Values CatLog read from this paper
Modification of the substrate specificity of porcine pepsin for the enzymatic production of bovine hide gelatin
PMID 11106168doi:10.1110/ps.9.10.1947Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 45 records (135 values) from this paper: pepsin from Sus scrofa.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| pepsin | Sus scrofa | Lys-Pro-Ile-Gln-Phe-(p-nitrophenylalanine)-Arg-Leu | 47.9 | 0.046 | 1.04 × 10³ | pH 3.5, 25 °C | Open record: pepsin, Lys-Pro-Ile-Gln-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsin | Sus scrofa | Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | 68.9 | 0.035 | 1.97 × 10³ | pH 3.5, 25 °C | Open record: pepsin, Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsin | Sus scrofa | Lys-Pro-Ile-Leu-Phe-(p-nitrophenylalanine)-Arg-Leu | 14 | 0.0081 | 1.75 × 10³ | pH 3.5, 25 °C | Open record: pepsin, Lys-Pro-Ile-Leu-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsin | Sus scrofa | Lys-Pro-Ile-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu | 38.9 | 0.079 | 490 | pH 3.5, 25 °C | Open record: pepsin, Lys-Pro-Ile-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsin | Sus scrofa | Lys-Pro-Pro-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | 47.3 | 0.053 | 890 | pH 3.5, 25 °C | Open record: pepsin, Lys-Pro-Pro-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsinS-M loop mutant (pepsin loop S238-M245 replaced by the corresponding human cathepsin D loop V254-Y261) | Sus scrofa | Lys-Pro-Ile-Gln-Phe-(p-nitrophenylalanine)-Arg-Leu | 44.5 | 0.05 | 890 | pH 3.5, 25 °C | Open record: pepsin S-M loop mutant (pepsin loop S238-M245 replaced by the corresponding human cathepsin D loop V254-Y261), Lys-Pro-Ile-Gln-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsinS-M loop mutant (pepsin loop S238-M245 replaced by the corresponding human cathepsin D loop V254-Y261) | Sus scrofa | Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | 46 | 0.032 | 1.44 × 10³ | pH 3.5, 25 °C | Open record: pepsin S-M loop mutant (pepsin loop S238-M245 replaced by the corresponding human cathepsin D loop V254-Y261), Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsinS-M loop mutant (pepsin loop S238-M245 replaced by the corresponding human cathepsin D loop V254-Y261) | Sus scrofa | Lys-Pro-Ile-Leu-Phe-(p-nitrophenylalanine)-Arg-Leu | 21.4 | 0.0091 | 2.38 × 10³ | pH 3.5, 25 °C | Open record: pepsin S-M loop mutant (pepsin loop S238-M245 replaced by the corresponding human cathepsin D loop V254-Y261), Lys-Pro-Ile-Leu-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsinS-M loop mutant (pepsin loop S238-M245 replaced by the corresponding human cathepsin D loop V254-Y261) | Sus scrofa | Lys-Pro-Ile-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu | 27.6 | 0.08 | 350 | pH 3.5, 25 °C | Open record: pepsin S-M loop mutant (pepsin loop S238-M245 replaced by the corresponding human cathepsin D loop V254-Y261), Lys-Pro-Ile-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsinS-M loop mutant (pepsin loop S238-M245 replaced by the corresponding human cathepsin D loop V254-Y261) | Sus scrofa | Lys-Pro-Pro-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | 37.4 | 0.04 | 940 | pH 3.5, 25 °C | Open record: pepsin S-M loop mutant (pepsin loop S238-M245 replaced by the corresponding human cathepsin D loop V254-Y261), Lys-Pro-Pro-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsinY-T loop mutant (pepsin loop Y274-T283 replaced by the corresponding human cathepsin D loop Y290-L303) | Sus scrofa | Lys-Pro-Ile-Gln-Phe-(p-nitrophenylalanine)-Arg-Leu | 43.1 | 0.06 | 720 | pH 3.5, 25 °C | Open record: pepsin Y-T loop mutant (pepsin loop Y274-T283 replaced by the corresponding human cathepsin D loop Y290-L303), Lys-Pro-Ile-Gln-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsinY-T loop mutant (pepsin loop Y274-T283 replaced by the corresponding human cathepsin D loop Y290-L303) | Sus scrofa | Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | 45.5 | 0.05 | 910 | pH 3.5, 25 °C | Open record: pepsin Y-T loop mutant (pepsin loop Y274-T283 replaced by the corresponding human cathepsin D loop Y290-L303), Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsinY-T loop mutant (pepsin loop Y274-T283 replaced by the corresponding