Values CatLog read from this paper

Single amino acid substitution in Bacillus sphaericus phenylalanine dehydrogenase dramatically increases its discrimination between phenylalanine and tyrosine substrates

PMID 12234181doi:10.1021/bi020196aOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 86 records (248 values) from this paper: phenylalanine dehydrogenase (PheDH) from Bacillus sphaericus, Bacillus badius.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.4.1.20

Records read from this paper
EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsOpen record
phenylalanine dehydrogenase (PheDH)Bacillus badiusL-phenylalanine390.087448pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH), L-phenylalanine
phenylalanine dehydrogenase (PheDH)Bacillus badiusL-tyrosine348.63.9pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH), L-tyrosine
phenylalanine dehydrogenase (PheDH)Bacillus sphaericus2-oxohexanoate51133.9pH 8.6, 25 °COpen record: phenylalanine dehydrogenase (PheDH), 2-oxohexanoate
phenylalanine dehydrogenase (PheDH)Bacillus sphaericus2-oxopentanoate33520.63pH 8.6, 25 °COpen record: phenylalanine dehydrogenase (PheDH), 2-oxopentanoate
phenylalanine dehydrogenase (PheDH)Bacillus sphaericus3-(4-hydroxyphenyl)-2-oxopropanoate900.19474pH 8.6, 25 °COpen record: phenylalanine dehydrogenase (PheDH), 3-(4-hydroxyphenyl)-2-oxopropanoate
phenylalanine dehydrogenase (PheDH)Bacillus sphaericus3-methyl-2-oxobutanoate——0.55pH 8.6, 25 °COpen record: phenylalanine dehydrogenase (PheDH), 3-methyl-2-oxobutanoate
phenylalanine dehydrogenase (PheDH)Bacillus sphaericus3-phenyl-2-oxopropanoate1130.37305pH 8.6, 25 °COpen record: phenylalanine dehydrogenase (PheDH), 3-phenyl-2-oxopropanoate
phenylalanine dehydrogenase (PheDH)Bacillus sphaericus4-(methylsulfanyl)-2-oxobutanoate27113.7pH 8.6, 25 °COpen record: phenylalanine dehydrogenase (PheDH), 4-(methylsulfanyl)-2-oxobutanoate
phenylalanine dehydrogenase (PheDH)Bacillus sphaericus4-methyl-2-oxopentanoate27201.4pH 8.6, 25 °COpen record: phenylalanine dehydrogenase (PheDH), 4-methyl-2-oxopentanoate
phenylalanine dehydrogenase (PheDH)Bacillus sphaericusDL-3-methyl-2-oxopentanoate7.2130.55pH 8.6, 25 °COpen record: phenylalanine dehydrogenase (PheDH), DL-3-methyl-2-oxopentanoate
phenylalanine dehydrogenase (PheDH)Bacillus sphaericusL-isoleucine0.140.141pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH), L-isoleucine
phenylalanine dehydrogenase (PheDH)Bacillus sphaericusL-leucine0.70.551.3pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH), L-leucine
phenylalanine dehydrogenase (PheDH)Bacillus sphaericusL-methionine1.22.70.44pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH), L-methionine
phenylalanine dehydrogenase (PheDH)Bacillus sphaericusL-norleucine3.20.794pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH), L-norleucine
phenylalanine dehydrogenase (PheDH)Bacillus sphaericusL-norvaline1.43.30.42pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH), L-norvaline
phenylalanine dehydrogenase (PheDH)Bacillus sphaericusL-phenylalanine681.162pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH), L-phenylalanine
phenylalanine dehydrogenase (PheDH)Bacillus sphaericusL-tyrosine681.352pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH), L-tyrosine
phenylalanine dehydrogenase (PheDH)Bacillus sphaericusL-valine0.9120.46pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH), L-valine
phenylalanine dehydrogenase (PheDH)variant N145ABacillus sphaericus2-oxohexanoate660.25264pH 8.6, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145A, 2-oxohexanoate
phenylalanine dehydrogenase (PheDH)variant N145ABacillus sphaericus2-oxopentanoate541.439pH 8.6, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145A, 2-oxopentanoate
