Values CatLog read from this paper
Mechanism of the family 1 beta-glucosidase from Streptomyces sp: catalytic residues and kinetic studies
PMID 11352732doi:10.1021/bi002947jOpen in Browse
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CatLog read 37 records (111 values) from this paper: Bgl3 beta-glucosidase from Streptomyces sp..
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| Bgl3 beta-glucosidase | Streptomyces sp. | 2,3-dinitrophenyl beta-D-glucopyranoside | 11.7 | 0.015 | 770 | pH 6.5, 30 °C | Open record: Bgl3 beta-glucosidase, 2,3-dinitrophenyl beta-D-glucopyranoside |
| Bgl3 beta-glucosidase | Streptomyces sp. | 2,4-dinitrophenyl beta-D-glucopyranoside | 30.4 | 0.008 | 3.83 × 10³ | pH 6.5, 30 °C | Open record: Bgl3 beta-glucosidase, 2,4-dinitrophenyl beta-D-glucopyranoside |
| Bgl3 beta-glucosidase | Streptomyces sp. | 2-nitrophenyl beta-D-glucopyranoside | 7.72 | 0.023 | 343 | pH 6.5, 30 °C | Open record: Bgl3 beta-glucosidase, 2-nitrophenyl beta-D-glucopyranoside |
| Bgl3 beta-glucosidase | Streptomyces sp. | 3,4-dinitrophenyl beta-D-glucopyranoside | 18.2 | 0.064 | 287 | pH 6.5, 30 °C | Open record: Bgl3 beta-glucosidase, 3,4-dinitrophenyl beta-D-glucopyranoside |
| Bgl3 beta-glucosidase | Streptomyces sp. | 3,5-dinitrophenyl beta-D-glucopyranoside | 20.5 | 0.054 | 378 | pH 6.5, 30 °C | Open record: Bgl3 beta-glucosidase, 3,5-dinitrophenyl beta-D-glucopyranoside |
| Bgl3 beta-glucosidase | Streptomyces sp. | 3-nitrophenyl beta-D-glucopyranoside | 15.8 | 0.24 | 65.2 | pH 6.5, 30 °C | Open record: Bgl3 beta-glucosidase, 3-nitrophenyl beta-D-glucopyranoside |
| Bgl3 beta-glucosidase | Streptomyces sp. | 4-bromophenyl beta-D-glucopyranoside | 3.32 | 0.47 | 7.11 | pH 6.5, 30 °C | Open record: Bgl3 beta-glucosidase, 4-bromophenyl beta-D-glucopyranoside |
| Bgl3 beta-glucosidase | Streptomyces sp. | 4-methylumbelliferyl beta-D-glucopyranoside | 11.7 | 0.058 | 204 | pH 6.5, 30 °C | Open record: Bgl3 beta-glucosidase, 4-methylumbelliferyl beta-D-glucopyranoside |
| Bgl3 beta-glucosidase | Streptomyces sp. | 4-nitrophenyl beta-D-glucopyranoside | 28.4 | 0.15 | 190 | pH 6.5, 50 °C | Open record: Bgl3 beta-glucosidase, 4-nitrophenyl beta-D-glucopyranoside |
| Bgl3 beta-glucosidase | Streptomyces sp. | 4-nitrophenyl beta-D-glucopyranoside | 8.14 | 0.1 | 78.5 | pH 6.5, 30 °C | Open record: Bgl3 beta-glucosidase, 4-nitrophenyl beta-D-glucopyranoside |
| Bgl3 beta-glucosidase | Streptomyces sp. | cellobiose | 35.6 | 4.1 | 8.6 | pH 6.5, 50 °C | Open record: Bgl3 beta-glucosidase, cellobiose |
| Bgl3 beta-glucosidase | Streptomyces sp. | p-nitrophenyl beta-D-fucoside | 37.1 | 0.14 | 265 | pH 6.5, 50 °C | Open record: Bgl3 beta-glucosidase, p-nitrophenyl beta-D-fucoside |
| Bgl3 beta-glucosidase | Streptomyces sp. | p-nitrophenyl beta-D-galactoside | 118 | 7.3 | 16.1 | pH 6.5, 50 °C | Open record: Bgl3 beta-glucosidase, p-nitrophenyl beta-D-galactoside |
