Values CatLog read from this paper
Substrate specificity and enantioselectivity of 4-hydroxyacetophenone monooxygenase
PMID 12514023doi:10.1128/aem.69.1.419-426.2003Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 28 records (78 values) from this paper: 4-hydroxyacetophenone monooxygenase (HAPMO) from Pseudomonas fluorescens.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 6 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| 4-hydroxyacetophenone monooxygenase (HAPMO) | Pseudomonas fluorescens | 2,4-pentanedione | 2.1 | 4.9 | 0.43 | pH 7.5, 30 °C | Open record: 4-hydroxyacetophenone monooxygenase (HAPMO), 2,4-pentanedione |
| 4-hydroxyacetophenone monooxygenase (HAPMO) | Pseudomonas fluorescens | 2-acetylpyridine | 7.8 | 1.2 | 6.5 | pH 7.5, 30 °C | Open record: 4-hydroxyacetophenone monooxygenase (HAPMO), 2-acetylpyridine |
| 4-hydroxyacetophenone monooxygenase (HAPMO) | Pseudomonas fluorescens | 2-acetylpyrrole | 9.4 | 0.33 | 28 | pH 7.5, 30 °C | Open record: 4-hydroxyacetophenone monooxygenase (HAPMO), 2-acetylpyrrole |
| 4-hydroxyacetophenone monooxygenase (HAPMO) | Pseudomonas fluorescens | 2-hydroxyacetophenone | 6.7 | 0.61 | 20 | pH 7.5, 30 °C | Open record: 4-hydroxyacetophenone monooxygenase (HAPMO), 2-hydroxyacetophenone |
| 4-hydroxyacetophenone monooxygenase (HAPMO) | Pseudomonas fluorescens | 3-chloro-2-butanone | 7.4 | 23 | 0.32 | pH 7.5, 30 °C | Open record: 4-hydroxyacetophenone monooxygenase (HAPMO), 3-chloro-2-butanone |
| 4-hydroxyacetophenone monooxygenase (HAPMO) | Pseudomonas fluorescens | 3-hydroxyacetophenone | 4.8 | 1.4 | 3.4 | pH 7.5, 30 °C | Open record: 4-hydroxyacetophenone monooxygenase (HAPMO), 3-hydroxyacetophenone |
| 4-hydroxyacetophenone monooxygenase (HAPMO) | Pseudomonas fluorescens | 4-acetylpyridine | 0.5 | 1.9 | 0.3 | pH 7.5, 30 °C | Open record: 4-hydroxyacetophenone monooxygenase (HAPMO), 4-acetylpyridine |
| 4-hydroxyacetophenone monooxygenase (HAPMO) | Pseudomonas fluorescens | 4-aminoacetophenone | 12.7 | 8.2 × 10⁻⁴ | 1.5 × 10⁴ | pH 7.5, 30 °C | Open record: 4-hydroxyacetophenone monooxygenase (HAPMO), 4-aminoacetophenone |
| 4-hydroxyacetophenone monooxygenase (HAPMO) | Pseudomonas fluorescens | 4-aminoacetophenone | 11 | 8.7 × 10⁻⁴ | 1.3 × 10⁴ calc. | pH 7, 30 °C | Open record: 4-hydroxyacetophenone monooxygenase (HAPMO), 4-aminoacetophenone |
| 4-hydroxyacetophenone monooxygenase (HAPMO) | Pseudomonas fluorescens | 4-aminoacetophenone | 12.7 | 8.2 × 10⁻⁴ | 1.5 × 10⁴ calc. | pH 8, 30 °C | Open record: 4-hydroxyacetophenone monooxygenase (HAPMO), 4-aminoacetophenone |
| 4-hydroxyacetophenone monooxygenase (HAPMO) | Pseudomonas fluorescens | 4-aminoacetophenone | 11.1 | 9.9 × 10⁻⁴ | 1.1 × 10⁴ calc. | pH 9, 30 °C | Open record: 4-hydroxyacetophenone monooxygenase (HAPMO), 4-aminoacetophenone |
| 4-hydroxyacetophenone monooxygenase (HAPMO) | Pseudomonas fluorescens | 4-hydroxyacetophenone | 12.6 | 0.0092 | 1.4 × 10³ | pH 7.5, 30 °C | Open record: 4-hydroxyacetophenone monooxygenase (HAPMO), 4-hydroxyacetophenone |
| 4-hydroxyacetophenone monooxygenase (HAPMO) | Pseudomonas fluorescens | 4-hydroxyacetophenone | 10.4 | 0.0051 | 2.0 × 10³ calc. | pH 7, 30 °C | Open record: 4-hydroxyacetophenone monooxygenase (HAPMO), 4-hydroxyacetophenone |
