Values CatLog read from this paper
Engineering the substrate specificity of D-amino-acid oxidase
PMID 12021281doi:10.1074/jbc.m203946200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 19 records (53 values) from this paper: D-amino-acid oxidase (RgDAAO) from Rhodotorula gracilis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| D-amino-acid oxidase (RgDAAO) | Rhodotorula gracilis | D-alanine | 83 | 0.8 | 102 | pH 8.5, 25 °C | Open record: D-amino-acid oxidase (RgDAAO), D-alanine |
| D-amino-acid oxidase (RgDAAO) | Rhodotorula gracilis | D-asparagine | 22 | 14.4 | 1.5 | pH 8.5, 25 °C | Open record: D-amino-acid oxidase (RgDAAO), D-asparagine |
| D-amino-acid oxidase (RgDAAO) | Rhodotorula gracilis | D-aspartate | 0.48 | 18 | 0.027 | pH 8.5, 25 °C | Open record: D-amino-acid oxidase (RgDAAO), D-aspartate |
| D-amino-acid oxidase (RgDAAO) | Rhodotorula gracilis | D-glutamate | 1 | 77.3 | 0.013 | pH 8.5, 25 °C | Open record: D-amino-acid oxidase (RgDAAO), D-glutamate |
| D-amino-acid oxidase (RgDAAO) | Rhodotorula gracilis | D-glutamine | 18.92 | 4.6 | 4.12 | pH 8.5, 25 °C | Open record: D-amino-acid oxidase (RgDAAO), D-glutamine |
| D-amino-acid oxidase (RgDAAO) | Rhodotorula gracilis | D-methionine | 77 | 0.2 | 425 | pH 8.5, 25 °C | Open record: D-amino-acid oxidase (RgDAAO), D-methionine |
| D-amino-acid oxidase (RgDAAO) | Rhodotorula gracilis | D-proline | 77.3 | 21.5 | 3.6 | pH 8.5, 25 °C | Open record: D-amino-acid oxidase (RgDAAO), D-proline |
| D-amino-acid oxidase (RgDAAO)variant M213R | Rhodotorula gracilis | D-alanine | 10.5 | 17.8 | 0.59 | pH 8.5, 25 °C | Open record: D-amino-acid oxidase (RgDAAO) variant M213R, D-alanine |
| D-amino-acid oxidase (RgDAAO)variant M213R | Rhodotorula gracilis | D-alanine | — | 10.1 | — | pH 8.5 | Open record: D-amino-acid oxidase (RgDAAO) variant M213R, D-alanine |
| D-amino-acid oxidase (RgDAAO)variant M213R | Rhodotorula gracilis | D-asparagine | 1.77 | 11 | 0.16 | pH 8.5, 25 °C | Open record: D-amino-acid oxidase (RgDAAO) variant M213R, D-asparagine |
| D-amino-acid oxidase (RgDAAO)variant M213R | Rhodotorula gracilis | D-aspartate | 3.92 | 2 | 1.97 | pH 8.5, 25 °C | Open record: D-amino-acid oxidase (RgDAAO) variant M213R, D-aspartate |
| D-amino-acid oxidase (RgDAAO)variant M213R | Rhodotorula gracilis | D-aspartate | — | 1.5 | — | pH 8.5 | Open record: D-amino-acid oxidase (RgDAAO) variant M213R, D-aspartate |
| D-amino-acid oxidase (RgDAAO)variant M213R | Rhodotorula gracilis | D-glutamate | 16.25 | 33 | 0.492 | pH 8.5, 25 °C | Open record: D-amino-acid oxidase (RgDAAO) variant M213R, D-glutamate |
| D-amino-acid oxidase (RgDAAO)variant M213R | Rhodotorula gracilis | D-glutamine | 4.8 | 3.8 | 1.28 | pH 8.5, 25 °C | Open record: D-amino-acid oxidase (RgDAAO) variant M213R, D-glutamine |
| D-amino-acid oxidase (RgDAAO)variant M213R | Rhodotorula gracilis | D-methionine | 11.3 | 1.5 | 7.58 | pH 8.5, 25 °C | Open record: D-amino-acid oxidase (RgDAAO) variant M213R, D-methionine |
| D-amino-acid oxidase (RgDAAO)variant M213R | Rhodotorula gracilis | D-proline | 26.92 | 1.28 × 10³ | 0.022 | pH 8.5, 25 °C | Open record: D-amino-acid oxidase (RgDAAO) variant M213R, D-proline |
| D-amino-acid oxidase (RgDAAO)variant M213R | Rhodotorula gracilis | N-methyl-D-aspartate | 1.32 | 18 | 0.73 | pH 8.5, 25 °C | Open record: D-amino-acid oxidase (RgDAAO) variant M213R, N-methyl-D-aspartate |
| D-amino-acid oxidase (RgDAAO)variant M213R/Y238R | Rhodotorula gracilis | D-alanine | 4.63 | 45.8 | 0.102 | pH 8.5, 25 °C | Open record: D-amino-acid oxidase (RgDAAO) variant M213R/Y238R, D-alanine |
| D-amino-acid oxidase (RgDAAO)variant M213R/Y238R | Rhodotorula gracilis | D-aspartate | 27.75 | 28 | 0.975 | pH 8.5, 25 °C | Open record: D-amino-acid oxidase (RgDAAO) variant M213R/Y238R, D-aspartate |