Values CatLog read from this paper

Probing substrate binding to metallo-beta-lactamase L1 from Stenotrophomonas maltophilia by using site-directed mutagenesis

PMID 11876827doi:10.1186/1471-2091-3-4Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 89 records (266 values) from this paper: metallo-β-lactamase L1 from Stenotrophomonas maltophilia.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.

EC 3.5.2.6

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
metallo-β-lactamase L1	Stenotrophomonas maltophilia	benzylpenicillin	600	0.038	1.6 × 10⁴	pH 7, 25 °C	Open record: metallo-β-lactamase L1, benzylpenicillin
metallo-β-lactamase L1	Stenotrophomonas maltophilia	biapenem	134	0.032	4.2 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1, biapenem
metallo-β-lactamase L1	Stenotrophomonas maltophilia	cefaclor	42	0.013	3.2 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1, cefaclor
metallo-β-lactamase L1	Stenotrophomonas maltophilia	cefoxitin	1.9	0.0011	1.7 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1, cefoxitin
metallo-β-lactamase L1	Stenotrophomonas maltophilia	cephalothin	82	0.0089	9.2 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1, cephalothin
metallo-β-lactamase L1	Stenotrophomonas maltophilia	imipenem	370	0.057	6.5 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1, imipenem
metallo-β-lactamase L1	Stenotrophomonas maltophilia	meropenem	157	0.015	1 × 10⁴	pH 7, 25 °C	Open record: metallo-β-lactamase L1, meropenem
metallo-β-lactamase L1	Stenotrophomonas maltophilia	nitrocefin	38	0.012	3.2 × 10³ calc.	pH 7, 25 °C	Open record: metallo-β-lactamase L1, nitrocefin
metallo-β-lactamase L1variant F158A	Stenotrophomonas maltophilia	ampicillin	270	0.165	1.6 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant F158A, ampicillin
metallo-β-lactamase L1variant F158A	Stenotrophomonas maltophilia	benzylpenicillin	138	0.05	2.8 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant F158A, benzylpenicillin
metallo-β-lactamase L1variant F158A	Stenotrophomonas maltophilia	biapenem	70	0.05	1.4 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant F158A, biapenem
metallo-β-lactamase L1variant F158A	Stenotrophomonas maltophilia	cefaclor	99	0.135	730	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant F158A, cefaclor
metallo-β-lactamase L1variant F158A	Stenotrophomonas maltophilia	cefoxitin	16	0.011	1.5 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant F158A, cefoxitin
metallo-β-lactamase L1variant F158A	Stenotrophomonas maltophilia	cephalothin	353	0.063	5.6 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant F158A, cephalothin
metallo-β-lactamase L1variant F158A	Stenotrophomonas maltophilia	imipenem	370	0.1	3.7 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant F158A, imipenem
metallo-β-lactamase L1variant F158A	Stenotrophomonas maltophilia	meropenem	36	0.007	5.1 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant F158A, meropenem
metallo-β-lactamase L1variant F158A	Stenotrophomonas maltophilia	nitrocefin	1.29 × 10³	0.123	1 × 10⁴	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant F158A, nitrocefin
metallo-β-lactamase L1variant I164A	Stenotrophomonas maltophilia	ampicillin	524	0.043	1.2 × 10⁴	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant I164A, ampicillin
metallo-β-lactamase L1variant I164A	Stenotrophomonas maltophilia	benzylpenicillin	698	0.06	1.2 × 10⁴	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant I164A, benzylpenicillin
metallo-β-lactamase L1variant I164A	Stenotrophomonas maltophilia	biapenem	112	0.055	2 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant I164A, biapenem
metallo-β-lactamase L1variant I164A	Stenotrophomonas maltophilia	cefaclor	30	0.0033	9.1 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant I164A, cefaclor
metallo-β-lactamase L1variant I164A	Stenotrophomonas maltophilia	cefoxitin	8	0.011	730	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant I164A, cefoxitin
metallo-β-lactamase L1variant I164A	Stenotrophomonas maltophilia	cephalothin	146	0.016	9.1 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant I164A, cephalothin
metallo-β-lactamase L1variant I164A	Stenotrophomonas maltophilia	imipenem	570	0.092	6.2 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant I164A, imipenem
metallo-β-lactamase L1variant I164A	Stenotrophomonas maltophilia	meropenem	96	0.014	6.9 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant I164A, meropenem
metallo-β-lactamase L1variant I164A	Stenotrophomonas maltophilia	nitrocefin	130	0.008	1.6 × 10⁴	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant I164A, nitrocefin
metallo-β-lactamase L1variant N233D	Stenotrophomonas maltophilia	ampicillin	621	0.117	5.3 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant N233D, ampicillin
metallo-β-lactamase L1variant N233D	Stenotrophomonas maltophilia	benzylpenicillin	440	0.06	7.3 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant N233D, benzylpenicillin
