Values CatLog read from this paper

Purification, characterization and catalytic properties of human sterol 8-isomerase

PMID 12133002doi:10.1042/bj20020551Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 4 records (5 values) from this paper: sterol 8-isomerase (hSI; emopamil-binding protein) from Homo sapiens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.

EC 5.3.3.5

Records read from this paper
EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsOpen record
sterol 8-isomerase (hSI; emopamil-binding protein)Homo sapienscholest-8-en-3β-ol—0.025—pH 7.5, 37 °COpen record: sterol 8-isomerase (hSI; emopamil-binding protein), cholest-8-en-3β-ol
sterol 8-isomerase (hSI; emopamil-binding protein)Homo sapienscholesta-7,24-dien-3β-ol—0.0661—pH 7.5, 37 °COpen record: sterol 8-isomerase (hSI; emopamil-binding protein), cholesta-7,24-dien-3β-ol
sterol 8-isomerase (hSI; emopamil-binding protein)Homo sapienszymosterolstandard assay with Tween-80 (1%, w/v); 37 °C; 3 h0.4230.058.5 calc.pH 7.5, 37 °COpen record: sterol 8-isomerase (hSI; emopamil-binding protein), zymosterol
sterol 8-isomerase (hSI; emopamil-binding protein)Homo sapienszymosterolcrude homogenate of E. coli BL21(DE3) cells harbouring CHO2—0.0498—pH 7.5, 37 °COpen record: sterol 8-isomerase (hSI; emopamil-binding protein), zymosterol