| Bgl3 beta-glucosidase | Streptomyces sp. | p-nitrophenyl beta-D-xyloside | 0.63 | 3 | 0.211 | pH 6.5, 50 °C | Open record: Bgl3 beta-glucosidase, p-nitrophenyl beta-D-xyloside |
| Bgl3 beta-glucosidase | Streptomyces sp. | phenyl beta-D-glucopyranoside | 0.66 | 2.3 | 0.283 | pH 6.5, 30 °C | Open record: Bgl3 beta-glucosidase, phenyl beta-D-glucopyranoside |
| Bgl3 beta-glucosidasevariant E178A | Streptomyces sp. | 2,4-dinitrophenyl beta-D-glucopyranosideno sodium azide (azide 0 mM) | 0.022 | 3 × 10⁻⁴ | 74 | pH 6.5, 30 °C | Open record: Bgl3 beta-glucosidase variant E178A, 2,4-dinitrophenyl beta-D-glucopyranoside |
| Bgl3 beta-glucosidasevariant E178A | Streptomyces sp. | 2,4-dinitrophenyl beta-D-glucopyranoside50 mM sodium azide (exogenous nucleophile, chemical rescue) | 4.14 | 0.0018 | 2.3 × 10³ | pH 6.5, 30 °C | Open record: Bgl3 beta-glucosidase variant E178A, 2,4-dinitrophenyl beta-D-glucopyranoside |
| Bgl3 beta-glucosidasevariant E178A | Streptomyces sp. | 4-nitrophenyl beta-D-glucopyranoside | 0.036 | 0.65 | 0.0552 | pH 6.5, 50 °C | Open record: Bgl3 beta-glucosidase variant E178A, 4-nitrophenyl beta-D-glucopyranoside |
| Bgl3 beta-glucosidasevariant E178A | Streptomyces sp. | 4-nitrophenyl beta-D-glucopyranoside50 mM phosphate buffer; I = 0.15 M with added KCl | 0.12 | 1.436 | 0.0818 | pH 6.5, 50 °C | Open record: Bgl3 beta-glucosidase variant E178A, 4-nitrophenyl beta-D-glucopyranoside |
| Bgl3 beta-glucosidasevariant E178A | Streptomyces sp. | p-nitrophenyl beta-D-fucoside | 0.011 | 0.22 | 0.0486 | pH 6.5, 50 °C | Open record: Bgl3 beta-glucosidase variant E178A, p-nitrophenyl beta-D-fucoside |
| Bgl3 beta-glucosidasevariant E178A | Streptomyces sp. | p-nitrophenyl beta-D-galactoside | 0.004 | 0.12 | 0.0365 | pH 6.5, 50 °C | Open record: Bgl3 beta-glucosidase variant E178A, p-nitrophenyl beta-D-galactoside |
| Bgl3 beta-glucosidasevariant E178Q | Streptomyces sp. | 2,4-dinitrophenyl beta-D-glucopyranosideno sodium azide (azide 0 mM) | 0.076 | 0.0054 | 14 | pH 6.5, 30 °C | Open record: Bgl3 beta-glucosidase variant E178Q, 2,4-dinitrophenyl beta-D-glucopyranoside |
| Bgl3 beta-glucosidasevariant E178Q | Streptomyces sp. | 2,4-dinitrophenyl beta-D-glucopyranoside50 mM sodium azide (exogenous nucleophile, chemical rescue) | 2.04 | 0.0032 | 640 | pH 6.5, 30 °C | Open record: Bgl3 beta-glucosidase variant E178Q, 2,4-dinitrophenyl beta-D-glucopyranoside |
| Bgl3 beta-glucosidasevariant E178Q | Streptomyces sp. | 4-nitrophenyl beta-D-glucopyranoside | 0.015 | 0.02 | 0.777 | pH 6.5, 50 °C | Open record: Bgl3 beta-glucosidase variant E178Q, 4-nitrophenyl beta-D-glucopyranoside |
| Bgl3 beta-glucosidasevariant E178Q | Streptomyces sp. | 4-nitrophenyl beta-D-glucopyranoside50 mM phosphate buffer; I = 0.15 M with added KCl | 1.38 | 0.575 | 2.4 | pH 6.5, 50 °C | Open record: Bgl3 beta-glucosidase variant E178Q, 4-nitrophenyl beta-D-glucopyranoside |