| 4-hydroxyacetophenone monooxygenase (HAPMO) | Pseudomonas fluorescens | 4-hydroxyacetophenone | 12.8 | 0.012 | 1.1 × 10³ calc. | pH 8, 30 °C | Open record: 4-hydroxyacetophenone monooxygenase (HAPMO), 4-hydroxyacetophenone |
| 4-hydroxyacetophenone monooxygenase (HAPMO) | Pseudomonas fluorescens | 4-hydroxyacetophenone | 14.5 | 0.07 | 210 calc. | pH 9, 30 °C | Open record: 4-hydroxyacetophenone monooxygenase (HAPMO), 4-hydroxyacetophenone |
| 4-hydroxyacetophenone monooxygenase (HAPMO) | Pseudomonas fluorescens | 4-hydroxypropiophenone | 11.9 | 0.0024 | 5 × 10³ | pH 7.5, 30 °C | Open record: 4-hydroxyacetophenone monooxygenase (HAPMO), 4-hydroxypropiophenone |
| 4-hydroxyacetophenone monooxygenase (HAPMO) | Pseudomonas fluorescens | acetophenone | 13.2 | 2.3 | 5.7 | pH 7.5, 30 °C | Open record: 4-hydroxyacetophenone monooxygenase (HAPMO), acetophenone |
| 4-hydroxyacetophenone monooxygenase (HAPMO) | Pseudomonas fluorescens | acetylcyclohexane (cyclohexyl methyl ketone) | 3.9 | 4.8 | 0.81 | pH 7.5, 30 °C | Open record: 4-hydroxyacetophenone monooxygenase (HAPMO), acetylcyclohexane (cyclohexyl methyl ketone) |
| 4-hydroxyacetophenone monooxygenase (HAPMO) | Pseudomonas fluorescens | benzaldehyde | 2.2 | 1.6 | 1.4 | pH 7.5, 30 °C | Open record: 4-hydroxyacetophenone monooxygenase (HAPMO), benzaldehyde |
| 4-hydroxyacetophenone monooxygenase (HAPMO) | Pseudomonas fluorescens | butyrophenone | 1.2 | 2 | 0.6 | pH 7.5, 30 °C | Open record: 4-hydroxyacetophenone monooxygenase (HAPMO), butyrophenone |
| 4-hydroxyacetophenone monooxygenase (HAPMO) | Pseudomonas fluorescens | cyclohexanecarboxaldehyde | 5 | 3 | 1.7 | pH 7.5, 30 °C | Open record: 4-hydroxyacetophenone monooxygenase (HAPMO), cyclohexanecarboxaldehyde |
| 4-hydroxyacetophenone monooxygenase (HAPMO) | Pseudomonas fluorescens | hydroxyacetone | 4.1 | 29 | 0.14 | pH 7.5, 30 °C | Open record: 4-hydroxyacetophenone monooxygenase (HAPMO), hydroxyacetone |
| 4-hydroxyacetophenone monooxygenase (HAPMO) | Pseudomonas fluorescens | isobutyrophenone | 2.5 | 0.54 | 4.6 | pH 7.5, 30 °C | Open record: 4-hydroxyacetophenone monooxygenase (HAPMO), isobutyrophenone |
| 4-hydroxyacetophenone monooxygenase (HAPMO) | Pseudomonas fluorescens | methyl 4-tolyl sulfide (methyl p-tolyl sulfide) | 7.3 | 0.37 | 20 | pH 7.5, 30 °C | Open record: 4-hydroxyacetophenone monooxygenase (HAPMO), methyl 4-tolyl sulfide (methyl p-tolyl sulfide) |
| 4-hydroxyacetophenone monooxygenase (HAPMO) | Pseudomonas fluorescens | methyl phenyl sulfide (thioanisole) | 4.7 | 1.4 | 3.4 | pH 7.5, 30 °C | Open record: 4-hydroxyacetophenone monooxygenase (HAPMO), methyl phenyl sulfide (thioanisole) |
| 4-hydroxyacetophenone monooxygenase (HAPMO) | Pseudomonas fluorescens | propiophenone | 11 | 0.53 | 21 | pH 7.5, 30 °C | Open record: 4-hydroxyacetophenone monooxygenase (HAPMO), propiophenone |
| 4-hydroxyacetophenone monooxygenase (HAPMO) | Pseudomonas fluorescens | pyrrole-2-carboxaldehyde | 8.6 | 0.41 | 21 | pH 7.5, 30 °C | Open record: 4-hydroxyacetophenone monooxygenase (HAPMO), pyrrole-2-carboxaldehyde |
| 4-hydroxyacetophenone monooxygenase (HAPMO) | Pseudomonas fluorescens | rac-bicyclo[3.2.0]hept-2-en-6-one | 6.7 | 3.3 | 2 | pH 7.5, 30 °C | Open record: 4-hydroxyacetophenone monooxygenase (HAPMO), rac-bicyclo[3.2.0]hept-2-en-6-one |