Values CatLog read from this paper
Essential features of the catalytic core of peptidyl-alpha-hydroxyglycine alpha-amidating lyase
PMID 12369828doi:10.1021/bi0260280Open in Browse
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CatLog read 7 records (8 values) from this paper: peptidyl-alpha-hydroxyglycine alpha-amidating lyase catalytic core (PALcc) from Rattus norvegicus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| peptidyl-alpha-hydroxyglycine alpha-amidating lyase catalytic core (PALcc) | Rattus norvegicus | N-alpha-acetyl-tyrosyl-valyl-alpha-hydroxyglycine | — | 0.0139 | — | pH 5.5, 37 °C | Open record: peptidyl-alpha-hydroxyglycine alpha-amidating lyase catalytic core (PALcc), N-alpha-acetyl-tyrosyl-valyl-alpha-hydroxyglycine |
| peptidyl-alpha-hydroxyglycine alpha-amidating lyase catalytic core (PALcc)variant D705N | Rattus norvegicus | N-alpha-acetyl-tyrosyl-valyl-alpha-hydroxyglycine | — | 0.0214 | — | pH 5.5, 37 °C | Open record: peptidyl-alpha-hydroxyglycine alpha-amidating lyase catalytic core (PALcc) variant D705N, N-alpha-acetyl-tyrosyl-valyl-alpha-hydroxyglycine |
| peptidyl-alpha-hydroxyglycine alpha-amidating lyase catalytic core (PALcc)variant E707Q | Rattus norvegicus | N-alpha-acetyl-tyrosyl-valyl-alpha-hydroxyglycine | — | 0.0102 | — | pH 5.5, 37 °C | Open record: peptidyl-alpha-hydroxyglycine alpha-amidating lyase catalytic core (PALcc) variant E707Q, N-alpha-acetyl-tyrosyl-valyl-alpha-hydroxyglycine |
| peptidyl-alpha-hydroxyglycine alpha-amidating lyase catalytic core (PALcc)variant H585A | Rattus norvegicus | N-alpha-acetyl-tyrosyl-valyl-alpha-hydroxyglycine | — | 0.0231 | — | pH 5.5, 37 °C | Open record: peptidyl-alpha-hydroxyglycine alpha-amidating lyase catalytic core (PALcc) variant H585A, N-alpha-acetyl-tyrosyl-valyl-alpha-hydroxyglycine |
| peptidyl-alpha-hydroxyglycine alpha-amidating lyase catalytic core (PALcc)variant H690A | Rattus norvegicus | N-alpha-acetyl-tyrosyl-valyl-alpha-hydroxyglycine | — | 0.0156 | — | pH 5.5, 37 °C | Open record: peptidyl-alpha-hydroxyglycine alpha-amidating lyase catalytic core (PALcc) variant H690A, N-alpha-acetyl-tyrosyl-valyl-alpha-hydroxyglycine |
| peptidyl-alpha-hydroxyglycine alpha-amidating lyase catalytic core (PALcc)variant H786A | Rattus norvegicus | N-alpha-acetyl-tyrosyl-valyl-alpha-hydroxyglycine | — | 0.0985 | — | pH 5.5, 37 °C | Open record: peptidyl-alpha-hydroxyglycine alpha-amidating lyase catalytic core (PALcc) variant H786A, N-alpha-acetyl-tyrosyl-valyl-alpha-hydroxyglycine |
| peptidyl-alpha-hydroxyglycine alpha-amidating lyase catalytic core (PALcc)variant Q551R (purified PALcc = PALcc-QR) | Rattus norvegicus | N-alpha-acetyl-tyrosyl-valyl-alpha-hydroxyglycine | 380 | 0.033 | 1.2 × 10⁴ calc. | pH 5.5, 37 °C | Open record: peptidyl-alpha-hydroxyglycine alpha-amidating lyase catalytic core (PALcc) variant Q551R (purified PALcc = PALcc-QR), N-alpha-acetyl-tyrosyl-valyl-alpha-hydroxyglycine |