Values CatLog read from this paper
Glutamate 325 is a general acid-base catalyst in the reaction catalyzed by fructose-2,6-bisphosphatase
PMID 11123954doi:10.1021/bi0020170Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 6 records (10 values) from this paper: fructose-6-phosphate,2-kinase:fructose-2,6-bisphosphatase (rat testis isozyme RT2K), Fru 2,6-Pase activity from Rattus norvegicus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| fructose-6-phosphate,2-kinase:fructose-2,6-bisphosphatase (rat testis isozyme RT2K), Fru 2,6-Pase activityvariant E325A | Rattus norvegicus | fructose 2,6-bisphosphateF26P2 0.02-500 µM | 0.0019 | 0.0091 | 0.21 | pH 7, 25 °C | Open record: fructose-6-phosphate,2-kinase:fructose-2,6-bisphosphatase (rat testis isozyme RT2K), Fru 2,6-Pase activity variant E325A, fructose 2,6-bisphosphate |
| fructose-6-phosphate,2-kinase:fructose-2,6-bisphosphatase (rat testis isozyme RT2K), Fru 2,6-Pase activityvariant E325A | Rattus norvegicus | fructose 2,6-bisphosphate | 0.0033 | — | — | 25 °C | Open record: fructose-6-phosphate,2-kinase:fructose-2,6-bisphosphatase (rat testis isozyme RT2K), Fru 2,6-Pase activity variant E325A, fructose 2,6-bisphosphate |
| fructose-6-phosphate,2-kinase:fructose-2,6-bisphosphatase (rat testis isozyme RT2K), Fru 2,6-Pase activityvariant E325A | Rattus norvegicus | fructose 2,6-bisphosphatewith 50 mM formate (chemical rescue) | 0.0087 | — | — | 25 °C | Open record: fructose-6-phosphate,2-kinase:fructose-2,6-bisphosphatase (rat testis isozyme RT2K), Fru 2,6-Pase activity variant E325A, fructose 2,6-bisphosphate |
| fructose-6-phosphate,2-kinase:fructose-2,6-bisphosphatase (rat testis isozyme RT2K), Fru 2,6-Pase activityvariant E325A | Rattus norvegicus | fructose 2,6-bisphosphatewith saturating formate (chemical rescue) | — | 4 × 10⁻⁴ | — | pH 7, 25 °C | Open record: fructose-6-phosphate,2-kinase:fructose-2,6-bisphosphatase (rat testis isozyme RT2K), Fru 2,6-Pase activity variant E325A, fructose 2,6-bisphosphate |
| fructose-6-phosphate,2-kinase:fructose-2,6-bisphosphatase (rat testis isozyme RT2K), Fru 2,6-Pase activityvariant Wo (Trp-less: all four Trp replaced by Phe) | Rattus norvegicus | fructose 2,6-bisphosphate | 0.042 | 1.2 × 10⁻⁴ | 353 | pH 7, 25 °C | Open record: fructose-6-phosphate,2-kinase:fructose-2,6-bisphosphatase (rat testis isozyme RT2K), Fru 2,6-Pase activity variant Wo (Trp-less: all four Trp replaced by Phe), fructose 2,6-bisphosphate |
| fructose-6-phosphate,2-kinase:fructose-2,6-bisphosphatase (rat testis isozyme RT2K), Fru 2,6-Pase activityvariant Wo (Trp-less: all four Trp replaced by Phe) | Rattus norvegicus | fructose 2,6-bisphosphate | 0.052 | — | — | 25 °C | Open record: fructose-6-phosphate,2-kinase:fructose-2,6-bisphosphatase (rat testis isozyme RT2K), Fru 2,6-Pase activity variant Wo (Trp-less: all four Trp replaced by Phe), fructose 2,6-bisphosphate |