Values CatLog read from this paper

A functional role for a flexible loop containing Glu182 in the class II fructose-1,6-bisphosphate aldolase from Escherichia coli

PMID 11779234doi:10.1006/jmbi.2001.5237Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 18 records (36 values) from this paper: class II fructose-1,6-bisphosphate aldolase from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 18 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 4.1.2.13

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
class II fructose-1,6-bisphosphate aldolase	Escherichia coli	[1(S)-2H]dihydroxyacetone phosphate	2.72	0.05	54 calc.	pH 8, 30 °C	Open record: class II fructose-1,6-bisphosphate aldolase, [1(S)-2H]dihydroxyacetone phosphate
class II fructose-1,6-bisphosphate aldolase	Escherichia coli	D-fructose 1,6-bisphosphate	10.5	0.17	62 calc.	30 °C	Open record: class II fructose-1,6-bisphosphate aldolase, D-fructose 1,6-bisphosphate
class II fructose-1,6-bisphosphate aldolase	Escherichia coli	D-fructose 1,6-bisphosphate	1.83	0.02	92 calc.	pH 8, 30 °C	Open record: class II fructose-1,6-bisphosphate aldolase, D-fructose 1,6-bisphosphate
class II fructose-1,6-bisphosphate aldolase	Escherichia coli	dihydroxyacetone phosphate	2.83	0.03	94 calc.	pH 8, 30 °C	Open record: class II fructose-1,6-bisphosphate aldolase, dihydroxyacetone phosphate
class II fructose-1,6-bisphosphate aldolasevariant E174A	Escherichia coli	D-fructose 1,6-bisphosphate	0.013	0.13	0.10 calc.	30 °C	Open record: class II fructose-1,6-bisphosphate aldolase variant E174A, D-fructose 1,6-bisphosphate
class II fructose-1,6-bisphosphate aldolasevariant E174A	Escherichia coli	D-fructose 1,6-bisphosphate	0.033	0.02	1.7 calc.	pH 8, 30 °C	Open record: class II fructose-1,6-bisphosphate aldolase variant E174A, D-fructose 1,6-bisphosphate
class II fructose-1,6-bisphosphate aldolasevariant E174A	Escherichia coli	dihydroxyacetone phosphate	0.033	0.02	1.7 calc.	pH 8, 30 °C	Open record: class II fructose-1,6-bisphosphate aldolase variant E174A, dihydroxyacetone phosphate
class II fructose-1,6-bisphosphate aldolasevariant E181A	Escherichia coli	D-fructose 1,6-bisphosphate	5.83	0.3	19 calc.	30 °C	Open record: class II fructose-1,6-bisphosphate aldolase variant E181A, D-fructose 1,6-bisphosphate
class II fructose-1,6-bisphosphate aldolasevariant E181A	Escherichia coli	D-fructose 1,6-bisphosphate	1.2	0.08	15 calc.	pH 8, 30 °C	Open record: class II fructose-1,6-bisphosphate aldolase variant E181A, D-fructose 1,6-bisphosphate
class II fructose-1,6-bisphosphate aldolasevariant E181A	Escherichia coli	dihydroxyacetone phosphate	4.5	0.35	13 calc.	pH 8, 30 °C	Open record: class II fructose-1,6-bisphosphate aldolase variant E181A, dihydroxyacetone phosphate
class II fructose-1,6-bisphosphate aldolasevariant E182A	Escherichia coli	[1(S)-2H]dihydroxyacetone phosphate	0.0083	0.11	0.076 calc.	pH 8, 30 °C	Open record: class II fructose-1,6-bisphosphate aldolase variant E182A, [1(S)-2H]dihydroxyacetone phosphate
class II fructose-1,6-bisphosphate aldolasevariant E182A	Escherichia coli	D-fructose 1,6-bisphosphate	0.033	0.02	1.7 calc.	30 °C	Open record: class II fructose-1,6-bisphosphate aldolase variant E182A, D-fructose 1,6-bisphosphate
class II fructose-1,6-bisphosphate aldolasevariant E182A	Escherichia coli	D-fructose 1,6-bisphosphate	5.67	0.5	11 calc.	pH 8, 30 °C	Open record: class II fructose-1,6-bisphosphate aldolase variant E182A, D-fructose 1,6-bisphosphate
class II fructose-1,6-bisphosphate aldolasevariant E182A	Escherichia coli	dihydroxyacetone phosphate	0.05	0.14	0.36 calc.	pH 8, 30 °C	Open record: class II fructose-1,6-bisphosphate aldolase variant E182A, dihydroxyacetone phosphate
class II fructose-1,6-bisphosphate aldolasevariant G176A/G179A/G180A/G184A	Escherichia coli	[1(S)-2H]dihydroxyacetone phosphate	8.3 × 10⁻⁴	0.17	0.0049 calc.	pH 8, 30 °C	Open record: class II fructose-1,6-bisphosphate aldolase variant G176A/G179A/G180A/G184A, [1(S)-2H]dihydroxyacetone phosphate
class II fructose-1,6-bisphosphate aldolasevariant G176A/G179A/G180A/G184A	Escherichia coli	D-fructose 1,6-bisphosphate	0.12	0.07	1.7 calc.	30 °C	Open record: class II fructose-1,6-bisphosphate aldolase variant G176A/G179A/G180A/G184A, D-fructose 1,6-bisphosphate
class II fructose-1,6-bisphosphate aldolasevariant G176A/G179A/G180A/G184A	Escherichia coli	D-fructose 1,6-bisphosphate	0.57	0.72	0.79 calc.	pH 8, 30 °C	Open record: class II fructose-1,6-bisphosphate aldolase variant G176A/G179A/G180A/G184A, D-fructose 1,6-bisphosphate
class II fructose-1,6-bisphosphate aldolasevariant G176A/G179A/G180A/G184A	Escherichia coli	dihydroxyacetone phosphate	0.002	0.16	0.013 calc.	pH 8, 30 °C	Open record: class II fructose-1,6-bisphosphate aldolase variant G176A/G179A/G180A/G184A, dihydroxyacetone phosphate