Values CatLog read from this paper
Residues at the active site of the esterase 2 from Alicyclobacillus acidocaldarius involved in substrate specificity and catalytic activity at high temperature
PMID 11447219doi:10.1074/jbc.m103017200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 43 records (129 values) from this paper: esterase 2 (EST2) from Alicyclobacillus acidocaldarius.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| esterase 2 (EST2) | Alicyclobacillus acidocaldarius | 4-nitrophenyl butanoate | 2.18 × 10³ | 0.76 | 3 × 10³ | pH 7.1, 70 °C | Open record: esterase 2 (EST2), 4-nitrophenyl butanoate |
| esterase 2 (EST2) | Alicyclobacillus acidocaldarius | 4-nitrophenyl decanoate | 950 | 0.018 | 5.2 × 10⁴ | pH 7.1, 70 °C | Open record: esterase 2 (EST2), 4-nitrophenyl decanoate |
| esterase 2 (EST2) | Alicyclobacillus acidocaldarius | 4-nitrophenyl dodecanoate | 390 | 0.019 | 2.1 × 10⁴ | pH 7.1, 70 °C | Open record: esterase 2 (EST2), 4-nitrophenyl dodecanoate |
| esterase 2 (EST2) | Alicyclobacillus acidocaldarius | 4-nitrophenyl hexadecanoate | 21 | 0.042 | 500 | pH 7.1, 70 °C | Open record: esterase 2 (EST2), 4-nitrophenyl hexadecanoate |
| esterase 2 (EST2) | Alicyclobacillus acidocaldarius | 4-nitrophenyl hexanoate | 3.42 × 10³ | 0.22 | 1.5 × 10⁴ | pH 7.1, 70 °C | Open record: esterase 2 (EST2), 4-nitrophenyl hexanoate |
| esterase 2 (EST2) | Alicyclobacillus acidocaldarius | 4-nitrophenyl octanoate | 1.96 × 10³ | 0.028 | 6.9 × 10⁴ | pH 7.1, 70 °C | Open record: esterase 2 (EST2), 4-nitrophenyl octanoate |
| esterase 2 (EST2) | Alicyclobacillus acidocaldarius | 4-nitrophenyl tetradecanoate | 105 | 0.031 | 3 × 10³ | pH 7.1, 70 °C | Open record: esterase 2 (EST2), 4-nitrophenyl tetradecanoate |
| esterase 2 (EST2)variant M211S | Alicyclobacillus acidocaldarius | 4-nitrophenyl butanoate | 4.23 × 10³ | 1.77 | 2.4 × 10³ | pH 7.1, 70 °C | Open record: esterase 2 (EST2) variant M211S, 4-nitrophenyl butanoate |
| esterase 2 (EST2)variant M211S | Alicyclobacillus acidocaldarius | 4-nitrophenyl decanoate | 860 | 0.008 | 1.07 × 10⁵ | pH 7.1, 70 °C | Open record: esterase 2 (EST2) variant M211S, 4-nitrophenyl decanoate |
| esterase 2 (EST2)variant M211S | Alicyclobacillus acidocaldarius | 4-nitrophenyl dodecanoate | 350 | 0.01 | 3.5 × 10⁴ | pH 7.1, 70 °C | Open record: esterase 2 (EST2) variant M211S, 4-nitrophenyl dodecanoate |
| esterase 2 (EST2)variant M211S | Alicyclobacillus acidocaldarius | 4-nitrophenyl hexadecanoate | 15.6 | 0.0205 | 800 | pH 7.1, 70 °C | Open record: esterase 2 (EST2) variant M211S, 4-nitrophenyl hexadecanoate |
| esterase 2 (EST2)variant M211S | Alicyclobacillus acidocaldarius | 4-nitrophenyl hexanoate | 3.89 × 10³ | 0.41 | 9 × 10³ | pH 7.1, 70 °C | Open record: esterase 2 (EST2) variant M211S, 4-nitrophenyl hexanoate |
| esterase 2 (EST2)variant M211S | Alicyclobacillus acidocaldarius | 4-nitrophenyl octanoate | 1855 | 0.037 | 5 × 10⁴ | pH 7.1, 70 °C | Open record: esterase 2 (EST2) variant M211S, 4-nitrophenyl octanoate |
| esterase 2 (EST2)variant M211S | Alicyclobacillus acidocaldarius | 4-nitrophenyl tetradecanoate | 105 | 0.017 | 6 × 10³ | pH 7.1, 70 °C | Open record: esterase 2 (EST2) variant M211S, 4-nitrophenyl tetradecanoate |