| Bgl3 beta-glucosidasevariant E178Q | Streptomyces sp. | p-nitrophenyl beta-D-fucoside | 1.08 | 0.11 | 9.9 | pH 6.5, 50 °C | Open record: Bgl3 beta-glucosidase variant E178Q, p-nitrophenyl beta-D-fucoside |
| Bgl3 beta-glucosidasevariant E178Q | Streptomyces sp. | p-nitrophenyl beta-D-galactoside | 0.66 | 4.5 | 0.15 | pH 6.5, 50 °C | Open record: Bgl3 beta-glucosidase variant E178Q, p-nitrophenyl beta-D-galactoside |
| Bgl3 beta-glucosidasevariant E383A | Streptomyces sp. | 2,4-dinitrophenyl beta-D-glucopyranosideno sodium azide (azide 0 mM) | 3.5 × 10⁻⁴ | 0.027 | 0.013 | pH 6.5, 30 °C | Open record: Bgl3 beta-glucosidase variant E383A, 2,4-dinitrophenyl beta-D-glucopyranoside |
| Bgl3 beta-glucosidasevariant E383A | Streptomyces sp. | 2,4-dinitrophenyl beta-D-glucopyranoside255 mM sodium azide (exogenous nucleophile, chemical rescue) | 0.0031 | 0.168 | 0.019 | pH 6.5, 30 °C | Open record: Bgl3 beta-glucosidase variant E383A, 2,4-dinitrophenyl beta-D-glucopyranoside |
| Bgl3 beta-glucosidasevariant E383A | Streptomyces sp. | 2,4-dinitrophenyl beta-D-glucopyranoside | 0.047 | 0.99 | 0.048 | pH 6.5, 30 °C | Open record: Bgl3 beta-glucosidase variant E383A, 2,4-dinitrophenyl beta-D-glucopyranoside |
| Bgl3 beta-glucosidasevariant E383A | Streptomyces sp. | 4-nitrophenyl beta-D-glucopyranoside | 4 × 10⁻⁵ | 0.03 | 0.0014 | pH 7, 30 °C | Open record: Bgl3 beta-glucosidase variant E383A, 4-nitrophenyl beta-D-glucopyranoside |
| Bgl3 beta-glucosidasevariant E383A | Streptomyces sp. | 4-nitrophenyl beta-D-glucopyranoside50 mM phosphate buffer; I = 0.15 M with added KCl | 4 × 10⁻⁵ | 0.036 | 0.0011 | pH 7, 30 °C | Open record: Bgl3 beta-glucosidase variant E383A, 4-nitrophenyl beta-D-glucopyranoside |
| Bgl3 beta-glucosidasevariant E383A | Streptomyces sp. | p-nitrophenyl beta-D-fucoside | 2 × 10⁻⁴ | 0.23 | 9.2 × 10⁻⁴ | pH 7, 30 °C | Open record: Bgl3 beta-glucosidase variant E383A, p-nitrophenyl beta-D-fucoside |
| Bgl3 beta-glucosidasevariant E383A | Streptomyces sp. | p-nitrophenyl beta-D-galactoside | 8 × 10⁻⁴ | 0.04 | 0.02 | pH 7, 30 °C | Open record: Bgl3 beta-glucosidase variant E383A, p-nitrophenyl beta-D-galactoside |
| Bgl3 beta-glucosidasevariant E383Q | Streptomyces sp. | 4-nitrophenyl beta-D-glucopyranoside | 3 × 10⁻⁵ | 0.2 | 1.6 × 10⁻⁴ | pH 7, 30 °C | Open record: Bgl3 beta-glucosidase variant E383Q, 4-nitrophenyl beta-D-glucopyranoside |
| Bgl3 beta-glucosidasevariant E383Q | Streptomyces sp. | p-nitrophenyl beta-D-fucoside | 2 × 10⁻⁵ | 0.26 | 6 × 10⁻⁵ | pH 7, 30 °C | Open record: Bgl3 beta-glucosidase variant E383Q, p-nitrophenyl beta-D-fucoside |
| Bgl3 beta-glucosidasevariant E383Q | Streptomyces sp. | p-nitrophenyl beta-D-galactoside | 1 × 10⁻⁴ | 0.04 | 0.004 | pH 7, 30 °C | Open record: Bgl3 beta-glucosidase variant E383Q, p-nitrophenyl beta-D-galactoside |