CatLogenzyme kinetics catalog

Values CatLog read from this paper

Mutational, kinetic, and NMR studies of the mechanism of E. coli GDP-mannose mannosyl hydrolase, an unusual Nudix enzyme

PMID 12196023doi:10.1021/bi020362eOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 22 records (43 values) from this paper: GDP-mannose mannosyl hydrolase (GDPMH) from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 21 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
GDP-mannose mannosyl hydrolase (GDPMH)Escherichia coliGDP-alpha-D-mannose0.710.322.2 calc.pH 9.3, 37 °COpen
GDP-mannose mannosyl hydrolase (GDPMH)variant A69EEscherichia coliGDP-alpha-D-mannose0.241.190.20 calc.pH 9.3, 37 °COpen
GDP-mannose mannosyl hydrolase (GDPMH)variant A69EEscherichia coliGDP-alpha-D-mannose0.10.170.59 calc.pH 7.5, 22 °COpen
GDP-mannose mannosyl hydrolase (GDPMH)variant A69EEscherichia coliGDP-alpha-D-mannose0.080.250.32 calc.pH 7.5, 22 °COpen
GDP-mannose mannosyl hydrolase (GDPMH)variant E60QEscherichia coliGDP-alpha-D-mannose0.462.40.19 calc.pH 9.3, 37 °COpen
GDP-mannose mannosyl hydrolase (GDPMH)variant E60QEscherichia coliGDP-alpha-D-mannose0.1130.80.14 calc.pH 7.5, 22 °COpen
GDP-mannose mannosyl hydrolase (GDPMH)variant E60QEscherichia coliGDP-alpha-D-mannose0.0540.270.20 calc.pH 7.5, 22 °COpen
GDP-mannose mannosyl hydrolase (GDPMH)variant E64QEscherichia coliGDP-alpha-D-mannose0.640.282.3 calc.pH 9.3, 37 °COpen
GDP-mannose mannosyl hydrolase (GDPMH)variant E64QEscherichia coliGDP-alpha-D-mannose0.310.171.8 calc.pH 7.5, 22 °COpen
GDP-mannose mannosyl hydrolase (GDPMH)variant E64QEscherichia coliGDP-alpha-D-mannose0.210.270.78 calc.pH 7.5, 22 °COpen
GDP-mannose mannosyl hydrolase (GDPMH)variant E70QEscherichia coliGDP-alpha-D-mannose0.00493.20.0015 calc.pH 9.3, 37 °COpen
GDP-mannose mannosyl hydrolase (GDPMH)variant E70QEscherichia coliGDP-alpha-D-mannose0.0025——pH 7.5, 22 °COpen
GDP-mannose mannosyl hydrolase (GDPMH)variant H102QEscherichia coliGDP-alpha-D-mannose0.094.40.020 calc.pH 9.3, 37 °COpen
GDP-mannose mannosyl hydrolase (GDPMH)variant H124QEscherichia coliGDP-alpha-D-mannose3.1 × 10⁻⁴1.52.1 × 10⁻⁴ calc.pH 9.3, 37 °COpen
GDP-mannose mannosyl hydrolase (GDPMH)variant H142QEscherichia coliGDP-alpha-D-mannose0.520.90.58 calc.pH 9.3, 37 °COpen
GDP-mannose mannosyl hydrolase (GDPMH)variant H142QEscherichia coliGDP-alpha-D-mannose0.070.032.3 calc.pH 7.5, 22 °COpen
GDP-mannose mannosyl hydrolase (GDPMH)variant H142QEscherichia coliGDP-alpha-D-mannose0.0980.190.52 calc.pH 7.5, 22 °COpen
GDP-mannose mannosyl hydrolase (GDPMH)variant H88QEscherichia coliGDP-alpha-D-mannose0.00361.40.0026 calc.pH 9.3, 37 °COpen
GDP-mannose mannosyl hydrolase (GDPMH)variant R52KEscherichia coliGDP-alpha-D-mannose0.06140.0043 calc.pH 9.3, 37 °COpen
GDP-mannose mannosyl hydrolase (GDPMH)variant R52QEscherichia coliGDP-alpha-D-mannose0.0023131.8 × 10⁻⁴ calc.pH 9.3, 37 °COpen
GDP-mannose mannosyl hydrolase (GDPMH)variant R65KEscherichia coliGDP-alpha-D-mannose0.061.10.055 calc.pH 9.3, 37 °COpen
GDP-mannose mannosyl hydrolase (GDPMH)variant R65QEscherichia coliGDP-alpha-D-mannose0.03260.0012 calc.pH 9.3, 37 °COpen