metallo-β-lactamase L1variant N233D	Stenotrophomonas maltophilia	biapenem	7	0.028	250	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant N233D, biapenem
metallo-β-lactamase L1variant N233D	Stenotrophomonas maltophilia	cefaclor	34	0.025	1.4 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant N233D, cefaclor
metallo-β-lactamase L1variant N233D	Stenotrophomonas maltophilia	cefoxitin	1.1	0.0011	1 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant N233D, cefoxitin
metallo-β-lactamase L1variant N233D	Stenotrophomonas maltophilia	cephalothin	65	0.018	3.6 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant N233D, cephalothin
metallo-β-lactamase L1variant N233D	Stenotrophomonas maltophilia	imipenem	158	0.071	2.2 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant N233D, imipenem
metallo-β-lactamase L1variant N233D	Stenotrophomonas maltophilia	meropenem	3.5	0.016	220	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant N233D, meropenem
metallo-β-lactamase L1variant N233D	Stenotrophomonas maltophilia	nitrocefin	21	0.009	2.3 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant N233D, nitrocefin
metallo-β-lactamase L1variant N233L	Stenotrophomonas maltophilia	ampicillin	508	0.09	5.6 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant N233L, ampicillin
metallo-β-lactamase L1variant N233L	Stenotrophomonas maltophilia	benzylpenicillin	184	0.033	5.6 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant N233L, benzylpenicillin
metallo-β-lactamase L1variant N233L	Stenotrophomonas maltophilia	biapenem	105	0.029	3.6 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant N233L, biapenem
metallo-β-lactamase L1variant N233L	Stenotrophomonas maltophilia	cefaclor	32	0.014	2.3 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant N233L, cefaclor
metallo-β-lactamase L1variant N233L	Stenotrophomonas maltophilia	cefoxitin	0.9	0.0044	200	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant N233L, cefoxitin
metallo-β-lactamase L1variant N233L	Stenotrophomonas maltophilia	cephalothin	51	0.008	6.4 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant N233L, cephalothin
metallo-β-lactamase L1variant N233L	Stenotrophomonas maltophilia	imipenem	250	0.036	6.9 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant N233L, imipenem
metallo-β-lactamase L1variant N233L	Stenotrophomonas maltophilia	meropenem	67	0.012	5.6 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant N233L, meropenem
metallo-β-lactamase L1variant N233L	Stenotrophomonas maltophilia	nitrocefin	62	0.007	8.9 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant N233L, nitrocefin
metallo-β-lactamase L1variant S224A	Stenotrophomonas maltophilia	ampicillin	339	0.125	2.7 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant S224A, ampicillin
metallo-β-lactamase L1variant S224A	Stenotrophomonas maltophilia	benzylpenicillin	580	0.07	8.3 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant S224A, benzylpenicillin
metallo-β-lactamase L1variant S224A	Stenotrophomonas maltophilia	biapenem	56	0.034	1.6 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant S224A, biapenem
metallo-β-lactamase L1variant S224A	Stenotrophomonas maltophilia	cefaclor	14	0.014	1 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant S224A, cefaclor
metallo-β-lactamase L1variant S224A	Stenotrophomonas maltophilia	cefoxitin	1	0.003	330	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant S224A, cefoxitin
metallo-β-lactamase L1variant S224A	Stenotrophomonas maltophilia	cephalothin	19.8	0.0036	5.5 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant S224A, cephalothin
metallo-β-lactamase L1variant S224A	Stenotrophomonas maltophilia	imipenem	100	0.029	3.4 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant S224A, imipenem
metallo-β-lactamase L1variant S224A	Stenotrophomonas maltophilia	meropenem	244	0.05	4.9 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant S224A, meropenem
metallo-β-lactamase L1variant S224A	Stenotrophomonas maltophilia	nitrocefin	48	0.007	6.9 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant S224A, nitrocefin
metallo-β-lactamase L1variant S224D	Stenotrophomonas maltophilia	ampicillin	10	1.1	9.1	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant S224D, ampicillin
metallo-β-lactamase L1variant S224D	Stenotrophomonas maltophilia	benzylpenicillin	42	1.6	26	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant S224D, benzylpenicillin
metallo-β-lactamase L1variant S224D	Stenotrophomonas maltophilia	biapenem	22	0.064	340	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant S224D, biapenem
metallo-β-lactamase L1variant S224D	Stenotrophomonas maltophilia	cefaclor	3	0.215	14	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant S224D, cefaclor
metallo-β-lactamase L1variant S224D	Stenotrophomonas maltophilia	cefoxitin	1.4	0.05	28	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant S224D, cefoxitin
metallo-β-lactamase L1variant S224D	Stenotrophomonas maltophilia	cephalothin	32	0.075	430	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant S224D, cephalothin