| esterase 2 (EST2)variant M211S/R215L | Alicyclobacillus acidocaldarius | 4-nitrophenyl butanoate | 2.75 × 10³ | 1.06 | 3 × 10³ | pH 7.1, 70 °C | Open record: esterase 2 (EST2) variant M211S/R215L, 4-nitrophenyl butanoate |
| esterase 2 (EST2)variant M211S/R215L | Alicyclobacillus acidocaldarius | 4-nitrophenyl decanoate | 970 | 0.008 | 1.16 × 10⁵ | pH 7.1, 70 °C | Open record: esterase 2 (EST2) variant M211S/R215L, 4-nitrophenyl decanoate |
| esterase 2 (EST2)variant M211S/R215L | Alicyclobacillus acidocaldarius | 4-nitrophenyl dodecanoate | 940 | 0.008 | 1.2 × 10⁵ | pH 7.1, 70 °C | Open record: esterase 2 (EST2) variant M211S/R215L, 4-nitrophenyl dodecanoate |
| esterase 2 (EST2)variant M211S/R215L | Alicyclobacillus acidocaldarius | 4-nitrophenyl hexadecanoate | 44 | 0.034 | 1.3 × 10³ | pH 7.1, 70 °C | Open record: esterase 2 (EST2) variant M211S/R215L, 4-nitrophenyl hexadecanoate |
| esterase 2 (EST2)variant M211S/R215L | Alicyclobacillus acidocaldarius | 4-nitrophenyl hexanoate | 2.3 × 10³ | 0.099 | 2.3 × 10⁴ | pH 7.1, 70 °C | Open record: esterase 2 (EST2) variant M211S/R215L, 4-nitrophenyl hexanoate |
| esterase 2 (EST2)variant M211S/R215L | Alicyclobacillus acidocaldarius | 4-nitrophenyl octanoate | 1645 | 0.022 | 7.5 × 10⁴ | pH 7.1, 70 °C | Open record: esterase 2 (EST2) variant M211S/R215L, 4-nitrophenyl octanoate |
| esterase 2 (EST2)variant M211S/R215L | Alicyclobacillus acidocaldarius | 4-nitrophenyl tetradecanoate | 235 | 0.022 | 1.1 × 10⁴ | pH 7.1, 70 °C | Open record: esterase 2 (EST2) variant M211S/R215L, 4-nitrophenyl tetradecanoate |
| esterase 2 (EST2)variant M211T | Alicyclobacillus acidocaldarius | 4-nitrophenyl butanoate0.09% (w/v) gum arabic; 7.5% propan-2-ol | 5.24 × 10³ | 1.86 | 3 × 10³ | pH 7.1, 70 °C | Open record: esterase 2 (EST2) variant M211T, 4-nitrophenyl butanoate |
| esterase 2 (EST2)variant M211T | Alicyclobacillus acidocaldarius | 4-nitrophenyl butanoateassay without propan-2-ol (Table I footnote a) | 3.04 × 10³ | 0.073 | 4.2 × 10⁴ | pH 7.1, 70 °C | Open record: esterase 2 (EST2) variant M211T, 4-nitrophenyl butanoate |
| esterase 2 (EST2)variant M211T | Alicyclobacillus acidocaldarius | 4-nitrophenyl decanoate | 626 | 0.019 | 3.3 × 10⁴ | pH 7.1, 70 °C | Open record: esterase 2 (EST2) variant M211T, 4-nitrophenyl decanoate |
| esterase 2 (EST2)variant M211T | Alicyclobacillus acidocaldarius | 4-nitrophenyl dodecanoate | 110 | 0.007 | 1.6 × 10⁴ | pH 7.1, 70 °C | Open record: esterase 2 (EST2) variant M211T, 4-nitrophenyl dodecanoate |
| esterase 2 (EST2)variant M211T | Alicyclobacillus acidocaldarius | 4-nitrophenyl hexadecanoate | 8.3 | 0.023 | 400 | pH 7.1, 70 °C | Open record: esterase 2 (EST2) variant M211T, 4-nitrophenyl hexadecanoate |
| esterase 2 (EST2)variant M211T | Alicyclobacillus acidocaldarius | 4-nitrophenyl hexanoate | 4.44 × 10³ | 0.51 | 9 × 10³ | pH 7.1, 70 °C | Open record: esterase 2 (EST2) variant M211T, 4-nitrophenyl hexanoate |
| esterase 2 (EST2)variant M211T | Alicyclobacillus acidocaldarius | 4-nitrophenyl octanoate | 1.74 × 10³ | 0.1 | 1.7 × 10⁴ | pH 7.1, 70 °C | Open record: esterase 2 (EST2) variant M211T, 4-nitrophenyl octanoate |