human cathepsin D loop Y290-L303) | Sus scrofa | Lys-Pro-Ile-Leu-Phe-(p-nitrophenylalanine)-Arg-Leu | 24.4 | 0.0188 | 1.28 × 10³ | pH 3.5, 25 °C | Open record: pepsin Y-T loop mutant (pepsin loop Y274-T283 replaced by the corresponding human cathepsin D loop Y290-L303), Lys-Pro-Ile-Leu-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsinY-T loop mutant (pepsin loop Y274-T283 replaced by the corresponding human cathepsin D loop Y290-L303) | Sus scrofa | Lys-Pro-Ile-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu | 28.7 | 0.142 | 200 | pH 3.5, 25 °C | Open record: pepsin Y-T loop mutant (pepsin loop Y274-T283 replaced by the corresponding human cathepsin D loop Y290-L303), Lys-Pro-Ile-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsinY-T loop mutant (pepsin loop Y274-T283 replaced by the corresponding human cathepsin D loop Y290-L303) | Sus scrofa | Lys-Pro-Pro-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | 38.5 | 0.055 | 700 | pH 3.5, 25 °C | Open record: pepsin Y-T loop mutant (pepsin loop Y274-T283 replaced by the corresponding human cathepsin D loop Y290-L303), Lys-Pro-Pro-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsinvariant E287M | Sus scrofa | Lys-Pro-Ile-Gln-Phe-(p-nitrophenylalanine)-Arg-Leu | 85.2 | 0.05 | 1.7 × 10³ | pH 3.5, 25 °C | Open record: pepsin variant E287M, Lys-Pro-Ile-Gln-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsinvariant E287M | Sus scrofa | Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | 60.7 | 0.024 | 2.53 × 10³ | pH 3.5, 25 °C | Open record: pepsin variant E287M, Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsinvariant E287M | Sus scrofa | Lys-Pro-Ile-Leu-Phe-(p-nitrophenylalanine)-Arg-Leu | 34.4 | 0.0093 | 3.82 × 10³ | pH 3.5, 25 °C | Open record: pepsin variant E287M, Lys-Pro-Ile-Leu-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsinvariant E287M | Sus scrofa | Lys-Pro-Ile-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu | 44.2 | 0.16 | 280 | pH 3.5, 25 °C | Open record: pepsin variant E287M, Lys-Pro-Ile-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsinvariant E287M | Sus scrofa | Lys-Pro-Pro-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | 62.3 | 0.074 | 840 | pH 3.5, 25 °C | Open record: pepsin variant E287M, Lys-Pro-Pro-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsinvariant F111T/L112F | Sus scrofa | Lys-Pro-Ile-Gln-Phe-(p-nitrophenylalanine)-Arg-Leu | 82.2 | 0.034 | 2.42 × 10³ | pH 3.5, 25 °C | Open record: pepsin variant F111T/L112F, Lys-Pro-Ile-Gln-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsinvariant F111T/L112F | Sus scrofa | Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | 72.5 | 0.019 | 3.82 × 10³ | pH 3.5, 25 °C | Open record: pepsin variant F111T/L112F, Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsinvariant F111T/L112F | Sus scrofa | Lys-Pro-Ile-Leu-Phe-(p-nitrophenylalanine)-Arg-Leu | 53.1 | 0.0082 | 6.64 × 10³ | pH 3.5, 25 °C | Open record: pepsin variant F111T/L112F, Lys-Pro-Ile-Leu-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsinvariant F111T/L112F | Sus scrofa | Lys-Pro-Ile-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu | 38.8 | 0.046 | 840 | pH 3.5, 25 °C | Open record: pepsin variant F111T/L112F, Lys-Pro-Ile-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsinvariant F111T/L112F | Sus scrofa | Lys-Pro-Pro-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | 62.2 | 0.024 | 2.59 × 10³ | pH 3.5, 25 °C | Open record: pepsin variant F111T/L112F, Lys-Pro-Pro-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsinvariant F111T/L112F/E287M | Sus scrofa | Lys-Pro-Ile-Gln-Phe-(p-nitrophenylalanine)-Arg-Leu | 97.7 | 0.034 | 2.87 × 10³ | pH 3.5, 25 °C | Open record: pepsin variant F111T/L112F/E287M, Lys-Pro-Ile-Gln-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsinvariant F111T/L112F/E287M | Sus scrofa | Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | 97.8 | 0.018 | 5.43 × 10³ | pH 3.5, 25 °C | Open record: pepsin variant F111T/L112F/E287M, Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsinvariant F111T/L112F/E287M | Sus scrofa | Lys-Pro-Ile-Leu-Phe-(p-nitrophenylalanine)-Arg-Leu | 60.4 | 0.0062 | 1.007 × 10⁴ | pH 3.5, 25 °C | Open record: pepsin variant F111T/L112F/E287M, Lys-Pro-Ile-Leu-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsinvariant