Values CatLog read from this paper
4-Hydroxyacetophenone monooxygenase from Pseudomonas fluorescens ACB. A novel flavoprotein catalyzing Baeyer-Villiger oxidation of aromatic compounds
PMID 11322873doi:10.1046/j.1432-1327.2001.02137.xOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 12 records (34 values) from this paper: 4-hydroxyacetophenone monooxygenase (HAPMO) from Pseudomonas fluorescens.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 2 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| 4-hydroxyacetophenone monooxygenase (HAPMO) | Pseudomonas fluorescens | 4-Aminoacetophenone | 12.3 | 0.003 | 4.1 × 10³ | pH 8, 30 °C | Open record: 4-hydroxyacetophenone monooxygenase (HAPMO), 4-Aminoacetophenone |
| 4-hydroxyacetophenone monooxygenase (HAPMO) | Pseudomonas fluorescens | 4-Fluoroacetophenone | 0.6 | 1.04 | 0.6 | pH 8, 30 °C | Open record: 4-hydroxyacetophenone monooxygenase (HAPMO), 4-Fluoroacetophenone |
| 4-hydroxyacetophenone monooxygenase (HAPMO) | Pseudomonas fluorescens | 4-Hydroxy-3-methylacetophenone | 5.4 | 0.38 | 14 | pH 8, 30 °C | Open record: 4-hydroxyacetophenone monooxygenase (HAPMO), 4-Hydroxy-3-methylacetophenone |
| 4-hydroxyacetophenone monooxygenase (HAPMO) | Pseudomonas fluorescens | 4-Hydroxyacetophenone | 10.1 | 0.039 | 259 | pH 8, 30 °C | Open record: 4-hydroxyacetophenone monooxygenase (HAPMO), 4-Hydroxyacetophenone |
| 4-hydroxyacetophenone monooxygenase (HAPMO) | Pseudomonas fluorescens | 4-Hydroxybenzaldehyde | 7.6 | 0.101 | 75 | pH 8, 30 °C | Open record: 4-hydroxyacetophenone monooxygenase (HAPMO), 4-Hydroxybenzaldehyde |
| 4-hydroxyacetophenone monooxygenase (HAPMO) | Pseudomonas fluorescens | 4-Hydroxypropiophenone | 10.6 | 0.012 | 883 | pH 8, 30 °C | Open record: 4-hydroxyacetophenone monooxygenase (HAPMO), 4-Hydroxypropiophenone |
| 4-hydroxyacetophenone monooxygenase (HAPMO) | Pseudomonas fluorescens | 4-Methoxyacetophenone | 1.7 | 0.541 | 3.1 | pH 8, 30 °C | Open record: 4-hydroxyacetophenone monooxygenase (HAPMO), 4-Methoxyacetophenone |
| 4-hydroxyacetophenone monooxygenase (HAPMO) | Pseudomonas fluorescens | 4-Methylacetophenone | 6.3 | 0.161 | 39 | pH 8, 30 °C | Open record: 4-hydroxyacetophenone monooxygenase (HAPMO), 4-Methylacetophenone |
| 4-hydroxyacetophenone monooxygenase (HAPMO) | Pseudomonas fluorescens | acetophenone | 4.5 | 2.27 | 2 | pH 8, 30 °C | Open record: 4-hydroxyacetophenone monooxygenase (HAPMO), acetophenone |
| 4-hydroxyacetophenone monooxygenase (HAPMO) | Pseudomonas fluorescens | NADPHair-saturated 50 mM potassium phosphate buffer | 9.3 | 0.064 | 145 | pH 8, 30 °C | Open record: 4-hydroxyacetophenone monooxygenase (HAPMO), NADPH |
| 4-hydroxyacetophenone monooxygenase (HAPMO) | Pseudomonas fluorescens | NADPHNADPH oxidase (uncoupling) activity; no aromatic substrate | 0.11 | 0.005 | 22 calc. | pH 8, 30 °C | Open record: 4-hydroxyacetophenone monooxygenase (HAPMO), NADPH |
| 4-hydroxyacetophenone monooxygenase (HAPMO)variant G490A | Pseudomonas fluorescens | NADPH | 0.4 | 0.7 | 0.57 calc. | pH 8, 30 °C | Open record: 4-hydroxyacetophenone monooxygenase (HAPMO) variant G490A, NADPH |