| esterase 2 (EST2)variant M211T | Alicyclobacillus acidocaldarius | 4-nitrophenyl tetradecanoate | 40 | 0.02 | 2 × 10³ | pH 7.1, 70 °C | Open record: esterase 2 (EST2) variant M211T, 4-nitrophenyl tetradecanoate |
| esterase 2 (EST2)variant M211V | Alicyclobacillus acidocaldarius | 4-nitrophenyl butanoate | 4 × 10³ | 1.27 | 3 × 10³ | pH 7.1, 70 °C | Open record: esterase 2 (EST2) variant M211V, 4-nitrophenyl butanoate |
| esterase 2 (EST2)variant M211V | Alicyclobacillus acidocaldarius | 4-nitrophenyl decanoate | 465 | 0.021 | 2.2 × 10⁴ | pH 7.1, 70 °C | Open record: esterase 2 (EST2) variant M211V, 4-nitrophenyl decanoate |
| esterase 2 (EST2)variant M211V | Alicyclobacillus acidocaldarius | 4-nitrophenyl dodecanoate | 112 | 0.015 | 7 × 10³ | pH 7.1, 70 °C | Open record: esterase 2 (EST2) variant M211V, 4-nitrophenyl dodecanoate |
| esterase 2 (EST2)variant M211V | Alicyclobacillus acidocaldarius | 4-nitrophenyl hexadecanoate | 7 | 0.026 | 300 | pH 7.1, 70 °C | Open record: esterase 2 (EST2) variant M211V, 4-nitrophenyl hexadecanoate |
| esterase 2 (EST2)variant M211V | Alicyclobacillus acidocaldarius | 4-nitrophenyl hexanoate | 1.5 × 10³ | 0.21 | 7 × 10³ | pH 7.1, 70 °C | Open record: esterase 2 (EST2) variant M211V, 4-nitrophenyl hexanoate |
| esterase 2 (EST2)variant M211V | Alicyclobacillus acidocaldarius | 4-nitrophenyl octanoate | 1376 | 0.06 | 2.3 × 10⁴ | pH 7.1, 70 °C | Open record: esterase 2 (EST2) variant M211V, 4-nitrophenyl octanoate |
| esterase 2 (EST2)variant M211V | Alicyclobacillus acidocaldarius | 4-nitrophenyl tetradecanoate | 27 | 0.019 | 1.4 × 10³ | pH 7.1, 70 °C | Open record: esterase 2 (EST2) variant M211V, 4-nitrophenyl tetradecanoate |
| esterase 2 (EST2)variant R215L | Alicyclobacillus acidocaldarius | 4-nitrophenyl butanoate | 3.2 × 10³ | 0.75 | 4 × 10³ | pH 7.1, 70 °C | Open record: esterase 2 (EST2) variant R215L, 4-nitrophenyl butanoate |
| esterase 2 (EST2)variant R215L | Alicyclobacillus acidocaldarius | 4-nitrophenyl decanoate | 1.67 × 10³ | 0.011 | 1.58 × 10⁵ | pH 7.1, 70 °C | Open record: esterase 2 (EST2) variant R215L, 4-nitrophenyl decanoate |
| esterase 2 (EST2)variant R215L | Alicyclobacillus acidocaldarius | 4-nitrophenyl dodecanoate | 705 | 0.021 | 3.4 × 10⁴ | pH 7.1, 70 °C | Open record: esterase 2 (EST2) variant R215L, 4-nitrophenyl dodecanoate |
| esterase 2 (EST2)variant R215L | Alicyclobacillus acidocaldarius | 4-nitrophenyl hexadecanoate | 35 | 0.031 | 1.1 × 10³ | pH 7.1, 70 °C | Open record: esterase 2 (EST2) variant R215L, 4-nitrophenyl hexadecanoate |
| esterase 2 (EST2)variant R215L | Alicyclobacillus acidocaldarius | 4-nitrophenyl hexanoate | 3.66 × 10³ | 0.09 | 4.1 × 10⁴ | pH 7.1, 70 °C | Open record: esterase 2 (EST2) variant R215L, 4-nitrophenyl hexanoate |
| esterase 2 (EST2)variant R215L | Alicyclobacillus acidocaldarius | 4-nitrophenyl octanoate | 3.08 × 10³ | 0.025 | 1.26 × 10⁵ | pH 7.1, 70 °C | Open record: esterase 2 (EST2) variant R215L, 4-nitrophenyl octanoate |
| esterase 2 (EST2)variant R215L | Alicyclobacillus acidocaldarius | 4-nitrophenyl tetradecanoate | 232 | 0.022 | 1.05 × 10⁴ | pH 7.1, 70 °C | Open record: esterase 2 (EST2) variant R215L, 4-nitrophenyl tetradecanoate |