Values CatLog read from this paper
Heterologous expression of an endogenous rat cytochrome b(5)/cytochrome b(5) reductase fusion protein: identification of histidines 62 and 85 as the heme axial ligands
PMID 11913972doi:10.1006/abbi.2002.2783Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 16 records (22 values) from this paper: cytochrome b5/cytochrome b5 reductase fusion protein (cb5/cb5r) from Rattus norvegicus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 6 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| cytochrome b5/cytochrome b5 reductase fusion protein (cb5/cb5r) | Rattus norvegicus | 4S-NADPD | — | 0.001 | — | pH 7, 25 °C | Open record: cytochrome b5/cytochrome b5 reductase fusion protein (cb5/cb5r), 4S-NADPD |
| cytochrome b5/cytochrome b5 reductase fusion protein (cb5/cb5r) | Rattus norvegicus | cytochrome c (horse heart, type VI) | — | 0.0073 | — | pH 7, 25 °C | Open record: cytochrome b5/cytochrome b5 reductase fusion protein (cb5/cb5r), cytochrome c (horse heart, type VI) |
| cytochrome b5/cytochrome b5 reductase fusion protein (cb5/cb5r) | Rattus norvegicus | ferricyanide (K3Fe(CN)6) | — | 0.0282 | — | pH 7, 25 °C | Open record: cytochrome b5/cytochrome b5 reductase fusion protein (cb5/cb5r), ferricyanide (K3Fe(CN)6) |
| cytochrome b5/cytochrome b5 reductase fusion protein (cb5/cb5r) | Rattus norvegicus | NADHNAD(P)H:ferricyanide reductase activity (A340 decrease) | — | 0.0148 | — | pH 7, 25 °C | Open record: cytochrome b5/cytochrome b5 reductase fusion protein (cb5/cb5r), NADH |
| cytochrome b5/cytochrome b5 reductase fusion protein (cb5/cb5r) | Rattus norvegicus | NADHNAD(P)H:cytochrome c reductase activity (A550 increase) | — | 0.0133 | — | pH 7, 25 °C | Open record: cytochrome b5/cytochrome b5 reductase fusion protein (cb5/cb5r), NADH |
| cytochrome b5/cytochrome b5 reductase fusion protein (cb5/cb5r) | Rattus norvegicus | NADPHNAD(P)H:ferricyanide reductase activity (A340 decrease) | 21.7 | 0.0012 | 1.8 × 10⁴ calc. | pH 7, 25 °C | Open record: cytochrome b5/cytochrome b5 reductase fusion protein (cb5/cb5r), NADPH |
| cytochrome b5/cytochrome b5 reductase fusion protein (cb5/cb5r) | Rattus norvegicus | NADPHNAD(P)H:cytochrome c reductase activity (A550 increase) | 8.3 | 0.0011 | 7.5 × 10³ calc. | pH 7, 25 °C | Open record: cytochrome b5/cytochrome b5 reductase fusion protein (cb5/cb5r), NADPH |
| cytochrome b5/cytochrome b5 reductase fusion protein (cb5/cb5r) | Rattus norvegicus | NADPHNADP(H,D):ferricyanide reductase activity | — | 0.001 | — | pH 7, 25 °C | Open record: cytochrome b5/cytochrome b5 reductase fusion protein (cb5/cb5r), NADPH |
| cytochrome b5/cytochrome b5 reductase fusion protein (cb5/cb5r)variant H55A | Rattus norvegicus | ferricyanide (K3Fe(CN)6) | — | 0.0234 | — | pH 7, 25 °C | Open record: cytochrome b5/cytochrome b5 reductase fusion protein (cb5/cb5r) variant H55A, ferricyanide (K3Fe(CN)6) |
| cytochrome b5/cytochrome b5 reductase fusion protein (cb5/cb5r)variant H55A | Rattus norvegicus | NADPH | 23.3 | 0.0014 | 1.7 × 10⁴ calc. | pH 7, 25 °C | Open record: cytochrome b5/cytochrome b5 reductase fusion protein (cb5/cb5r) variant H55A, NADPH |
| cytochrome b5/cytochrome b5 reductase fusion protein (cb5/cb5r)variant H55M | Rattus norvegicus | ferricyanide (K3Fe(CN)6) | — | 0.0273 | — | pH 7, 25 °C | Open record: cytochrome b5/cytochrome b5 reductase fusion protein (cb5/cb5r) variant H55M, ferricyanide (K3Fe(CN)6) |
| cytochrome b5/cytochrome b5 reductase fusion protein (cb5/cb5r)variant H55M | Rattus norvegicus | NADPH | 20.3 | 0.0015 | 1.4 × 10⁴ calc. | pH 7, 25 °C | Open record: cytochrome b5/cytochrome b5 reductase fusion protein (cb5/cb5r) variant H55M, NADPH |
| cytochrome b5/cytochrome b5 reductase fusion protein (cb5/cb5r)variant H78A | Rattus norvegicus | ferricyanide (K3Fe(CN)6) | — | 0.0233 | — | pH 7, 25 °C | Open record: cytochrome b5/cytochrome b5 reductase fusion protein (cb5/cb5r) variant H78A, ferricyanide (K3Fe(CN)6) |
| cytochrome b5/cytochrome b5 reductase fusion protein (cb5/cb5r)variant H78A | Rattus norvegicus | NADPH | 25 | 0.0017 | 1.5 × 10⁴ calc. | pH 7, 25 °C | Open record: cytochrome b5/cytochrome b5 reductase fusion protein (cb5/cb5r) variant H78A, NADPH |
| cytochrome b5/cytochrome b5 reductase fusion protein (cb5/cb5r)variant H78M | Rattus norvegicus | ferricyanide (K3Fe(CN)6) | — | 0.0264 | — | pH 7, 25 °C | Open record: cytochrome b5/cytochrome b5 reductase fusion protein (cb5/cb5r) variant H78M, ferricyanide (K3Fe(CN)6) |
| cytochrome b5/cytochrome b5 reductase fusion protein (cb5/cb5r)variant H78M | Rattus norvegicus | NADPH | 20 | 0.0015 | 1.3 × 10⁴ calc. | pH 7, 25 °C | Open record: cytochrome b5/cytochrome b5 reductase fusion protein (cb5/cb5r) variant H78M, NADPH |