Values CatLog read from this paper

Role of hydrogen bonds in the reaction mechanism of chalcone isomerase

PMID 11955065doi:10.1021/bi0255266Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 25 records (70 values) from this paper: chalcone isomerase from Medicago sativa.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 5.5.1.6

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
chalcone isomerasevariant N113A	Medicago sativa	2′,4′-dihydroxychalcone	4.73	0.0174	272	pH 7.5, 25 °C	Open record: chalcone isomerase variant N113A, 2′,4′-dihydroxychalcone
chalcone isomerasevariant N113A	Medicago sativa	4,2′,4′,6′-tetrahydroxychalcone	13.05	0.449	29.1	pH 7.5, 25 °C	Open record: chalcone isomerase variant N113A, 4,2′,4′,6′-tetrahydroxychalcone
chalcone isomerasevariant N113A	Medicago sativa	4,2′,4′-trihydroxychalcone	5.07	0.0243	209	pH 7.5, 25 °C	Open record: chalcone isomerase variant N113A, 4,2′,4′-trihydroxychalcone
chalcone isomerasevariant N113A	Medicago sativa	4,2′,4′-trihydroxychalcone	12.78	—	392		Open record: chalcone isomerase variant N113A, 4,2′,4′-trihydroxychalcone
chalcone isomerasevariant N113A	Medicago sativa	4,2′-dihydroxychalcone	5.72	0.0525	109	pH 7.5, 25 °C	Open record: chalcone isomerase variant N113A, 4,2′-dihydroxychalcone
chalcone isomerasevariant T190A	Medicago sativa	2′,4′-dihydroxychalcone	2.93	0.0192	153	pH 7.5, 25 °C	Open record: chalcone isomerase variant T190A, 2′,4′-dihydroxychalcone
chalcone isomerasevariant T190A	Medicago sativa	4,2′,4′,6′-tetrahydroxychalcone	8.43	0.263	32.1	pH 7.5, 25 °C	Open record: chalcone isomerase variant T190A, 4,2′,4′,6′-tetrahydroxychalcone
chalcone isomerasevariant T190A	Medicago sativa	4,2′,4′-trihydroxychalcone	6.32	0.0198	319	pH 7.5, 25 °C	Open record: chalcone isomerase variant T190A, 4,2′,4′-trihydroxychalcone
chalcone isomerasevariant T190A	Medicago sativa	4,2′,4′-trihydroxychalcone	8.73	—	478		Open record: chalcone isomerase variant T190A, 4,2′,4′-trihydroxychalcone
chalcone isomerasevariant T190A	Medicago sativa	4,2′-dihydroxychalcone	3.75	0.0559	67.1	pH 7.5, 25 °C	Open record: chalcone isomerase variant T190A, 4,2′-dihydroxychalcone
chalcone isomerasevariant T48A	Medicago sativa	2′,4′-dihydroxychalcone	0.0178	0.015	1.19	pH 7.5, 25 °C	Open record: chalcone isomerase variant T48A, 2′,4′-dihydroxychalcone
chalcone isomerasevariant T48A	Medicago sativa	4,2′,4′,6′-tetrahydroxychalcone	1.477	0.182	8.11	pH 7.5, 25 °C	Open record: chalcone isomerase variant T48A, 4,2′,4′,6′-tetrahydroxychalcone
chalcone isomerasevariant T48A	Medicago sativa	4,2′,4′-trihydroxychalcone	0.0242	0.0189	1.28	pH 7.5, 25 °C	Open record: chalcone isomerase variant T48A, 4,2′,4′-trihydroxychalcone
chalcone isomerasevariant T48A	Medicago sativa	4,2′,4′-trihydroxychalcone	0.0498	—	2.24		Open record: chalcone isomerase variant T48A, 4,2′,4′-trihydroxychalcone
chalcone isomerasevariant T48A	Medicago sativa	4,2′-dihydroxychalcone	0.0047	0.0464	0.101	pH 7.5, 25 °C	Open record: chalcone isomerase variant T48A, 4,2′-dihydroxychalcone
chalcone isomerasevariant T48S	Medicago sativa	2′,4′-dihydroxychalcone	8.65	0.041	197	pH 7.5, 25 °C	Open record: chalcone isomerase variant T48S, 2′,4′-dihydroxychalcone
chalcone isomerasevariant T48S	Medicago sativa	4,2′,4′,6′-tetrahydroxychalcone	215	0.516	420	pH 7.5, 25 °C	Open record: chalcone isomerase variant T48S, 4,2′,4′,6′-tetrahydroxychalcone
chalcone isomerasevariant T48S	Medicago sativa	4,2′,4′-trihydroxychalcone	13.83	0.0213	650	pH 7.5, 25 °C	Open record: chalcone isomerase variant T48S, 4,2′,4′-trihydroxychalcone
chalcone isomerasevariant T48S	Medicago sativa	4,2′,4′-trihydroxychalcone	8.33	—	285		Open record: chalcone isomerase variant T48S, 4,2′,4′-trihydroxychalcone
chalcone isomerasevariant T48S	Medicago sativa	4,2′-dihydroxychalcone	0.902	0.056	16.1	pH 7.5, 25 °C	Open record: chalcone isomerase variant T48S, 4,2′-dihydroxychalcone
chalcone isomerasevariant Y106F	Medicago sativa	2′,4′-dihydroxychalcone	0.568	0.0346	16.4	pH 7.5, 25 °C	Open record: chalcone isomerase variant Y106F, 2′,4′-dihydroxychalcone
chalcone isomerasevariant Y106F	Medicago sativa	4,2′,4′,6′-tetrahydroxychalcone	6.6	0.168	39.3	pH 7.5, 25 °C	Open record: chalcone isomerase variant Y106F, 4,2′,4′,6′-tetrahydroxychalcone
chalcone isomerasevariant Y106F	Medicago sativa	4,2′,4′-trihydroxychalcone	1.253	0.0124	101	pH 7.5, 25 °C	Open record: chalcone isomerase variant Y106F, 4,2′,4′-trihydroxychalcone
chalcone isomerasevariant Y106F	Medicago sativa	4,2′,4′-trihydroxychalcone	3.65	—	575		Open record: chalcone isomerase variant Y106F, 4,2′,4′-trihydroxychalcone
chalcone isomerasevariant Y106F	Medicago sativa	4,2′-dihydroxychalcone	0.892	0.0469	19	pH 7.5, 25 °C	Open record: chalcone isomerase variant Y106F, 4,2′-dihydroxychalcone