phenylalanine dehydrogenase (PheDH)variant N145ABacillus sphaericus3-(4-hydroxyphenyl)-2-oxopropanoate140.099141pH 8.6, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145A, 3-(4-hydroxyphenyl)-2-oxopropanoate
phenylalanine dehydrogenase (PheDH)variant N145ABacillus sphaericus3-methyl-2-oxobutanoate543.316pH 8.6, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145A, 3-methyl-2-oxobutanoate
phenylalanine dehydrogenase (PheDH)variant N145ABacillus sphaericus3-phenyl-2-oxopropanoate710.032367pH 8.6, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145A, 3-phenyl-2-oxopropanoate
phenylalanine dehydrogenase (PheDH)variant N145ABacillus sphaericus4-(methylsulfanyl)-2-oxobutanoate610.26235pH 8.6, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145A, 4-(methylsulfanyl)-2-oxobutanoate
phenylalanine dehydrogenase (PheDH)variant N145ABacillus sphaericus4-methyl-2-oxopentanoate240.22109pH 8.6, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145A, 4-methyl-2-oxopentanoate
phenylalanine dehydrogenase (PheDH)variant N145ABacillus sphaericusDL-3-methyl-2-oxopentanoate190.7426pH 8.6, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145A, DL-3-methyl-2-oxopentanoate
phenylalanine dehydrogenase (PheDH)variant N145ABacillus sphaericusL-isoleucine0.50.01729pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145A, L-isoleucine
phenylalanine dehydrogenase (PheDH)variant N145ABacillus sphaericusL-leucine0.980.03231pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145A, L-leucine
phenylalanine dehydrogenase (PheDH)variant N145ABacillus sphaericusL-methionine3.10.07840pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145A, L-methionine
phenylalanine dehydrogenase (PheDH)variant N145ABacillus sphaericusL-norleucine3.40.031110pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145A, L-norleucine
phenylalanine dehydrogenase (PheDH)variant N145ABacillus sphaericusL-norvaline1.80.1116pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145A, L-norvaline
phenylalanine dehydrogenase (PheDH)variant N145ABacillus sphaericusL-phenylalanine340.042809pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145A, L-phenylalanine
phenylalanine dehydrogenase (PheDH)variant N145ABacillus sphaericusL-tyrosine31215pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145A, L-tyrosine
phenylalanine dehydrogenase (PheDH)variant N145ABacillus sphaericusL-valine0.850.146.1pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145A, L-valine
phenylalanine dehydrogenase (PheDH)variant N145ABacillus sphaericusNAD+—0.21—pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145A, NAD+
phenylalanine dehydrogenase (PheDH)variant N145IBacillus sphaericus2-oxohexanoate610.25244pH 8.6, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145I, 2-oxohexanoate
phenylalanine dehydrogenase (PheDH)variant N145IBacillus sphaericus2-oxopentanoate612.623pH 8.6, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145I, 2-oxopentanoate
phenylalanine dehydrogenase (PheDH)variant N145IBacillus sphaericus3-(4-hydroxyphenyl)-2-oxopropanoate800.55145pH 8.6, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145I, 3-(4-hydroxyphenyl)-2-oxopropanoate
phenylalanine dehydrogenase (PheDH)variant N145IBacillus sphaericus3-methyl-2-oxobutanoate594.314pH 8.6, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145I, 3-methyl-2-oxobutanoate
phenylalanine dehydrogenase (PheDH)variant N145IBacillus sphaericus3-phenyl-2-oxopropanoate430.0123583pH 8.6, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145I, 3-phenyl-2-oxopropanoate
phenylalanine dehydrogenase (PheDH)variant N145IBacillus sphaericus4-(methylsulfanyl)-2-oxobutanoate470.43109pH 8.6, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145I, 4-(methylsulfanyl)-2-oxobutanoate
phenylalanine dehydrogenase (PheDH)variant N145IBacillus sphaericus4-methyl-2-oxopentanoate280.21133pH 8.6, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145I, 4-methyl-2-oxopentanoate
