoxin NADP+ oxidoreductase (FLDR)variant R174AEscherichia colicytochrome cNADH 10 mM (near-saturating); cosubstrate NADH0.705——pH 7.5, 30 °COpen
flavodoxin NADP+ oxidoreductase (FLDR)variant R174AEscherichia colicytochrome cNADH 10 mM (near-saturating); cosubstrate NADH; apparent Km—0.0059—pH 7.5, 30 °COpen
flavodoxin NADP+ oxidoreductase (FLDR)variant R174AEscherichia coliNADH—9.860.0715pH 7.5, 30 °COpen
flavodoxin NADP+ oxidoreductase (FLDR)variant R174AEscherichia coliNADPH—0.0202108pH 7.5, 30 °COpen
flavodoxin NADP+ oxidoreductase (FLDR)variant R184AEscherichia colicytochrome cNADPH 200 µM (saturating); cosubstrate NADPH5.092——pH 7.5, 30 °COpen
flavodoxin NADP+ oxidoreductase (FLDR)variant R184AEscherichia colicytochrome cNADH 10 mM (near-saturating); cosubstrate NADH0.84——pH 7.5, 30 °COpen
flavodoxin NADP+ oxidoreductase (FLDR)variant R184AEscherichia colicytochrome cNADH 10 mM (near-saturating); cosubstrate NADH; apparent Km—0.0044—pH 7.5, 30 °COpen
flavodoxin NADP+ oxidoreductase (FLDR)variant R184AEscherichia coliNADH—1.660.507pH 7.5, 30 °COpen
flavodoxin NADP+ oxidoreductase (FLDR)variant R184AEscherichia coliNADPH—0.054493pH 7.5, 30 °COpen