Values CatLog read from this paper
A case for reverse protonation: identification of Glu160 as an acid/base catalyst in Thermoanaerobacterium saccharolyticum beta-xylosidase and detailed kinetic analysis of a site-directed mutant
PMID 12146939doi:10.1021/bi020078nOpen in Browse
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CatLog read 10 records (30 values) from this paper: beta-xylosidase (XynB) from Thermoanaerobacterium saccharolyticum.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| beta-xylosidase (XynB) | Thermoanaerobacterium saccharolyticum | 2,5-dinitrophenyl beta-D-xylopyranoside | 7.3 | 0.011 | 670 | pH 5.5, 37 °C | Open record: beta-xylosidase (XynB), 2,5-dinitrophenyl beta-D-xylopyranoside |
| beta-xylosidase (XynB) | Thermoanaerobacterium saccharolyticum | 2-nitrophenyl beta-D-xylopyranoside | 9.9 | 0.046 | 220 | pH 5.5, 37 °C | Open record: beta-xylosidase (XynB), 2-nitrophenyl beta-D-xylopyranoside |
| beta-xylosidase (XynB) | Thermoanaerobacterium saccharolyticum | 3,4-dinitrophenyl beta-D-xylopyranoside | 8.9 | 0.01 | 900 | pH 5.5, 37 °C | Open record: beta-xylosidase (XynB), 3,4-dinitrophenyl beta-D-xylopyranoside |
| beta-xylosidase (XynB) | Thermoanaerobacterium saccharolyticum | 3-nitrophenyl beta-D-xylopyranoside | 11 | 0.15 | 73 | pH 5.5, 37 °C | Open record: beta-xylosidase (XynB), 3-nitrophenyl beta-D-xylopyranoside |
| beta-xylosidase (XynB) | Thermoanaerobacterium saccharolyticum | 4-nitrophenyl beta-D-xylopyranoside | 9.7 | 0.036 | 270 | pH 5.5, 37 °C | Open record: beta-xylosidase (XynB), 4-nitrophenyl beta-D-xylopyranoside |
| beta-xylosidase (XynB)variant E160A | Thermoanaerobacterium saccharolyticum | 2,5-dinitrophenyl beta-D-xylopyranoside | 0.84 | 0.01 | 84 | pH 6.5, 37 °C | Open record: beta-xylosidase (XynB) variant E160A, 2,5-dinitrophenyl beta-D-xylopyranoside |
| beta-xylosidase (XynB)variant E160A | Thermoanaerobacterium saccharolyticum | 2-nitrophenyl beta-D-xylopyranoside | 0.09 | 0.08 | 1.1 | pH 6.5, 37 °C | Open record: beta-xylosidase (XynB) variant E160A, 2-nitrophenyl beta-D-xylopyranoside |
| beta-xylosidase (XynB)variant E160A | Thermoanaerobacterium saccharolyticum | 3,4-dinitrophenyl beta-D-xylopyranoside | 0.78 | 0.014 | 56 | pH 6.5, 37 °C | Open record: beta-xylosidase (XynB) variant E160A, 3,4-dinitrophenyl beta-D-xylopyranoside |
| beta-xylosidase (XynB)variant E160A | Thermoanaerobacterium saccharolyticum | 3-nitrophenyl beta-D-xylopyranoside | 0.0052 | 4.9 | 0.0011 | pH 6.5, 37 °C | Open record: beta-xylosidase (XynB) variant E160A, 3-nitrophenyl beta-D-xylopyranoside |
| beta-xylosidase (XynB)variant E160A | Thermoanaerobacterium saccharolyticum | 4-nitrophenyl beta-D-xylopyranoside | 0.04 | 0.14 | 0.28 | pH 6.5, 37 °C | Open record: beta-xylosidase (XynB) variant E160A, 4-nitrophenyl beta-D-xylopyranoside |