CatLogenzyme kinetics catalog

Values CatLog read from this paper

Properties and regulation of the bifunctional enzyme HPr kinase/phosphatase in Bacillus subtilis

PMID 12411438doi:10.1074/jbc.m209052200Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 16 records (16 values) from this paper: HPr kinase/phosphatase (HPrK/P) from Bacillus subtilis.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
HPr kinase/phosphatase (HPrK/P)Bacillus subtilisadenosine 5'-triphosphateHPr fixed at 200 µM; no effector; cosubstrate HPr2.6——pH 8, 37 °COpen
HPr kinase/phosphatase (HPrK/P)Bacillus subtilisadenosine 5'-triphosphateHPr fixed at 200 µM; cosubstrate HPr1.8——pH 8, 37 °COpen
HPr kinase/phosphatase (HPrK/P)Bacillus subtilisadenosine 5'-triphosphateHPr fixed at 200 µM; cosubstrate HPr0.4——pH 8, 37 °COpen
HPr kinase/phosphatase (HPrK/P)Bacillus subtilisadenosine 5'-triphosphateCrh fixed at 169 µM; no effector; cosubstrate Crh0.8——pH 8, 37 °COpen
HPr kinase/phosphatase (HPrK/P)Bacillus subtilisadenosine 5'-triphosphateCrh fixed at 169 µM; cosubstrate Crh1.9——pH 8, 37 °COpen
HPr kinase/phosphatase (HPrK/P)Bacillus subtilisadenosine 5'-triphosphateCrh fixed at 169 µM; cosubstrate Crh1.5——pH 8, 37 °COpen
HPr kinase/phosphatase (HPrK/P)Bacillus subtilisCrh (catabolite repression HPr), His6-tagged5 mM MgCl2; 0.5 mM ATP; no effector0.9——pH 8, 37 °COpen
HPr kinase/phosphatase (HPrK/P)Bacillus subtilisCrh (catabolite repression HPr), His6-tagged7 mM MgCl2; 2 mM ATP; no effector2.3——pH 8, 37 °COpen
HPr kinase/phosphatase (HPrK/P)Bacillus subtilisCrh (catabolite repression HPr), His6-tagged7 mM MgCl2; 2 mM ATP; with 0.5 µM Pi (inorganic phosphate)0.7——pH 8, 37 °COpen
HPr kinase/phosphatase (HPrK/P)Bacillus subtilisCrh (catabolite repression HPr), His6-tagged7 mM MgCl2; 2 mM ATP; kcat from a Hill fit, n = 1.2 ± 0.23.7——pH 8, 37 °COpen
HPr kinase/phosphatase (HPrK/P)Bacillus subtilisCrh (catabolite repression HPr), His6-tagged7 mM MgCl2; 2 mM ATP; kcat from a Hill fit, n = 1.4 ± 0.17.5——pH 8, 37 °COpen
HPr kinase/phosphatase (HPrK/P)Bacillus subtilisHPr (histidine-containing protein), His6-tagged5 mM MgCl2; 0.5 mM ATP; no effector0.3——pH 8, 37 °COpen
HPr kinase/phosphatase (HPrK/P)Bacillus subtilisHPr (histidine-containing protein), His6-tagged7 mM MgCl2; 2 mM ATP; no effector0.6——pH 8, 37 °COpen
HPr kinase/phosphatase (HPrK/P)Bacillus subtilisHPr (histidine-containing protein), His6-tagged7 mM MgCl2; 2 mM ATP; with 0.5 µM Pi (inorganic phosphate)0.2——pH 8, 37 °COpen
HPr kinase/phosphatase (HPrK/P)Bacillus subtilisHPr (histidine-containing protein), His6-tagged7 mM MgCl2; 2 mM ATP; kcat from a Hill fit, n = 1.9 ± 0.31.9——pH 8, 37 °COpen
HPr kinase/phosphatase (HPrK/P)Bacillus subtilisHPr (histidine-containing protein), His6-tagged7 mM MgCl2; 2 mM ATP; kcat from a Hill fit, n = 1.5 ± 0.23——pH 8, 37 °COpen