Values CatLog read from this paper
Steady-state kinetics of the glutaminase reaction of CTP synthase from Lactococcus lactis. The role of the allosteric activator GTP incoupling between glutamine hydrolysis and CTP synthesis
PMID 12354108doi:10.1046/j.1432-1033.2002.03175.xOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 12 records (19 values) from this paper: CTP synthase from Lactococcus lactis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 7 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| CTP synthase | Lactococcus lactis | L-glutaminenucleotides: none; ITC assay; glutamine varied 0-4 mM | 0.084 | 1.1 | 0.076 calc. | pH 8, 30 °C | Open record: CTP synthase, L-glutamine |
| CTP synthase | Lactococcus lactis | L-glutaminenucleotides: 1 mM GTP; ITC assay; glutamine varied 0-4 mM | 0.259 | 1.15 | 0.225 calc. | pH 8, 30 °C | Open record: CTP synthase, L-glutamine |
| CTP synthase | Lactococcus lactis | L-glutaminenucleotides: 1 mM UTP; 1 mM ATP-γS; ITC assay; Table 1 | 0.082 | 1.26 | 0.065 calc. | pH 8, 30 °C | Open record: CTP synthase, L-glutamine |
| CTP synthase | Lactococcus lactis | L-glutaminenucleotides: 1 mM GTP; 1 mM UTP; 1 mM ATP-γS; ITC assay | 0.56 | 1 | 0.56 calc. | pH 8, 30 °C | Open record: CTP synthase, L-glutamine |
| CTP synthase | Lactococcus lactis | L-glutamineglutamine-dependent CTP synthesis; nucleotides: 1 mM UTP | 0.139 | 0.95 | 0.15 calc. | pH 8, 30 °C | Open record: CTP synthase, L-glutamine |
| CTP synthase | Lactococcus lactis | L-glutamineglutamine-dependent CTP synthesis; nucleotides: 0.1 mM GTP | 2.85 | 0.75 | 3.8 calc. | pH 8, 30 °C | Open record: CTP synthase, L-glutamine |
| CTP synthase | Lactococcus lactis | L-glutamineglutamine-dependent CTP synthesis; nucleotides: 1 mM GTP | 5.7 | 0.34 | 17 calc. | pH 8, 30 °C | Open record: CTP synthase, L-glutamine |
| CTP synthase | Lactococcus lactis | L-glutamine0.1 mM UTP; 0.1 mM ATP-γS; GTP varied; 15 mM glutamine | 1.2 | — | — | pH 8, 30 °C | Open record: CTP synthase, L-glutamine |
| CTP synthase | Lactococcus lactis | L-glutamine1 mM UTP; 1 mM ATP-γS; GTP varied; 15 mM glutamine; Table 2 | 1.054 | — | — | pH 8, 30 °C | Open record: CTP synthase, L-glutamine |
| CTP synthase | Lactococcus lactis | L-glutamineglutamine-dependent CTP synthesis; 0.1 mM UTP; 0.1 mM ATP | 0.195 | — | — | pH 8, 30 °C | Open record: CTP synthase, L-glutamine |
| CTP synthase | Lactococcus lactis | L-glutamineglutamine-dependent CTP synthesis; 0.1 mM UTP; 0.1 mM ATP | 0.028 | — | — | pH 8, 30 °C | Open record: CTP synthase, L-glutamine |
| CTP synthase | Lactococcus lactis | L-glutamineglutamine-dependent CTP synthesis; 1 mM UTP; 1 mM ATP | 6.4 | — | — | pH 8, 30 °C | Open record: CTP synthase, L-glutamine |