phenylalanine dehydrogenase (PheDH)variant N145IBacillus sphaericusDL-3-methyl-2-oxopentanoate16116pH 8.6, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145I, DL-3-methyl-2-oxopentanoate
phenylalanine dehydrogenase (PheDH)variant N145IBacillus sphaericusL-isoleucine0.190.01414pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145I, L-isoleucine
phenylalanine dehydrogenase (PheDH)variant N145IBacillus sphaericusL-leucine1.20.01580pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145I, L-leucine
phenylalanine dehydrogenase (PheDH)variant N145IBacillus sphaericusL-methionine3.10.1422pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145I, L-methionine
phenylalanine dehydrogenase (PheDH)variant N145IBacillus sphaericusL-norleucine5.40.052104pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145I, L-norleucine
phenylalanine dehydrogenase (PheDH)variant N145IBacillus sphaericusL-norvaline20.1414pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145I, L-norvaline
phenylalanine dehydrogenase (PheDH)variant N145IBacillus sphaericusL-phenylalanine130.014929pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145I, L-phenylalanine
phenylalanine dehydrogenase (PheDH)variant N145IBacillus sphaericusL-tyrosine211.316pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145I, L-tyrosine
phenylalanine dehydrogenase (PheDH)variant N145IBacillus sphaericusL-valine1.60.237pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145I, L-valine
phenylalanine dehydrogenase (PheDH)variant N145IBacillus sphaericusNAD+—0.19—pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145I, NAD+
phenylalanine dehydrogenase (PheDH)variant N145LBacillus sphaericus2-oxohexanoate420.22191pH 8.6, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145L, 2-oxohexanoate
phenylalanine dehydrogenase (PheDH)variant N145LBacillus sphaericus2-oxopentanoate402.914pH 8.6, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145L, 2-oxopentanoate
phenylalanine dehydrogenase (PheDH)variant N145LBacillus sphaericus3-(4-hydroxyphenyl)-2-oxopropanoate300.22136pH 8.6, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145L, 3-(4-hydroxyphenyl)-2-oxopropanoate
phenylalanine dehydrogenase (PheDH)variant N145LBacillus sphaericus3-methyl-2-oxobutanoate367.44.9pH 8.6, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145L, 3-methyl-2-oxobutanoate
phenylalanine dehydrogenase (PheDH)variant N145LBacillus sphaericus3-phenyl-2-oxopropanoate230.00952421pH 8.6, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145L, 3-phenyl-2-oxopropanoate
phenylalanine dehydrogenase (PheDH)variant N145LBacillus sphaericus4-(methylsulfanyl)-2-oxobutanoate250.2889pH 8.6, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145L, 4-(methylsulfanyl)-2-oxobutanoate
phenylalanine dehydrogenase (PheDH)variant N145LBacillus sphaericus4-methyl-2-oxopentanoate150.2268pH 8.6, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145L, 4-methyl-2-oxopentanoate
phenylalanine dehydrogenase (PheDH)variant N145LBacillus sphaericusDL-3-methyl-2-oxopentanoate131.68.1pH 8.6, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145L, DL-3-methyl-2-oxopentanoate
phenylalanine dehydrogenase (PheDH)variant N145LBacillus sphaericusL-isoleucine0.110.008213pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145L, L-isoleucine
phenylalanine dehydrogenase (PheDH)variant N145LBacillus sphaericusL-leucine0.450.02121pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145L, L-leucine
phenylalanine dehydrogenase (PheDH)variant N145LBacillus sphaericusL-methionine1.80.1512pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145L, L-methionine
phenylalanine dehydrogenase (PheDH)variant N145LBacillus sphaericusL-norleucine3.30.05659pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145L, L-norleucine
