CatLogenzyme kinetics catalog

Values CatLog read from this paper

Identification of the substrate specificity-conferring amino acid residues of 4-coumarate:coenzyme A ligase allows the rational design of mutant enzymes with new catalytic properties

PMID 11323416doi:10.1074/jbc.m100355200Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 22 records (22 values) from this paper: At4CL2 (4-coumarate:coenzyme A ligase isoform 2) from Arabidopsis thaliana.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 6.2.1.12

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
At4CL2 (4-coumarate:coenzyme A ligase isoform 2)Arabidopsis thalianacaffeic acid (3,4-dihydroxycinnamic acid)—0.024—Open
At4CL2 (4-coumarate:coenzyme A ligase isoform 2)variant K320AArabidopsis thalianacaffeic acid (3,4-dihydroxycinnamic acid)—0.055—Open
At4CL2 (4-coumarate:coenzyme A ligase isoform 2)variant K320AArabidopsis thalianaferulic acid (4-hydroxy-3-methoxycinnamic acid)—0.04—Open
At4CL2 (4-coumarate:coenzyme A ligase isoform 2)variant K320A/M293PArabidopsis thalianacaffeic acid (3,4-dihydroxycinnamic acid)—0.084—Open
At4CL2 (4-coumarate:coenzyme A ligase isoform 2)variant K320A/M293PArabidopsis thalianaferulic acid (4-hydroxy-3-methoxycinnamic acid)—0.058—Open
At4CL2 (4-coumarate:coenzyme A ligase isoform 2)variant K320LArabidopsis thalianacaffeic acid (3,4-dihydroxycinnamic acid)—0.074—Open
At4CL2 (4-coumarate:coenzyme A ligase isoform 2)variant K320LArabidopsis thalianaferulic acid (4-hydroxy-3-methoxycinnamic acid)—0.151—Open
At4CL2 (4-coumarate:coenzyme A ligase isoform 2)variant K320L/M293PArabidopsis thalianacaffeic acid (3,4-dihydroxycinnamic acid)—0.048—Open
At4CL2 (4-coumarate:coenzyme A ligase isoform 2)variant K320L/M293PArabidopsis thalianaferulic acid (4-hydroxy-3-methoxycinnamic acid)—0.046—Open
At4CL2 (4-coumarate:coenzyme A ligase isoform 2)variant M293AArabidopsis thalianacaffeic acid (3,4-dihydroxycinnamic acid)—0.024—Open
At4CL2 (4-coumarate:coenzyme A ligase isoform 2)variant M293AArabidopsis thalianaferulic acid (4-hydroxy-3-methoxycinnamic acid)—0.558—Open
At4CL2 (4-coumarate:coenzyme A ligase isoform 2)variant M293A/V294LArabidopsis thalianacaffeic acid (3,4-dihydroxycinnamic acid)—0.024—Open
At4CL2 (4-coumarate:coenzyme A ligase isoform 2)variant M293A/V294LArabidopsis thalianaferulic acid (4-hydroxy-3-methoxycinnamic acid)—0.162—Open
At4CL2 (4-coumarate:coenzyme A ligase isoform 2)variant M293FArabidopsis thalianacaffeic acid (3,4-dihydroxycinnamic acid)—0.063—Open
At4CL2 (4-coumarate:coenzyme A ligase isoform 2)variant M293PArabidopsis thalianacaffeic acid (3,4-dihydroxycinnamic acid)—0.025—Open
At4CL2 (4-coumarate:coenzyme A ligase isoform 2)variant M293PArabidopsis thalianaferulic acid (4-hydroxy-3-methoxycinnamic acid)—0.224—Open
At4CL2 (4-coumarate:coenzyme A ligase isoform 2)variant M293P/V294MArabidopsis thalianacaffeic acid (3,4-dihydroxycinnamic acid)—0.021—Open
At4CL2 (4-coumarate:coenzyme A ligase isoform 2)variant M293P/V294MArabidopsis thalianaferulic acid (4-hydroxy-3-methoxycinnamic acid)—0.105—Open
At4CL2 (4-coumarate:coenzyme A ligase isoform 2)variant M293WArabidopsis thalianacaffeic acid (3,4-dihydroxycinnamic acid)—0.18—Open
At4CL2 (4-coumarate:coenzyme A ligase isoform 2)variant M293YArabidopsis thalianacaffeic acid (3,4-dihydroxycinnamic acid)—0.224—Open
At4CL2 (4-coumarate:coenzyme A ligase isoform 2)variant V294LArabidopsis thalianacaffeic acid (3,4-dihydroxycinnamic acid)—0.023—Open
At4CL2 (4-coumarate:coenzyme A ligase isoform 2)variant V294MArabidopsis thalianacaffeic acid (3,4-dihydroxycinnamic acid)—0.022—Open