Values CatLog read from this paper
Two glutamate residues, Glu 208 alpha and Glu 197 beta, are crucial for phosphorylation and dephosphorylation of the active-site histidine residue in succinyl-CoA synthetase
PMID 11781092doi:10.1021/bi011518yOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 16 records (24 values) from this paper: succinyl-CoA synthetase from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 8 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| succinyl-CoA synthetasevariant E197D (β-subunit, E197βD) | Escherichia coli | adenosine 5'-triphosphate | 29.58 | 0.137 | 216 calc. | pH 7.4, 22 °C | Open record: succinyl-CoA synthetase variant E197D (β-subunit, E197βD), adenosine 5'-triphosphate |
| succinyl-CoA synthetasevariant E197D (β-subunit, E197βD) | Escherichia coli | coenzyme A | — | 0.0027 | — | pH 7.4, 22 °C | Open record: succinyl-CoA synthetase variant E197D (β-subunit, E197βD), coenzyme A |
| succinyl-CoA synthetasevariant E197D (β-subunit, E197βD) | Escherichia coli | guanosine 5'-triphosphate | 29.58 | 0.311 | 95.1 calc. | pH 7.4, 22 °C | Open record: succinyl-CoA synthetase variant E197D (β-subunit, E197βD), guanosine 5'-triphosphate |
| succinyl-CoA synthetasevariant E197D (β-subunit, E197βD) | Escherichia coli | succinate | — | 0.87 | — | pH 7.4, 22 °C | Open record: succinyl-CoA synthetase variant E197D (β-subunit, E197βD), succinate |
| succinyl-CoA synthetasevariant E197Q (β-subunit, E197βQ) | Escherichia coli | adenosine 5'-triphosphate | 0.0142 | 0.106 | 0.130 calc. | pH 7.4, 22 °C | Open record: succinyl-CoA synthetase variant E197Q (β-subunit, E197βQ), adenosine 5'-triphosphate |
| succinyl-CoA synthetasevariant E197Q (β-subunit, E197βQ) | Escherichia coli | coenzyme A | — | 0.0018 | — | pH 7.4, 22 °C | Open record: succinyl-CoA synthetase variant E197Q (β-subunit, E197βQ), coenzyme A |
| succinyl-CoA synthetasevariant E197Q (β-subunit, E197βQ) | Escherichia coli | guanosine 5'-triphosphate | 0.0035 | 0.02 | 0.17 calc. | pH 7.4, 22 °C | Open record: succinyl-CoA synthetase variant E197Q (β-subunit, E197βQ), guanosine 5'-triphosphate |
| succinyl-CoA synthetasevariant E197Q (β-subunit, E197βQ) | Escherichia coli | succinate | — | 0.4 | — | pH 7.4, 22 °C | Open record: succinyl-CoA synthetase variant E197Q (β-subunit, E197βQ), succinate |
| succinyl-CoA synthetasevariant E208D (α-subunit, E208αD) | Escherichia coli | adenosine 5'-triphosphate | 39.05 | 0.116 | 337 calc. | pH 7.4, 22 °C | Open record: succinyl-CoA synthetase variant E208D (α-subunit, E208αD), adenosine 5'-triphosphate |
| succinyl-CoA synthetasevariant E208D (α-subunit, E208αD) | Escherichia coli | coenzyme A | — | 0.002 | — | pH 7.4, 22 °C | Open record: succinyl-CoA synthetase variant E208D (α-subunit, E208αD), coenzyme A |
| succinyl-CoA synthetasevariant E208D (α-subunit, E208αD) | Escherichia coli | guanosine 5'-triphosphate | 8.993 | 0.063 | 140 calc. | pH 7.4, 22 °C | Open record: succinyl-CoA synthetase variant E208D (α-subunit, E208αD), guanosine 5'-triphosphate |
| succinyl-CoA synthetasevariant E208D (α-subunit, E208αD) | Escherichia coli | succinate | — | 0.26 | — | pH 7.4, 22 °C | Open record: succinyl-CoA synthetase variant E208D (α-subunit, E208αD), succinate |
| succinyl-CoA synthetasevariant E208Q (α-subunit, E208αQ) | Escherichia coli | adenosine 5'-triphosphate | 0.0095 | 0.041 | 0.23 calc. | pH 7.4, 22 °C | Open record: succinyl-CoA synthetase variant E208Q (α-subunit, E208αQ), adenosine 5'-triphosphate |
| succinyl-CoA synthetasevariant E208Q (α-subunit, E208αQ) | Escherichia coli | coenzyme A | — | 0.0013 | — | pH 7.4, 22 °C | Open record: succinyl-CoA synthetase variant E208Q (α-subunit, E208αQ), coenzyme A |
| succinyl-CoA synthetasevariant E208Q (α-subunit, E208αQ) | Escherichia coli | guanosine 5'-triphosphate | 0.0047 | 0.011 | 0.42 calc. | pH 7.4, 22 °C | Open record: succinyl-CoA synthetase variant E208Q (α-subunit, E208αQ), guanosine 5'-triphosphate |
| succinyl-CoA synthetasevariant E208Q (α-subunit, E208αQ) | Escherichia coli | succinate | — | 0.27 | — | pH 7.4, 22 °C | Open record: succinyl-CoA synthetase variant E208Q (α-subunit, E208αQ), succinate |