Values CatLog read from this paper
Investigation of the roles of catalytic residues in serotonin N-acetyltransferase
PMID 11884405doi:10.1074/jbc.m200595200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 19 records (29 values) from this paper: serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 10 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT)variant H120A | — | acetyl coenzyme A | — | 1.3 | — | pH 6.8, 30 °C | Open record: serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT) variant H120A, acetyl coenzyme A |
| serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT)variant H120A | — | tryptamine | 5.6 | 7.8 | 0.72 calc. | pH 6.8, 30 °C | Open record: serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT) variant H120A, tryptamine |
| serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT)variant H120A/H122A | — | acetyl coenzyme A | — | 7.6 | — | pH 6.8, 30 °C | Open record: serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT) variant H120A/H122A, acetyl coenzyme A |
| serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT)variant H120A/H122A | — | tryptamine | 0.094 | 4.6 | 0.020 calc. | pH 6.8, 30 °C | Open record: serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT) variant H120A/H122A, tryptamine |
| serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT)variant H120Q | — | acetyl coenzyme A | — | 0.67 | — | pH 6.8, 30 °C | Open record: serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT) variant H120Q, acetyl coenzyme A |
| serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT)variant H120Q | — | tryptamine | 24.5 | 1.2 | 20 calc. | pH 6.8, 30 °C | Open record: serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT) variant H120Q, tryptamine |
| serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT)variant H120Q/H122Q | — | acetyl coenzyme A | — | 1.11 | — | pH 6.8, 30 °C | Open record: serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT) variant H120Q/H122Q, acetyl coenzyme A |
| serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT)variant H120Q/H122Q | — | tryptamine | 3.1 | 10.9 | 0.28 calc. | pH 6.8, 30 °C | Open record: serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT) variant H120Q/H122Q, tryptamine |
| serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT)variant H122A | — | acetyl coenzyme A | — | 0.86 | — | pH 6.8, 30 °C | Open record: serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT) variant H122A, acetyl coenzyme A |
| serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT)variant H122A | — | tryptamine | 8.9 | 2.8 | 3.2 calc. | pH 6.8, 30 °C | Open record: serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT) variant H122A, tryptamine |
| serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT)variant H122E | — | acetyl coenzyme A | — | 1.5 | — | pH 6.8, 30 °C | Open record: serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT) variant H122E, acetyl coenzyme A |
| serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT)variant H122E | — | tryptamine | 2.1 | 3 | 0.70 calc. | pH 6.8, 30 °C | Open record: serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT) variant H122E, tryptamine |
| serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT)variant H122Q | — | acetyl coenzyme A | — | 0.791 | — | pH 6.8, 30 °C | Open record: serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT) variant H122Q, acetyl coenzyme A |
| serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT)variant H122Q | — | tryptamine | 18.1 | 4.78 | 3.79 calc. | pH 6.8, 30 °C | Open record: serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT) variant H122Q, tryptamine |
| serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT)variant Y168F | — | acetyl coenzyme A | — | 0.03 | — | pH 6.8, 30 °C | Open record: serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT) variant Y168F, acetyl coenzyme A |
| serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT)variant Y168F | — | coenzyme A | — | 0.03 | — | pH 6.8, 30 °C | Open record: serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT) variant Y168F, coenzyme A |
| serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT)variant Y168F | — | N-bromoacetyltryptamine | 0.009 | 0.03 | 0.30 calc. | pH 6.8, 30 °C | Open record: serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT) variant Y168F, N-bromoacetyltryptamine |
| serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT)variant Y168F | — | tryptamine | 0.75 | 2.3 | 0.33 calc. | pH 6.8, 30 °C | Open record: serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT) variant Y168F, tryptamine |
| serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT)variant Y168F | — | α-methyltryptamine (racemic) | 0.013 | 4.2 | 0.0031 calc. | pH 6.8, 30 °C | Open record: serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT) variant Y168F, α-methyltryptamine (racemic) |