Values CatLog read from this paper
Detailed kinetic analysis and identification of the nucleophile in alpha-L-arabinofuranosidase from Geobacillus stearothermophilus T-6, a family 51 glycoside hydrolase
PMID 12221104doi:10.1074/jbc.m208285200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 17 records (38 values) from this paper: α-L-arabinofuranosidase (AbfA T-6) from Geobacillus stearothermophilus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| α-L-arabinofuranosidase (AbfA T-6) | Geobacillus stearothermophilus | 2,4,6-trichlorophenyl α-L-arabinofuranoside | 65 | 1 | 65 | pH 6, 40 °C | Open record: α-L-arabinofuranosidase (AbfA T-6), 2,4,6-trichlorophenyl α-L-arabinofuranoside |
| α-L-arabinofuranosidase (AbfA T-6) | Geobacillus stearothermophilus | 2,5-dinitrophenyl α-L-arabinofuranoside | 190 | 0.35 | 540 | pH 6, 40 °C | Open record: α-L-arabinofuranosidase (AbfA T-6), 2,5-dinitrophenyl α-L-arabinofuranoside |
| α-L-arabinofuranosidase (AbfA T-6) | Geobacillus stearothermophilus | 3,4-dinitrophenyl α-L-arabinofuranoside | 340 | 0.53 | 640 | pH 6, 40 °C | Open record: α-L-arabinofuranosidase (AbfA T-6), 3,4-dinitrophenyl α-L-arabinofuranoside |
| α-L-arabinofuranosidase (AbfA T-6) | Geobacillus stearothermophilus | 3-nitrophenyl α-L-arabinofuranoside | 83 | 1.7 | 49 | pH 6, 40 °C | Open record: α-L-arabinofuranosidase (AbfA T-6), 3-nitrophenyl α-L-arabinofuranoside |
| α-L-arabinofuranosidase (AbfA T-6) | Geobacillus stearothermophilus | 4-nitrophenyl α-L-arabinofuranoside | 87 | 0.65 | 130 | pH 6, 40 °C | Open record: α-L-arabinofuranosidase (AbfA T-6), 4-nitrophenyl α-L-arabinofuranoside |
| α-L-arabinofuranosidase (AbfA T-6)variant E175A | Geobacillus stearothermophilus | 2,4,6-trichlorophenyl α-L-arabinofuranoside | 0.24 | 0.04 | 6 | pH 6, 40 °C | Open record: α-L-arabinofuranosidase (AbfA T-6) variant E175A, 2,4,6-trichlorophenyl α-L-arabinofuranoside |
| α-L-arabinofuranosidase (AbfA T-6)variant E175A | Geobacillus stearothermophilus | 2,5-dinitrophenyl α-L-arabinofuranoside | 0.72 | 0.003 | 240 | pH 6, 40 °C | Open record: α-L-arabinofuranosidase (AbfA T-6) variant E175A, 2,5-dinitrophenyl α-L-arabinofuranoside |
| α-L-arabinofuranosidase (AbfA T-6)variant E175A | Geobacillus stearothermophilus | 3,4-dinitrophenyl α-L-arabinofuranoside | 0.66 | 0.01 | 66 | pH 6, 40 °C | Open record: α-L-arabinofuranosidase (AbfA T-6) variant E175A, 3,4-dinitrophenyl α-L-arabinofuranoside |
| α-L-arabinofuranosidase (AbfA T-6)variant E175A | Geobacillus stearothermophilus | 3-nitrophenyl α-L-arabinofuranoside | 0.0041 | 0.6 | 0.007 | pH 6, 40 °C | Open record: α-L-arabinofuranosidase (AbfA T-6) variant E175A, 3-nitrophenyl α-L-arabinofuranoside |
| α-L-arabinofuranosidase (AbfA T-6)variant E175A | Geobacillus stearothermophilus | 4-nitrophenyl α-L-arabinofuranoside | 0.08 | 0.14 | 0.57 | pH 6, 40 °C | Open record: α-L-arabinofuranosidase (AbfA T-6) variant E175A, 4-nitrophenyl α-L-arabinofuranoside |
| α-L-arabinofuranosidase (AbfA T-6)variant E294A | Geobacillus stearothermophilus | 2,4,6-trichlorophenyl α-L-arabinofuranoside | 9 × 10⁻⁵ | — | — | pH 6, 40 °C | Open record: α-L-arabinofuranosidase (AbfA T-6) variant E294A, 2,4,6-trichlorophenyl α-L-arabinofuranoside |
| α-L-arabinofuranosidase (AbfA T-6)variant E294A | Geobacillus stearothermophilus | 2,4,6-trichlorophenyl α-L-arabinofuranosidewith 1 M NaN3 (azide rescue) | 0.004 | — | — | pH 6, 40 °C | Open record: α-L-arabinofuranosidase (AbfA T-6) variant E294A, 2,4,6-trichlorophenyl α-L-arabinofuranoside |
| α-L-arabinofuranosidase (AbfA T-6)variant E294A | Geobacillus stearothermophilus | 2,5-dinitrophenyl α-L-arabinofuranosideTable II | 3 × 10⁻⁴ | — | — | pH 6, 40 °C | Open record: α-L-arabinofuranosidase (AbfA T-6) variant E294A, 2,5-dinitrophenyl α-L-arabinofuranoside |
| α-L-arabinofuranosidase (AbfA T-6)variant E294A | Geobacillus stearothermophilus | 2,5-dinitrophenyl α-L-arabinofuranosidein the text | — | 8 | 2.5 × 10⁻⁵ | pH 6, 40 °C | Open record: α-L-arabinofuranosidase (AbfA T-6) variant E294A, 2,5-dinitrophenyl α-L-arabinofuranoside |
| α-L-arabinofuranosidase (AbfA T-6)variant E294A | Geobacillus stearothermophilus | 3,4-dinitrophenyl α-L-arabinofuranoside | 1 × 10⁻⁵ | — | — | pH 6, 40 °C | Open record: α-L-arabinofuranosidase (AbfA T-6) variant E294A, 3,4-dinitrophenyl α-L-arabinofuranoside |
| α-L-arabinofuranosidase (AbfA T-6)variant E294A | Geobacillus stearothermophilus | 3-nitrophenyl α-L-arabinofuranoside | 7 × 10⁻⁵ | — | — | pH 6, 40 °C | Open record: α-L-arabinofuranosidase (AbfA T-6) variant E294A, 3-nitrophenyl α-L-arabinofuranoside |
| α-L-arabinofuranosidase (AbfA T-6)variant E294A | Geobacillus stearothermophilus | 4-nitrophenyl α-L-arabinofuranoside | 5 × 10⁻⁶ | — | — | pH 6, 40 °C | Open record: α-L-arabinofuranosidase (AbfA T-6) variant E294A, 4-nitrophenyl α-L-arabinofuranoside |