Values CatLog read from this paper

Substrate specificity engineering of beta-mannosidase and beta-glucosidase from Pyrococcus by exchange of unique active site residues

PMID 11900558doi:10.1021/bi011935aOpen in Browse

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CatLog read 26 records (78 values) from this paper: β-glucosidase CelB, β-mannosidase BglB from Pyrococcus furiosus, Pyrococcus horikoshii.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.2.1.25

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
β-glucosidase CelB	Pyrococcus furiosus	p-nitrophenyl-β-D-galactopyranoside	2827	5	561	90 °C	Open record: β-glucosidase CelB, p-nitrophenyl-β-D-galactopyranoside
β-glucosidase CelB	Pyrococcus furiosus	p-nitrophenyl-β-D-glucopyranoside	1.14 × 10³	0.19	7337	90 °C	Open record: β-glucosidase CelB, p-nitrophenyl-β-D-glucopyranoside
β-glucosidase CelB	Pyrococcus furiosus	p-nitrophenyl-β-D-mannopyranoside	65.9	1.3	49.8	90 °C	Open record: β-glucosidase CelB, p-nitrophenyl-β-D-mannopyranoside
β-glucosidase CelBvariant N206D	Pyrococcus furiosus	p-nitrophenyl-β-D-galactopyranoside	180	15.7	11.5	90 °C	Open record: β-glucosidase CelB variant N206D, p-nitrophenyl-β-D-galactopyranoside
β-glucosidase CelBvariant N206D	Pyrococcus furiosus	p-nitrophenyl-β-D-glucopyranoside	116	4.6	24.9	90 °C	Open record: β-glucosidase CelB variant N206D, p-nitrophenyl-β-D-glucopyranoside
β-glucosidase CelBvariant N206D	Pyrococcus furiosus	p-nitrophenyl-β-D-mannopyranoside	1.97	0.63	3.1	90 °C	Open record: β-glucosidase CelB variant N206D, p-nitrophenyl-β-D-mannopyranoside
β-glucosidase CelBvariant R77Q	Pyrococcus furiosus	p-nitrophenyl-β-D-galactopyranoside	69.2	21.7	3.2	90 °C	Open record: β-glucosidase CelB variant R77Q, p-nitrophenyl-β-D-galactopyranoside
β-glucosidase CelBvariant R77Q	Pyrococcus furiosus	p-nitrophenyl-β-D-glucopyranoside	207	13.8	14.9	90 °C	Open record: β-glucosidase CelB variant R77Q, p-nitrophenyl-β-D-glucopyranoside
β-glucosidase CelBvariant R77Q	Pyrococcus furiosus	p-nitrophenyl-β-D-mannopyranosideI (above 1 mM pNP-Man, Table 2 footnote a)	1.22	0.64	1.9	90 °C	Open record: β-glucosidase CelB variant R77Q, p-nitrophenyl-β-D-mannopyranoside
β-glucosidase CelBvariant R77Q	Pyrococcus furiosus	p-nitrophenyl-β-D-mannopyranosideII (below 1 mM pNP-Man, Table 2 footnote a)	0.85	0.15	5.7	90 °C	Open record: β-glucosidase CelB variant R77Q, p-nitrophenyl-β-D-mannopyranoside
β-glucosidase CelBvariant R77Q/N206D	Pyrococcus furiosus	p-nitrophenyl-β-D-galactopyranoside	29.2	22	1.3	90 °C	Open record: β-glucosidase CelB variant R77Q/N206D, p-nitrophenyl-β-D-galactopyranoside
β-glucosidase CelBvariant R77Q/N206D	Pyrococcus furiosus	p-nitrophenyl-β-D-glucopyranoside	172	13.9	12.3	90 °C	Open record: β-glucosidase CelB variant R77Q/N206D, p-nitrophenyl-β-D-glucopyranoside
