Values CatLog read from this paper
Role of Thr(66) in porcine NADH-cytochrome b5 reductase in catalysis and control of the rate-limiting step in electron transfer
PMID 12459552doi:10.1074/jbc.m209838200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 11 records (22 values) from this paper: NADH-cytochrome b5 reductase from Sus scrofa (porcine liver).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 11 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| NADH-cytochrome b5 reductase | Sus scrofa (porcine liver) | potassium ferricyanide | 822 | 0.0025 | 3.3 × 10⁵ calc. | pH 7, 25 °C | Open record: NADH-cytochrome b5 reductase, potassium ferricyanide |
| NADH-cytochrome b5 reductase | Sus scrofa (porcine liver) | recombinant solubilized domain of porcine liver cytochrome b5 (residues Ala7-Lys93) | 911 | 0.0198 | 4.60 × 10⁴ calc. | pH 7, 25 °C | Open record: NADH-cytochrome b5 reductase, recombinant solubilized domain of porcine liver cytochrome b5 (residues Ala7-Lys93) |
| NADH-cytochrome b5 reductasevariant T66A | Sus scrofa (porcine liver) | NADH | 984 | 0.0029 | 3.4 × 10⁵ calc. | pH 7, 25 °C | Open record: NADH-cytochrome b5 reductase variant T66A, NADH |
| NADH-cytochrome b5 reductasevariant T66A | Sus scrofa (porcine liver) | potassium ferricyanide | 684 | 0.0022 | 3.1 × 10⁵ calc. | pH 7, 25 °C | Open record: NADH-cytochrome b5 reductase variant T66A, potassium ferricyanide |
| NADH-cytochrome b5 reductasevariant T66A | Sus scrofa (porcine liver) | recombinant solubilized domain of porcine liver cytochrome b5 (residues Ala7-Lys93) | 314 | 0.125 | 2.51 × 10³ calc. | pH 7, 25 °C | Open record: NADH-cytochrome b5 reductase variant T66A, recombinant solubilized domain of porcine liver cytochrome b5 (residues Ala7-Lys93) |
| NADH-cytochrome b5 reductasevariant T66S | Sus scrofa (porcine liver) | NADH | 1.15 × 10³ | 0.0028 | 4.1 × 10⁵ calc. | pH 7, 25 °C | Open record: NADH-cytochrome b5 reductase variant T66S, NADH |
| NADH-cytochrome b5 reductasevariant T66S | Sus scrofa (porcine liver) | potassium ferricyanide | 976 | 0.0031 | 3.1 × 10⁵ calc. | pH 7, 25 °C | Open record: NADH-cytochrome b5 reductase variant T66S, potassium ferricyanide |
| NADH-cytochrome b5 reductasevariant T66S | Sus scrofa (porcine liver) | recombinant solubilized domain of porcine liver cytochrome b5 (residues Ala7-Lys93) | 705 | 0.0133 | 5.30 × 10⁴ calc. | pH 7, 25 °C | Open record: NADH-cytochrome b5 reductase variant T66S, recombinant solubilized domain of porcine liver cytochrome b5 (residues Ala7-Lys93) |
| NADH-cytochrome b5 reductasevariant T66V | Sus scrofa (porcine liver) | NADH | 100 | 0.0028 | 3.6 × 10⁴ calc. | pH 7, 25 °C | Open record: NADH-cytochrome b5 reductase variant T66V, NADH |
| NADH-cytochrome b5 reductasevariant T66V | Sus scrofa (porcine liver) | potassium ferricyanide | 77 | 0.0016 | 4.8 × 10⁴ calc. | pH 7, 25 °C | Open record: NADH-cytochrome b5 reductase variant T66V, potassium ferricyanide |
| NADH-cytochrome b5 reductasevariant T66V | Sus scrofa (porcine liver) | recombinant solubilized domain of porcine liver cytochrome b5 (residues Ala7-Lys93) | 38 | 0.111 | 340 calc. | pH 7, 25 °C | Open record: NADH-cytochrome b5 reductase variant T66V, recombinant solubilized domain of porcine liver cytochrome b5 (residues Ala7-Lys93) |