Values CatLog read from this paper
Effects of flavin-binding motif amino acid mutations in the NADH-cytochrome b5 reductase catalytic domain on protein stability and catalysis
PMID 11574067doi:10.1093/oxfordjournals.jbchem.a003010Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 22 records (66 values) from this paper: NADH-cytochrome b5 reductase catalytic domain (Pb5R) from Sus scrofa.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| NADH-cytochrome b5 reductase catalytic domain (Pb5R) | Sus scrofa | NADH | 1.1 × 10³ | 0.0031 | 3.54 × 10⁵ | pH 7, 25 °C | Open record: NADH-cytochrome b5 reductase catalytic domain (Pb5R), NADH |
| NADH-cytochrome b5 reductase catalytic domain (Pb5R) | Sus scrofa | trypsin-solubilized form of porcine liver cytochrome b5 | 661 | 0.0104 | 6.36 × 10⁴ | pH 7, 25 °C | Open record: NADH-cytochrome b5 reductase catalytic domain (Pb5R), trypsin-solubilized form of porcine liver cytochrome b5 |
| NADH-cytochrome b5 reductase catalytic domain (Pb5R)variant K97A | Sus scrofa | NADH | 1.06 × 10³ | 0.0044 | 2.4 × 10⁵ | pH 7, 25 °C | Open record: NADH-cytochrome b5 reductase catalytic domain (Pb5R) variant K97A, NADH |
| NADH-cytochrome b5 reductase catalytic domain (Pb5R)variant K97A | Sus scrofa | trypsin-solubilized form of porcine liver cytochrome b5 | 191 | 0.0161 | 1.19 × 10⁴ | pH 7, 25 °C | Open record: NADH-cytochrome b5 reductase catalytic domain (Pb5R) variant K97A, trypsin-solubilized form of porcine liver cytochrome b5 |
| NADH-cytochrome b5 reductase catalytic domain (Pb5R)variant K97R | Sus scrofa | NADH | 909 | 0.0029 | 3.13 × 10⁵ | pH 7, 25 °C | Open record: NADH-cytochrome b5 reductase catalytic domain (Pb5R) variant K97R, NADH |
| NADH-cytochrome b5 reductase catalytic domain (Pb5R)variant K97R | Sus scrofa | trypsin-solubilized form of porcine liver cytochrome b5 | 415 | 0.0069 | 6.01 × 10⁴ | pH 7, 25 °C | Open record: NADH-cytochrome b5 reductase catalytic domain (Pb5R) variant K97R, trypsin-solubilized form of porcine liver cytochrome b5 |
| NADH-cytochrome b5 reductase catalytic domain (Pb5R)variant R63A | Sus scrofa | NADH | 403 | 0.104 | 3.9 × 10³ | pH 7, 25 °C | Open record: NADH-cytochrome b5 reductase catalytic domain (Pb5R) variant R63A, NADH |
| NADH-cytochrome b5 reductase catalytic domain (Pb5R)variant R63A | Sus scrofa | trypsin-solubilized form of porcine liver cytochrome b5 | 121 | 0.0421 | 2.9 × 10³ | pH 7, 25 °C | Open record: NADH-cytochrome b5 reductase catalytic domain (Pb5R) variant R63A, trypsin-solubilized form of porcine liver cytochrome b5 |
| NADH-cytochrome b5 reductase catalytic domain (Pb5R)variant R63K | Sus scrofa | NADH | 515 | 0.0021 | 2.45 × 10⁵ | pH 7, 25 °C | Open record: NADH-cytochrome b5 reductase catalytic domain (Pb5R) variant R63K, NADH |
| NADH-cytochrome b5 reductase catalytic domain (Pb5R)variant R63K | Sus scrofa | trypsin-solubilized form of porcine liver cytochrome b5 | 347 | 0.0073 | 4.75 × 10⁴ | pH 7, 25 °C | Open record: NADH-cytochrome b5 reductase catalytic domain (Pb5R) variant R63K, trypsin-solubilized form of porcine liver cytochrome b5 |
