Values CatLog read from this paper
Conversion of a glutamate dehydrogenase into methionine/norleucine dehydrogenase by site-directed mutagenesis
PMID 11722565doi:10.1046/j.0014-2956.2001.02523.xOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 36 records (68 values) from this paper: glutamate dehydrogenase (GDH) from Clostridium symbiosum.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| glutamate dehydrogenase (GDH) | Clostridium symbiosum | 2-oxoglutarate | 179 | 0.22 | 814 | pH 7, 25 °C | Open record: glutamate dehydrogenase (GDH), 2-oxoglutarate |
| glutamate dehydrogenase (GDH) | Clostridium symbiosum | L-glutamate | 15.2 | 3.04 | 5 | pH 7, 25 °C | Open record: glutamate dehydrogenase (GDH), L-glutamate |
| glutamate dehydrogenase (GDH) | Clostridium symbiosum | L-glutamate | 20 | 4.2 | 4.8 | pH 8, 25 °C | Open record: glutamate dehydrogenase (GDH), L-glutamate |
| glutamate dehydrogenase (GDH) | Clostridium symbiosum | L-norleucine | — | — | 9 × 10⁻⁶ | pH 8, 25 °C | Open record: glutamate dehydrogenase (GDH), L-norleucine |
| glutamate dehydrogenase (GDH) | Clostridium symbiosum | NAD+ | — | 0.113 | — | pH 7, 25 °C | Open record: glutamate dehydrogenase (GDH), NAD+ |
| glutamate dehydrogenase (GDH)variant A163G | Clostridium symbiosum | 2-oxoglutarate | 210 | 1.1 | 194 | pH 7, 25 °C | Open record: glutamate dehydrogenase (GDH) variant A163G, 2-oxoglutarate |
| glutamate dehydrogenase (GDH)variant A163G | Clostridium symbiosum | L-glutamate | 0.9 | 19 | 0.0474 | pH 7, 25 °C | Open record: glutamate dehydrogenase (GDH) variant A163G, L-glutamate |
| glutamate dehydrogenase (GDH)variant A163G | Clostridium symbiosum | L-glutamate | 11 | 2 | 5.5 | pH 8, 25 °C | Open record: glutamate dehydrogenase (GDH) variant A163G, L-glutamate |
| glutamate dehydrogenase (GDH)variant A163G | Clostridium symbiosum | L-methionine | — | — | 0.0023 | pH 8, 25 °C | Open record: glutamate dehydrogenase (GDH) variant A163G, L-methionine |
| glutamate dehydrogenase (GDH)variant A163G | Clostridium symbiosum | L-norleucine | — | — | 0.0015 | pH 8, 25 °C | Open record: glutamate dehydrogenase (GDH) variant A163G, L-norleucine |
| glutamate dehydrogenase (GDH)variant A163G | Clostridium symbiosum | NAD+ | — | 0.0626 | — | pH 7, 25 °C | Open record: glutamate dehydrogenase (GDH) variant A163G, NAD+ |
| glutamate dehydrogenase (GDH)variant K89L | Clostridium symbiosum | 2-oxo-γ-methiolbutyric acid | 8.82 | 53.5 | 0.165 | pH 7, 25 °C | Open record: glutamate dehydrogenase (GDH) variant K89L, 2-oxo-γ-methiolbutyric acid |
| glutamate dehydrogenase (GDH)variant K89L | Clostridium symbiosum | 2-oxocaproic acid | 13.8 | 39.4 | 0.35 | pH 7, 25 °C | Open record: glutamate dehydrogenase (GDH) variant K89L, 2-oxocaproic acid |
| glutamate dehydrogenase (GDH)variant K89L | Clostridium symbiosum | L-methionine | — | — | 7 × 10⁻⁵ | pH 7, 25 °C | Open record: glutamate dehydrogenase (GDH) variant K89L, L-methionine |
| glutamate dehydrogenase (GDH)variant K89L | Clostridium symbiosum | L-methionine | — | — | 4 × 10⁻⁵ | pH 8, 25 °C | Open record: glutamate dehydrogenase (GDH) variant K89L, L-methionine |
| glutamate dehydrogenase (GDH)variant K89L | Clostridium symbiosum | L-norleucine | — | — | 1.6 × 10⁻⁴ | pH 7, 25 °C | Open record: glutamate dehydrogenase (GDH) variant K89L, L-norleucine |
| glutamate dehydrogenase (GDH)variant K89L | Clostridium symbiosum | L-norleucine | — | — | 6 × 10⁻⁶ | pH 8, 25 °C | Open record: glutamate dehydrogenase (GDH) variant K89L, L-norleucine |
| glutamate dehydrogenase (GDH)variant K89L/A163G | Clostridium symbiosum | 2-oxo-γ-methiolbutyric acid | 9.6 | 6.6 | 1.45 | pH 7, 25 °C | Open record: glutamate dehydrogenase (GDH) variant K89L/A163G, 2-oxo-γ-methiolbutyric acid |
