Values CatLog read from this paper

Cold adaptation of xylose isomerase from Thermus thermophilus through random PCR mutagenesis. Gene cloning and protein characterization

PMID 11784309doi:10.1046/j.0014-2956.2002.02631.xOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 8 records (24 values) from this paper: xylose isomerase from Thermus thermophilus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 5.3.1.5

Records read from this paper
EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsOpen record
xylose isomeraseThermus thermophilusD-glucose16.3146.80.11pH 7, 60 °COpen record: xylose isomerase, D-glucose
xylose isomeraseThermus thermophilusD-xylose46.63.4413.6pH 7, 60 °COpen record: xylose isomerase, D-xylose
xylose isomerasevariant E372GThermus thermophilusD-glucose88.7171.80.47pH 7, 60 °COpen record: xylose isomerase variant E372G, D-glucose
xylose isomerasevariant E372GThermus thermophilusD-xylose412.428.714.4pH 7, 60 °COpen record: xylose isomerase variant E372G, D-xylose
xylose isomerasevariant E372G/F163LThermus thermophilusD-glucose66.9130.80.51pH 7, 60 °COpen record: xylose isomerase variant E372G/F163L, D-glucose
xylose isomerasevariant E372G/F163LThermus thermophilusD-xylose381.689.44.3pH 7, 60 °COpen record: xylose isomerase variant E372G/F163L, D-xylose
xylose isomerasevariant E372G/V379AThermus thermophilusD-glucose39.9520.77pH 7, 60 °COpen record: xylose isomerase variant E372G/V379A, D-glucose
xylose isomerasevariant E372G/V379AThermus thermophilusD-xylose257.525.110.3pH 7, 60 °COpen record: xylose isomerase variant E372G/V379A, D-xylose