metallo-β-lactamase L1variant S224D	Stenotrophomonas maltophilia	imipenem	17	0.042	400	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant S224D, imipenem
metallo-β-lactamase L1variant S224D	Stenotrophomonas maltophilia	meropenem	69	0.132	520	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant S224D, meropenem
metallo-β-lactamase L1variant S224D	Stenotrophomonas maltophilia	nitrocefin	2.3	0.011	210	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant S224D, nitrocefin
metallo-β-lactamase L1variant S224K	Stenotrophomonas maltophilia	ampicillin	152	0.025	6.1 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant S224K, ampicillin
metallo-β-lactamase L1variant S224K	Stenotrophomonas maltophilia	benzylpenicillin	124	0.044	2.8 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant S224K, benzylpenicillin
metallo-β-lactamase L1variant S224K	Stenotrophomonas maltophilia	biapenem	43	0.1	430	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant S224K, biapenem
metallo-β-lactamase L1variant S224K	Stenotrophomonas maltophilia	cefaclor	6.5	0.013	500	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant S224K, cefaclor
metallo-β-lactamase L1variant S224K	Stenotrophomonas maltophilia	cefoxitin	0.6	0.002	300	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant S224K, cefoxitin
metallo-β-lactamase L1variant S224K	Stenotrophomonas maltophilia	cephalothin	26	0.0062	4.2 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant S224K, cephalothin
metallo-β-lactamase L1variant S224K	Stenotrophomonas maltophilia	imipenem	14	0.06	230	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant S224K, imipenem
metallo-β-lactamase L1variant S224K	Stenotrophomonas maltophilia	meropenem	212	0.012	1.8 × 10⁴	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant S224K, meropenem
metallo-β-lactamase L1variant S224K	Stenotrophomonas maltophilia	nitrocefin	48	0.014	3.4 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant S224K, nitrocefin
metallo-β-lactamase L1variant Y228A	Stenotrophomonas maltophilia	ampicillin	443	0.71	620	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant Y228A, ampicillin
metallo-β-lactamase L1variant Y228A	Stenotrophomonas maltophilia	benzylpenicillin	609	0.41	1.5 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant Y228A, benzylpenicillin
metallo-β-lactamase L1variant Y228A	Stenotrophomonas maltophilia	biapenem	21	0.175	120	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant Y228A, biapenem
metallo-β-lactamase L1variant Y228A	Stenotrophomonas maltophilia	cefaclor	40	0.55	73	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant Y228A, cefaclor
metallo-β-lactamase L1variant Y228A	Stenotrophomonas maltophilia	cefoxitin	5.8	0.01	580	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant Y228A, cefoxitin
metallo-β-lactamase L1variant Y228A	Stenotrophomonas maltophilia	cephalothin	320	0.29	1.1 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant Y228A, cephalothin
metallo-β-lactamase L1variant Y228A	Stenotrophomonas maltophilia	imipenem	134	0.35	380	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant Y228A, imipenem
metallo-β-lactamase L1variant Y228A	Stenotrophomonas maltophilia	meropenem	26	0.0045	5.8 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant Y228A, meropenem
metallo-β-lactamase L1variant Y228A	Stenotrophomonas maltophilia	nitrocefin	72	0.023	3.1 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant Y228A, nitrocefin
metallo-β-lactamase L1variant Y228F	Stenotrophomonas maltophilia	ampicillin	243	0.271	900	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant Y228F, ampicillin
metallo-β-lactamase L1variant Y228F	Stenotrophomonas maltophilia	benzylpenicillin	630	0.14	4.5 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant Y228F, benzylpenicillin
metallo-β-lactamase L1variant Y228F	Stenotrophomonas maltophilia	biapenem	51	0.15	340	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant Y228F, biapenem
metallo-β-lactamase L1variant Y228F	Stenotrophomonas maltophilia	cefaclor	74	0.24	310	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant Y228F, cefaclor
metallo-β-lactamase L1variant Y228F	Stenotrophomonas maltophilia	cefoxitin	5.5	0.0082	670	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant Y228F, cefoxitin
metallo-β-lactamase L1variant Y228F	Stenotrophomonas maltophilia	cephalothin	190	0.058	3.3 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant Y228F, cephalothin
metallo-β-lactamase L1variant Y228F	Stenotrophomonas maltophilia	imipenem	83	0.107	780	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant Y228F, imipenem
metallo-β-lactamase L1variant Y228F	Stenotrophomonas maltophilia	meropenem	70	0.013	5.4 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant Y228F, meropenem
metallo-β-lactamase L1variant Y228F	Stenotrophomonas maltophilia	nitrocefin	81	0.036	2.3 × 10³	pH 7, 25 °C	Open record: metallo-β-lactamase L1 variant Y228F, nitrocefin