β-glucosidase CelBvariant R77Q/N206D	Pyrococcus furiosus	p-nitrophenyl-β-D-mannopyranosideI (above 1 mM pNP-Man, Table 2 footnote a)	1.9	1.5	1.3	90 °C	Open record: β-glucosidase CelB variant R77Q/N206D, p-nitrophenyl-β-D-mannopyranoside
β-glucosidase CelBvariant R77Q/N206D	Pyrococcus furiosus	p-nitrophenyl-β-D-mannopyranosideII (below 1 mM pNP-Man, Table 2 footnote a)	1.02	0.1	10.2	90 °C	Open record: β-glucosidase CelB variant R77Q/N206D, p-nitrophenyl-β-D-mannopyranoside
β-mannosidase BglB	Pyrococcus horikoshii	p-nitrophenyl-β-D-galactopyranoside	13.5	7.3	1.9	90 °C	Open record: β-mannosidase BglB, p-nitrophenyl-β-D-galactopyranoside
β-mannosidase BglB	Pyrococcus horikoshii	p-nitrophenyl-β-D-glucopyranoside	34.4	13.5	2.58	90 °C	Open record: β-mannosidase BglB, p-nitrophenyl-β-D-glucopyranoside
β-mannosidase BglB	Pyrococcus horikoshii	p-nitrophenyl-β-D-mannopyranoside	4.2	0.44	9.8	90 °C	Open record: β-mannosidase BglB, p-nitrophenyl-β-D-mannopyranoside
β-mannosidase BglBvariant D206N	Pyrococcus horikoshii	p-nitrophenyl-β-D-galactopyranoside	571	7.8	75.8	90 °C	Open record: β-mannosidase BglB variant D206N, p-nitrophenyl-β-D-galactopyranoside
β-mannosidase BglBvariant D206N	Pyrococcus horikoshii	p-nitrophenyl-β-D-glucopyranoside	252	0.3	870	90 °C	Open record: β-mannosidase BglB variant D206N, p-nitrophenyl-β-D-glucopyranoside
β-mannosidase BglBvariant D206N	Pyrococcus horikoshii	p-nitrophenyl-β-D-mannopyranoside	45	3.2	14.7	90 °C	Open record: β-mannosidase BglB variant D206N, p-nitrophenyl-β-D-mannopyranoside
β-mannosidase BglBvariant Q77R	Pyrococcus horikoshii	p-nitrophenyl-β-D-galactopyranoside	14.2	16.8	0.85	90 °C	Open record: β-mannosidase BglB variant Q77R, p-nitrophenyl-β-D-galactopyranoside
β-mannosidase BglBvariant Q77R	Pyrococcus horikoshii	p-nitrophenyl-β-D-glucopyranoside	25.5	16.2	1.6	90 °C	Open record: β-mannosidase BglB variant Q77R, p-nitrophenyl-β-D-glucopyranoside
β-mannosidase BglBvariant Q77R	Pyrococcus horikoshii	p-nitrophenyl-β-D-mannopyranoside	1.6	1.1	1.4	90 °C	Open record: β-mannosidase BglB variant Q77R, p-nitrophenyl-β-D-mannopyranoside
β-mannosidase BglBvariant Q77R/D206N	Pyrococcus horikoshii	p-nitrophenyl-β-D-galactopyranoside	66.5	36.1	1.8	90 °C	Open record: β-mannosidase BglB variant Q77R/D206N, p-nitrophenyl-β-D-galactopyranoside
β-mannosidase BglBvariant Q77R/D206N	Pyrococcus horikoshii	p-nitrophenyl-β-D-glucopyranoside	439	30.4	14.4	90 °C	Open record: β-mannosidase BglB variant Q77R/D206N, p-nitrophenyl-β-D-glucopyranoside
β-mannosidase BglBvariant Q77R/D206N	Pyrococcus horikoshii	p-nitrophenyl-β-D-mannopyranoside	6.8	0.68	9.9	90 °C	Open record: β-mannosidase BglB variant Q77R/D206N, p-nitrophenyl-β-D-mannopyranoside