CatLogenzyme kinetics catalog

Values CatLog read from this paper

Optimizing an artificial metabolic pathway: engineering the cofactor specificity of Corynebacterium 2,5-diketo-D-gluconic acid reductase for use in vitamin C biosynthesis

PMID 12009883doi:10.1021/bi015987bOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 46 records (61 values) from this paper: 2,5-diketo-D-gluconic acid reductase A from Corynebacterium sp..

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 15 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 1.1.1.274

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
2,5-diketo-D-gluconic acid reductase ACorynebacterium sp.2,5-diketo-D-gluconic acidNADH oxidation followed at 375; cosubstrate NADH; Table 3——0.017pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase ACorynebacterium sp.2,5-diketo-D-gluconic acidNADH oxidation followed at 375; cosubstrate NADH; Table 62.81300.022 calc.pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase ACorynebacterium sp.2,5-diketo-D-gluconic acidNADPH oxidation followed at 340; cosubstrate NADPH12220.53 calc.pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase ACorynebacterium sp.NADH—1.4—pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase ACorynebacterium sp.NADPH—0.022—pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase ACorynebacterium sp.NADPH—0.013—pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase Avariant F22Y/A272GCorynebacterium sp.2,5-diketo-D-gluconic acidNADH oxidation followed at 375; cosubstrate NADH; Table 3——0.028pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase Avariant F22Y/A272GCorynebacterium sp.2,5-diketo-D-gluconic acidNADH oxidation followed at 375; cosubstrate NADH; Table 61.23300.041 calc.pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase Avariant F22Y/A272GCorynebacterium sp.2,5-diketo-D-gluconic acidNADPH oxidation followed at 340; cosubstrate NADPH; Table 818.37.22.5 calc.pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase Avariant F22Y/A272GCorynebacterium sp.NADH—2.4—pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase Avariant F22Y/A272GCorynebacterium sp.NADPH—0.039—pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase Avariant F22Y/A272GCorynebacterium sp.NADPHapparent Km—0.037—pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase Avariant F22Y/K232G/R235G/R238E/A272GCorynebacterium sp.2,5-diketo-D-gluconic acidTable 3——0.45pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase Avariant F22Y/K232G/R235G/R238E/A272GCorynebacterium sp.2,5-diketo-D-gluconic acidTable 610130.77 calc.pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase Avariant F22Y/K232G/R235G/R238E/A272GCorynebacterium sp.NADH—3.4—pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase Avariant F22Y/K232G/R235G/R238H/A272GCorynebacterium sp.2,5-diketo-D-gluconic acidNADH oxidation followed at 375; cosubstrate NADH; Table 3——0.33pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase Avariant F22Y/K232G/R235G/R238H/A272GCorynebacterium sp.2,5-diketo-D-gluconic acidNADH oxidation followed at 375; cosubstrate NADH; Table 618430.43 calc.pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase Avariant F22Y/K232G/R235G/R238H/A272GCorynebacterium sp.2,5-diketo-D-gluconic acidNADPH oxidation followed at 340; cosubstrate NADPH; Table 818320.57 calc.pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase Avariant F22Y/K232G/R235G/R238H/A272GCorynebacterium sp.NADH—2.7—pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase Avariant F22Y/K232G/R235G/R238H/A272GCorynebacterium sp.NADPH—0.15—pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase Avariant F22Y/K232G/R235T/R238H/A272GCorynebacterium sp.2,5-diketo-D-gluconic acidNADH oxidation followed at 375; cosubstrate NADH; Table 3——0.42pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase Avariant F22Y/K232G/R235T/R238H/A272GCorynebacterium sp.2,5-diketo-D-gluconic acidNADH oxidation followed at 375; cosubstrate NADH; Table 6681500.46 calc.pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase Avariant F22Y/K232G/R235T/R238H/A272GCorynebacterium sp.2,5-diketo-D-gluconic acidNADPH oxidation followed at 340; cosubstrate NADPH; Table 878.70.80 calc.pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase Avariant F22Y/K232G/R235T/R238H/A272GCorynebacterium sp.NADH—5.2—pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase Avariant F22Y/K232G/R235T/R238H/A272GCorynebacterium sp.NADPH—0.071—pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase Avariant F22Y/K232G/R238H/A272GCorynebacterium sp.2,5-diketo-D-gluconic acidNADH oxidation followed at 375; cosubstrate NADH; Table 3——0.4pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase Avariant F22Y/K232G/R238H/A272GCorynebacterium sp.2,5-diketo-D-gluconic acidNADH oxidation followed at 375; cosubstrate NADH; Table 616.5320.52 calc.pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase Avariant F22Y/K232G/R238H/A272GCorynebacterium sp.2,5-diketo-D-gluconic acidNADPH oxidation followed at 340; cosubstrate NADPH; Table 87.39.10.81 calc.pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase Avariant F22Y/K232G/R238H/A272GCorynebacterium sp.NADH—1.2—pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase Avariant F22Y/K232G/R238H/A272GCorynebacterium sp.NADPH—0.058—pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase Avariant F22Y/S233T/R235S/R238H/A272GCorynebacterium sp.2,5-diketo-D-gluconic acidNADH oxidation followed at 375; cosubstrate NADH; Table 3——0.25pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase Avariant F22Y/S233T/R235S/R238H/A272GCorynebacterium sp.2,5-diketo-D-gluconic acidNADH oxidation followed at 375; cosubstrate NADH; Table 68.5270.31 calc.pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase Avariant F22Y/S233T/R235S/R238H/A272GCorynebacterium sp.2,5-diketo-D-gluconic acidNADPH oxidation followed at 340; cosubstrate NADPH; Table 82.20.395.6 calc.pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase Avariant F22Y/S233T/R235S/R238H/A272GCorynebacterium sp.NADH—3.9—pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase Avariant F22Y/S233T/R235S/R238H/A272GCorynebacterium sp.NADPH—0.055—pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase Avariant K232G/R235G/R238ECorynebacterium sp.2,5-diketo-D-gluconic acid——0.2pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase Avariant K232G/R235G/R238HCorynebacterium sp.2,5-diketo-D-gluconic acid——0.133pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase Avariant K232G/R235T/R238HCorynebacterium sp.2,5-diketo-D-gluconic acid——0.14pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase Avariant K232G/R238ECorynebacterium sp.2,5-diketo-D-gluconic acid——0.122pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase Avariant K232G/R238HCorynebacterium sp.2,5-diketo-D-gluconic acidNADH oxidation followed at 375; cosubstrate NADH; Table 3——0.152pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase Avariant K232G/R238HCorynebacterium sp.2,5-diketo-D-gluconic acidNADH oxidation followed at 375; cosubstrate NADH; Table 6201100.18 calc.pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase Avariant K232G/R238HCorynebacterium sp.2,5-diketo-D-gluconic acidNADPH oxidation followed at 340; cosubstrate NADPH; Table 83.3130.26 calc.pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase Avariant K232G/R238HCorynebacterium sp.NADH—0.66—pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase Avariant K232G/R238HCorynebacterium sp.NADPH—0.13—pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase Avariant K232S/R238HCorynebacterium sp.2,5-diketo-D-gluconic acid——0.055pH 7, 25 °COpen
2,5-diketo-D-gluconic acid reductase Avariant S233T/R235S/R238HCorynebacterium sp.2,5-diketo-D-gluconic acid——0.143pH 7, 25 °COpen