phenylalanine dehydrogenase (PheDH)variant N145LBacillus sphaericusL-norvaline0.910.118.3pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145L, L-norvaline
phenylalanine dehydrogenase (PheDH)variant N145LBacillus sphaericusL-phenylalanine8.50.032266pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145L, L-phenylalanine
phenylalanine dehydrogenase (PheDH)variant N145LBacillus sphaericusL-tyrosine9.40.6914pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145L, L-tyrosine
phenylalanine dehydrogenase (PheDH)variant N145LBacillus sphaericusL-valine0.620.272.3pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145L, L-valine
phenylalanine dehydrogenase (PheDH)variant N145LBacillus sphaericusNAD+—0.26—pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145L, NAD+
phenylalanine dehydrogenase (PheDH)variant N145VBacillus sphaericus2-oxohexanoate400.15267pH 8.6, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145V, 2-oxohexanoate
phenylalanine dehydrogenase (PheDH)variant N145VBacillus sphaericus2-oxopentanoate402.218pH 8.6, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145V, 2-oxopentanoate
phenylalanine dehydrogenase (PheDH)variant N145VBacillus sphaericus3-(4-hydroxyphenyl)-2-oxopropanoate880.61144pH 8.6, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145V, 3-(4-hydroxyphenyl)-2-oxopropanoate
phenylalanine dehydrogenase (PheDH)variant N145VBacillus sphaericus3-methyl-2-oxobutanoate464.211pH 8.6, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145V, 3-methyl-2-oxobutanoate
phenylalanine dehydrogenase (PheDH)variant N145VBacillus sphaericus3-phenyl-2-oxopropanoate600.0252.4 × 10³pH 8.6, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145V, 3-phenyl-2-oxopropanoate
phenylalanine dehydrogenase (PheDH)variant N145VBacillus sphaericus4-(methylsulfanyl)-2-oxobutanoate340.29117pH 8.6, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145V, 4-(methylsulfanyl)-2-oxobutanoate
phenylalanine dehydrogenase (PheDH)variant N145VBacillus sphaericus4-methyl-2-oxopentanoate180.2186pH 8.6, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145V, 4-methyl-2-oxopentanoate
phenylalanine dehydrogenase (PheDH)variant N145VBacillus sphaericusDL-3-methyl-2-oxopentanoate9.60.7613pH 8.6, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145V, DL-3-methyl-2-oxopentanoate
phenylalanine dehydrogenase (PheDH)variant N145VBacillus sphaericusL-isoleucine0.150.009516pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145V, L-isoleucine
phenylalanine dehydrogenase (PheDH)variant N145VBacillus sphaericusL-leucine0.840.01365pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145V, L-leucine
phenylalanine dehydrogenase (PheDH)variant N145VBacillus sphaericusL-methionine2.90.1322pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145V, L-methionine
phenylalanine dehydrogenase (PheDH)variant N145VBacillus sphaericusL-norleucine5.60.07476pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145V, L-norleucine
phenylalanine dehydrogenase (PheDH)variant N145VBacillus sphaericusL-norvaline1.90.1811pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145V, L-norvaline
phenylalanine dehydrogenase (PheDH)variant N145VBacillus sphaericusL-phenylalanine170.021810pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145V, L-phenylalanine
phenylalanine dehydrogenase (PheDH)variant N145VBacillus sphaericusL-tyrosine231.219pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145V, L-tyrosine
phenylalanine dehydrogenase (PheDH)variant N145VBacillus sphaericusL-valine1.40.275.2pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145V, L-valine
phenylalanine dehydrogenase (PheDH)variant N145VBacillus sphaericusNAD+—0.26—pH 10.4, 25 °COpen record: phenylalanine dehydrogenase (PheDH) variant N145V, NAD+