Values CatLog read from this paper
Evolution of enzymatic activities in the enolase superfamily: identification of the general acid catalyst in the active site of D-glucarate dehydratase from Escherichia coli
PMID 11513584doi:10.1021/bi010733bOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 9 records (18 values) from this paper: D-glucarate dehydratase (GlucD) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| D-glucarate dehydratase (GlucD) | Escherichia coli | 4-deoxy-4-fluoro-D-glucarate | 44 | — | 13 | pH 7.5, 22 °C | Open record: D-glucarate dehydratase (GlucD), 4-deoxy-4-fluoro-D-glucarate |
| D-glucarate dehydratase (GlucD)variant H339Q | Escherichia coli | 4-deoxy-4-fluoro-D-glucarate | 0.03 | — | 0.03 | pH 7.5, 22 °C | Open record: D-glucarate dehydratase (GlucD) variant H339Q, 4-deoxy-4-fluoro-D-glucarate |
| D-glucarate dehydratase (GlucD)variant K207R | Escherichia coli | 4-deoxy-4-fluoro-D-glucarate | 0.002 | — | 0.0013 | pH 7.5, 22 °C | Open record: D-glucarate dehydratase (GlucD) variant K207R, 4-deoxy-4-fluoro-D-glucarate |
| D-glucarate dehydratase (GlucD)variant N341D | Escherichia coli | 4-deoxy-4-fluoro-D-glucarate | 0.28 | — | 0.19 | pH 7.5, 22 °C | Open record: D-glucarate dehydratase (GlucD) variant N341D, 4-deoxy-4-fluoro-D-glucarate |
| D-glucarate dehydratase (GlucD)variant N341D | Escherichia coli | D-glucarate | 0.022 | — | 0.1 | pH 7.5, 22 °C | Open record: D-glucarate dehydratase (GlucD) variant N341D, D-glucarate |
| D-glucarate dehydratase (GlucD)variant N341D | Escherichia coli | L-idarate | 0.099 | — | 0.062 | pH 7.5, 22 °C | Open record: D-glucarate dehydratase (GlucD) variant N341D, L-idarate |
| D-glucarate dehydratase (GlucD)variant N341L | Escherichia coli | 4-deoxy-4-fluoro-D-glucarate | 2.7 | — | 11.5 | pH 7.5, 22 °C | Open record: D-glucarate dehydratase (GlucD) variant N341L, 4-deoxy-4-fluoro-D-glucarate |
| D-glucarate dehydratase (GlucD)variant N341L | Escherichia coli | D-glucarate | 0.004 | — | 0.027 | pH 7.5, 22 °C | Open record: D-glucarate dehydratase (GlucD) variant N341L, D-glucarate |
| D-glucarate dehydratase (GlucD)variant N341L | Escherichia coli | L-idarate | 0.036 | — | 0.045 | pH 7.5, 22 °C | Open record: D-glucarate dehydratase (GlucD) variant N341L, L-idarate |