Values CatLog read from this paper
A GTP-dependent vertebrate-type phosphoenolpyruvate carboxykinase from Mycobacterium smegmatis
PMID 11278451doi:10.1074/jbc.m008960200Open in Browse
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CatLog read 13 records (23 values) from this paper: phosphoenolpyruvate carboxykinase (GTP-dependent PCK, MsmPCK) from Mycobacterium smegmatis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| phosphoenolpyruvate carboxykinase (GTP-dependent PCK, MsmPCK) | Mycobacterium smegmatis | bicarbonate | — | 8.291 | — | pH 7.2, 37 °C | Open record: phosphoenolpyruvate carboxykinase (GTP-dependent PCK, MsmPCK), bicarbonate |
| phosphoenolpyruvate carboxykinase (GTP-dependent PCK, MsmPCK) | Mycobacterium smegmatis | guanosine 5'-diphosphate | — | 0.0656 | 473 | pH 7.2, 37 °C | Open record: phosphoenolpyruvate carboxykinase (GTP-dependent PCK, MsmPCK), guanosine 5'-diphosphate |
| phosphoenolpyruvate carboxykinase (GTP-dependent PCK, MsmPCK) | Mycobacterium smegmatis | guanosine 5'-triphosphate | — | 0.0132 | 922 | pH 7.2, 37 °C | Open record: phosphoenolpyruvate carboxykinase (GTP-dependent PCK, MsmPCK), guanosine 5'-triphosphate |
| phosphoenolpyruvate carboxykinase (GTP-dependent PCK, MsmPCK) | Mycobacterium smegmatis | inosine 5'-diphosphate | — | 0.2924 | 82 | pH 7.2, 37 °C | Open record: phosphoenolpyruvate carboxykinase (GTP-dependent PCK, MsmPCK), inosine 5'-diphosphate |
| phosphoenolpyruvate carboxykinase (GTP-dependent PCK, MsmPCK) | Mycobacterium smegmatis | oxaloacetate | — | 0.0118 | 1373 | pH 7.2, 37 °C | Open record: phosphoenolpyruvate carboxykinase (GTP-dependent PCK, MsmPCK), oxaloacetate |
| phosphoenolpyruvate carboxykinase (GTP-dependent PCK, MsmPCK) | Mycobacterium smegmatis | phosphoenolpyruvate2 mM GDP (replacing IDP); 0.1 mM MnCl2; 37 mM DTT | — | 0.4066 | 87 | pH 7.2, 37 °C | Open record: phosphoenolpyruvate carboxykinase (GTP-dependent PCK, MsmPCK), phosphoenolpyruvate |
| phosphoenolpyruvate carboxykinase (GTP-dependent PCK, MsmPCK) | Mycobacterium smegmatis | phosphoenolpyruvate2 mM GDP (replacing IDP); 0.1 mM MnCl2; no DTT | — | 0.3354 | 52 | pH 7.2, 37 °C | Open record: phosphoenolpyruvate carboxykinase (GTP-dependent PCK, MsmPCK), phosphoenolpyruvate |
| phosphoenolpyruvate carboxykinase (GTP-dependent PCK, MsmPCK) | Mycobacterium smegmatis | phosphoenolpyruvate2 mM GDP (replacing IDP); 5 µM CoCl2; no MnCl2; no DTT | — | 0.1465 | 81 | pH 7.2, 37 °C | Open record: phosphoenolpyruvate carboxykinase (GTP-dependent PCK, MsmPCK), phosphoenolpyruvate |
| phosphoenolpyruvate carboxykinase (GTP-dependent PCK, MsmPCK) | Mycobacterium smegmatis | phosphoenolpyruvate2 mM IDP; 0.1 mM MnCl2; 37 mM DTT; cosubstrate IDP | — | 0.2452 | 113 | pH 7.2, 37 °C | Open record: phosphoenolpyruvate carboxykinase (GTP-dependent PCK, MsmPCK), phosphoenolpyruvate |
| phosphoenolpyruvate carboxykinase (GTP-dependent PCK, MsmPCK) | Mycobacterium smegmatis | phosphoenolpyruvate2 mM IDP; 0.1 mM MnCl2; no DTT; cosubstrate IDP | — | 0.1617 | 66 | pH 7.2, 37 °C | Open record: phosphoenolpyruvate carboxykinase (GTP-dependent PCK, MsmPCK), phosphoenolpyruvate |
| phosphoenolpyruvate carboxykinase (GTP-dependent PCK, MsmPCK) | Mycobacterium smegmatis | phosphoenolpyruvate2 mM IDP; 5 µM CoCl2; no MnCl2; no DTT; cosubstrate IDP | — | 0.1025 | 76 | pH 7.2, 37 °C | Open record: phosphoenolpyruvate carboxykinase (GTP-dependent PCK, MsmPCK), phosphoenolpyruvate |
| phosphoenolpyruvate carboxykinase (GTP-dependent PCK, MsmPCK) | Mycobacterium smegmatis | phosphoenolpyruvate2 mM IDP; 0.1 mM MnCl2; 37 mM DTT; 3.5; cosubstrate IDP | — | 0.26 | — | pH 7.2, 37 °C | Open record: phosphoenolpyruvate carboxykinase (GTP-dependent PCK, MsmPCK), phosphoenolpyruvate |
| phosphoenolpyruvate carboxykinase (GTP-dependent PCK, MsmPCK) | Mycobacterium smegmatis | phosphoenolpyruvate2 mM IDP; 0.1 mM MnCl2; 37 mM DTT; 0.125; cosubstrate IDP | — | 0.26 | — | pH 7.2, 37 °C | Open record: phosphoenolpyruvate carboxykinase (GTP-dependent PCK, MsmPCK), phosphoenolpyruvate |