| glutamate dehydrogenase (GDH)variant K89L/A163G | Clostridium symbiosum | 2-oxocaproic acid | 13.9 | 5.97 | 2.33 | pH 7, 25 °C | Open record: glutamate dehydrogenase (GDH) variant K89L/A163G, 2-oxocaproic acid |
| glutamate dehydrogenase (GDH)variant K89L/A163G | Clostridium symbiosum | 2-oxoglutarate | 2.95 | 6.74 | 0.44 | pH 7, 25 °C | Open record: glutamate dehydrogenase (GDH) variant K89L/A163G, 2-oxoglutarate |
| glutamate dehydrogenase (GDH)variant K89L/A163G | Clostridium symbiosum | L-glutamate | — | — | 1.1 × 10⁻⁴ | pH 7, 25 °C | Open record: glutamate dehydrogenase (GDH) variant K89L/A163G, L-glutamate |
| glutamate dehydrogenase (GDH)variant K89L/A163G | Clostridium symbiosum | L-glutamate | — | — | 4.5 × 10⁻⁴ | pH 8, 25 °C | Open record: glutamate dehydrogenase (GDH) variant K89L/A163G, L-glutamate |
| glutamate dehydrogenase (GDH)variant K89L/A163G | Clostridium symbiosum | L-methionine | — | — | 3.8 × 10⁻⁴ | pH 7, 25 °C | Open record: glutamate dehydrogenase (GDH) variant K89L/A163G, L-methionine |
| glutamate dehydrogenase (GDH)variant K89L/A163G | Clostridium symbiosum | L-methionine | — | — | 0.0126 | pH 8, 25 °C | Open record: glutamate dehydrogenase (GDH) variant K89L/A163G, L-methionine |
| glutamate dehydrogenase (GDH)variant K89L/A163G | Clostridium symbiosum | L-norleucine | — | — | 8.2 × 10⁻⁴ | pH 7, 25 °C | Open record: glutamate dehydrogenase (GDH) variant K89L/A163G, L-norleucine |
| glutamate dehydrogenase (GDH)variant K89L/A163G | Clostridium symbiosum | L-norleucine | — | — | 0.0163 | pH 8, 25 °C | Open record: glutamate dehydrogenase (GDH) variant K89L/A163G, L-norleucine |
| glutamate dehydrogenase (GDH)variant K89L/S380A/A163G | Clostridium symbiosum | 2-oxo-γ-methiolbutyric acid | 6.6 | 5.9 | 1.12 | pH 7, 25 °C | Open record: glutamate dehydrogenase (GDH) variant K89L/S380A/A163G, 2-oxo-γ-methiolbutyric acid |
| glutamate dehydrogenase (GDH)variant K89L/S380A/A163G | Clostridium symbiosum | 2-oxocaproic acid | 11.2 | 5.5 | 2.04 | pH 7, 25 °C | Open record: glutamate dehydrogenase (GDH) variant K89L/S380A/A163G, 2-oxocaproic acid |
| glutamate dehydrogenase (GDH)variant K89L/S380A/A163G | Clostridium symbiosum | L-methionine | — | — | 8.2 × 10⁻⁴ | pH 7, 25 °C | Open record: glutamate dehydrogenase (GDH) variant K89L/S380A/A163G, L-methionine |
| glutamate dehydrogenase (GDH)variant K89L/S380A/A163G | Clostridium symbiosum | L-methionine | — | — | 0.0078 | pH 8, 25 °C | Open record: glutamate dehydrogenase (GDH) variant K89L/S380A/A163G, L-methionine |
| glutamate dehydrogenase (GDH)variant K89L/S380A/A163G | Clostridium symbiosum | L-norleucine | — | — | 9.8 × 10⁻⁴ | pH 7, 25 °C | Open record: glutamate dehydrogenase (GDH) variant K89L/S380A/A163G, L-norleucine |
| glutamate dehydrogenase (GDH)variant K89L/S380A/A163G | Clostridium symbiosum | L-norleucine | — | — | 0.0176 | pH 8, 25 °C | Open record: glutamate dehydrogenase (GDH) variant K89L/S380A/A163G, L-norleucine |
| glutamate dehydrogenase (GDH)variant S380A | Clostridium symbiosum | 2-oxoglutarate | 38.3 | 1.7 | 23 | pH 7, 25 °C | Open record: glutamate dehydrogenase (GDH) variant S380A, 2-oxoglutarate |
| glutamate dehydrogenase (GDH)variant S380A | Clostridium symbiosum | L-glutamate | 1.6 | 91.7 | 0.0175 | pH 7, 25 °C | Open record: glutamate dehydrogenase (GDH) variant S380A, L-glutamate |
| glutamate dehydrogenase (GDH)variant S380A | Clostridium symbiosum | L-glutamate | 31.4 | 261 | 0.1203 | pH 8, 25 °C | Open record: glutamate dehydrogenase (GDH) variant S380A, L-glutamate |
| glutamate dehydrogenase (GDH)variant S380A | Clostridium symbiosum | NAD+ | — | 0.603 | — | pH 7, 25 °C | Open record: glutamate dehydrogenase (GDH) variant S380A, NAD+ |