F111T/L112F/E287M | Sus scrofa | Lys-Pro-Ile-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu | 41.4 | 0.075 | 550 | pH 3.5, 25 °C | Open record: pepsin variant F111T/L112F/E287M, Lys-Pro-Ile-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsinvariant F111T/L112F/E287M | Sus scrofa | Lys-Pro-Pro-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | 79.8 | 0.024 | 3.32 × 10³ | pH 3.5, 25 °C | Open record: pepsin variant F111T/L112F/E287M, Lys-Pro-Pro-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsinvariant F111T/L112F/T222V/E287M | Sus scrofa | Lys-Pro-Ile-Gln-Phe-(p-nitrophenylalanine)-Arg-Leu | 68.9 | 0.015 | 4.59 × 10³ | pH 3.5, 25 °C | Open record: pepsin variant F111T/L112F/T222V/E287M, Lys-Pro-Ile-Gln-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsinvariant F111T/L112F/T222V/E287M | Sus scrofa | Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | 52.3 | 0.011 | 4.75 × 10³ | pH 3.5, 25 °C | Open record: pepsin variant F111T/L112F/T222V/E287M, Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsinvariant F111T/L112F/T222V/E287M | Sus scrofa | Lys-Pro-Ile-Leu-Phe-(p-nitrophenylalanine)-Arg-Leu | 36.3 | 0.0064 | 6.05 × 10³ | pH 3.5, 25 °C | Open record: pepsin variant F111T/L112F/T222V/E287M, Lys-Pro-Ile-Leu-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsinvariant F111T/L112F/T222V/E287M | Sus scrofa | Lys-Pro-Ile-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu | 32.3 | 0.03 | 1.08 × 10³ | pH 3.5, 25 °C | Open record: pepsin variant F111T/L112F/T222V/E287M, Lys-Pro-Ile-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsinvariant F111T/L112F/T222V/E287M | Sus scrofa | Lys-Pro-Pro-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | 58.9 | 0.013 | 4.53 × 10³ | pH 3.5, 25 °C | Open record: pepsin variant F111T/L112F/T222V/E287M, Lys-Pro-Pro-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsinvariant T222V | Sus scrofa | Lys-Pro-Ile-Gln-Phe-(p-nitrophenylalanine)-Arg-Leu | 49.7 | 0.02 | 2.49 × 10³ | pH 3.5, 25 °C | Open record: pepsin variant T222V, Lys-Pro-Ile-Gln-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsinvariant T222V | Sus scrofa | Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | 41.5 | 0.02 | 2.08 × 10³ | pH 3.5, 25 °C | Open record: pepsin variant T222V, Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsinvariant T222V | Sus scrofa | Lys-Pro-Ile-Leu-Phe-(p-nitrophenylalanine)-Arg-Leu | 22.1 | 0.0051 | 4.42 × 10³ | pH 3.5, 25 °C | Open record: pepsin variant T222V, Lys-Pro-Ile-Leu-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsinvariant T222V | Sus scrofa | Lys-Pro-Ile-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu | 40.5 | 0.085 | 480 | pH 3.5, 25 °C | Open record: pepsin variant T222V, Lys-Pro-Ile-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsinvariant T222V | Sus scrofa | Lys-Pro-Pro-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | 71.4 | 0.042 | 1.7 × 10³ | pH 3.5, 25 °C | Open record: pepsin variant T222V, Lys-Pro-Pro-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsinvariant T222V/E287M | Sus scrofa | Lys-Pro-Ile-Gln-Phe-(p-nitrophenylalanine)-Arg-Leu | 77.4 | 0.036 | 2.15 × 10³ | pH 3.5, 25 °C | Open record: pepsin variant T222V/E287M, Lys-Pro-Ile-Gln-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsinvariant T222V/E287M | Sus scrofa | Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | 56.8 | 0.011 | 5.16 × 10³ | pH 3.5, 25 °C | Open record: pepsin variant T222V/E287M, Lys-Pro-Ile-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsinvariant T222V/E287M | Sus scrofa | Lys-Pro-Ile-Leu-Phe-(p-nitrophenylalanine)-Arg-Leu | 35.6 | 0.0052 | 7.12 × 10³ | pH 3.5, 25 °C | Open record: pepsin variant T222V/E287M, Lys-Pro-Ile-Leu-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsinvariant T222V/E287M | Sus scrofa | Lys-Pro-Ile-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu | 55.5 | 0.134 | 410 | pH 3.5, 25 °C | Open record: pepsin variant T222V/E287M, Lys-Pro-Ile-Lys-Phe-(p-nitrophenylalanine)-Arg-Leu |
| pepsinvariant T222V/E287M | Sus scrofa | Lys-Pro-Pro-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu | 70 | 0.027 | 2.59 × 10³ | pH 3.5, 25 °C | Open record: pepsin variant T222V/E287M, Lys-Pro-Pro-Glu-Phe-(p-nitrophenylalanine)-Arg-Leu |