Values CatLog read from this paper
Role of tyrosine 238 in the active site of Rhodotorula gracilis D-amino acid oxidase. A site-directed mutagenesis study
PMID 12354107doi:10.1046/j.1432-1033.2002.t01-1-03173.xOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 25 records (27 values) from this paper: D-amino acid oxidase (RgDAAO) from Rhodotorula gracilis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 2 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| D-amino acid oxidase (RgDAAO) | Rhodotorula gracilis | Cephalosporin C | — | 5 | — | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO), Cephalosporin C |
| D-amino acid oxidase (RgDAAO) | Rhodotorula gracilis | D-Alanine | — | 0.8 | — | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO), D-Alanine |
| D-amino acid oxidase (RgDAAO) | Rhodotorula gracilis | D-Phenylalanine | — | 0.3 | — | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO), D-Phenylalanine |
| D-amino acid oxidase (RgDAAO) | Rhodotorula gracilis | D-Proline | — | 21.5 | — | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO), D-Proline |
| D-amino acid oxidase (RgDAAO) | Rhodotorula gracilis | D-Serine | — | 13.7 | — | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO), D-Serine |
| D-amino acid oxidase (RgDAAO) | Rhodotorula gracilis | D-Tryptophan | — | 0.3 | — | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO), D-Tryptophan |
| D-amino acid oxidase (RgDAAO) | Rhodotorula gracilis | D-Valine | — | 18.9 | — | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO), D-Valine |
| D-amino acid oxidase (RgDAAO)variant Y238F | Rhodotorula gracilis | Cephalosporin C | — | 1.9 | — | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant Y238F, Cephalosporin C |
| D-amino acid oxidase (RgDAAO)variant Y238F | Rhodotorula gracilis | D-alanine | 125 | 7.5 | 17 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant Y238F, D-alanine |
| D-amino acid oxidase (RgDAAO)variant Y238F | Rhodotorula gracilis | D-Alanine | — | 0.4 | — | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant Y238F, D-Alanine |
| D-amino acid oxidase (RgDAAO)variant Y238F | Rhodotorula gracilis | D-Phenylalanine | — | 0.04 | — | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant Y238F, D-Phenylalanine |
| D-amino acid oxidase (RgDAAO)variant Y238F | Rhodotorula gracilis | D-Proline | — | 13.5 | — | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant Y238F, D-Proline |
| D-amino acid oxidase (RgDAAO)variant Y238F | Rhodotorula gracilis | D-Serine | — | 1.7 | — | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant Y238F, D-Serine |
| D-amino acid oxidase (RgDAAO)variant Y238F | Rhodotorula gracilis | D-Tryptophan | — | 0.3 | — | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant Y238F, D-Tryptophan |
| D-amino acid oxidase (RgDAAO)variant Y238F | Rhodotorula gracilis | D-Valine | — | 6 | — | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant Y238F, D-Valine |
| D-amino acid oxidase (RgDAAO)variant Y238F | Rhodotorula gracilis | oxygen | — | 0.26 | — | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant Y238F, oxygen |
| D-amino acid oxidase (RgDAAO)variant Y238S | Rhodotorula gracilis | Cephalosporin C | — | 1.9 | — | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant Y238S, Cephalosporin C |
| D-amino acid oxidase (RgDAAO)variant Y238S | Rhodotorula gracilis | D-alanine | 120 | 7.8 | 15 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant Y238S, D-alanine |
| D-amino acid oxidase (RgDAAO)variant Y238S | Rhodotorula gracilis | D-Alanine | — | 0.4 | — | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant Y238S, D-Alanine |
| D-amino acid oxidase (RgDAAO)variant Y238S | Rhodotorula gracilis | D-Phenylalanine | — | 0.07 | — | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant Y238S, D-Phenylalanine |
| D-amino acid oxidase (RgDAAO)variant Y238S | Rhodotorula gracilis | D-Proline | — | 12.3 | — | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant Y238S, D-Proline |
| D-amino acid oxidase (RgDAAO)variant Y238S | Rhodotorula gracilis | D-Serine | — | 2.9 | — | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant Y238S, D-Serine |
| D-amino acid oxidase (RgDAAO)variant Y238S | Rhodotorula gracilis | D-Tryptophan | — | 0.2 | — | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant Y238S, D-Tryptophan |
| D-amino acid oxidase (RgDAAO)variant Y238S | Rhodotorula gracilis | D-Valine | — | 6.1 | — | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant Y238S, D-Valine |
| D-amino acid oxidase (RgDAAO)variant Y238S | Rhodotorula gracilis | oxygen | — | 0.96 | — | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant Y238S, oxygen |