| NADH-cytochrome b5 reductase catalytic domain (Pb5R)variant R63Q | Sus scrofa | NADH | 412 | 0.021 | 1.96 × 10⁴ | pH 7, 25 °C | Open record: NADH-cytochrome b5 reductase catalytic domain (Pb5R) variant R63Q, NADH |
| NADH-cytochrome b5 reductase catalytic domain (Pb5R)variant R63Q | Sus scrofa | trypsin-solubilized form of porcine liver cytochrome b5 | 105 | 0.0185 | 5.7 × 10³ | pH 7, 25 °C | Open record: NADH-cytochrome b5 reductase catalytic domain (Pb5R) variant R63Q, trypsin-solubilized form of porcine liver cytochrome b5 |
| NADH-cytochrome b5 reductase catalytic domain (Pb5R)variant S99A | Sus scrofa | NADH | 435 | 0.026 | 1.67 × 10⁴ | pH 7, 25 °C | Open record: NADH-cytochrome b5 reductase catalytic domain (Pb5R) variant S99A, NADH |
| NADH-cytochrome b5 reductase catalytic domain (Pb5R)variant S99A | Sus scrofa | trypsin-solubilized form of porcine liver cytochrome b5 | 295 | 0.0148 | 1.99 × 10⁴ | pH 7, 25 °C | Open record: NADH-cytochrome b5 reductase catalytic domain (Pb5R) variant S99A, trypsin-solubilized form of porcine liver cytochrome b5 |
| NADH-cytochrome b5 reductase catalytic domain (Pb5R)variant S99T | Sus scrofa | NADH | 851 | 0.0019 | 4.47 × 10⁵ | pH 7, 25 °C | Open record: NADH-cytochrome b5 reductase catalytic domain (Pb5R) variant S99T, NADH |
| NADH-cytochrome b5 reductase catalytic domain (Pb5R)variant S99T | Sus scrofa | trypsin-solubilized form of porcine liver cytochrome b5 | 520 | 0.0118 | 4.41 × 10⁴ | pH 7, 25 °C | Open record: NADH-cytochrome b5 reductase catalytic domain (Pb5R) variant S99T, trypsin-solubilized form of porcine liver cytochrome b5 |
| NADH-cytochrome b5 reductase catalytic domain (Pb5R)variant S99V | Sus scrofa | NADH | 375 | 0.0094 | 3.95 × 10⁴ | pH 7, 25 °C | Open record: NADH-cytochrome b5 reductase catalytic domain (Pb5R) variant S99V, NADH |
| NADH-cytochrome b5 reductase catalytic domain (Pb5R)variant S99V | Sus scrofa | trypsin-solubilized form of porcine liver cytochrome b5 | 165 | 0.0099 | 1.67 × 10⁴ | pH 7, 25 °C | Open record: NADH-cytochrome b5 reductase catalytic domain (Pb5R) variant S99V, trypsin-solubilized form of porcine liver cytochrome b5 |
| NADH-cytochrome b5 reductase catalytic domain (Pb5R)variant Y65A | Sus scrofa | NADH | 180 | 0.0042 | 4.29 × 10⁴ | pH 7, 25 °C | Open record: NADH-cytochrome b5 reductase catalytic domain (Pb5R) variant Y65A, NADH |
| NADH-cytochrome b5 reductase catalytic domain (Pb5R)variant Y65A | Sus scrofa | trypsin-solubilized form of porcine liver cytochrome b5 | 107 | 0.005 | 2.14 × 10⁴ | pH 7, 25 °C | Open record: NADH-cytochrome b5 reductase catalytic domain (Pb5R) variant Y65A, trypsin-solubilized form of porcine liver cytochrome b5 |
| NADH-cytochrome b5 reductase catalytic domain (Pb5R)variant Y65F | Sus scrofa | NADH | 501 | 0.003 | 1.67 × 10⁵ | pH 7, 25 °C | Open record: NADH-cytochrome b5 reductase catalytic domain (Pb5R) variant Y65F, NADH |
| NADH-cytochrome b5 reductase catalytic domain (Pb5R)variant Y65F | Sus scrofa | trypsin-solubilized form of porcine liver cytochrome b5 | 375 | 0.0062 | 6.05 × 10⁴ | pH 7, 25 °C | Open record: NADH-cytochrome b5 reductase catalytic domain (Pb5R) variant Y65F, trypsin-solubilized form of porcine liver cytochrome b5 |