CatLogenzyme kinetics catalog

Values CatLog read from this paper

Structures of shikimate dehydrogenase AroE and its Paralog YdiB. A common structural framework for different activities

PMID 12637497doi:10.1074/jbc.m300794200Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 10 records (20 values) from this paper: quinate/shikimate dehydrogenase YdiB, shikimate dehydrogenase AroE from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 10 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 1.1.1.25, EC 1.1.1.282

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
quinate/shikimate dehydrogenase YdiBEscherichia coliNAD+5 mM shikimic acid (fixed); cosubstrate shikimic acid0.050.0870.57 calc.pH 9, 20 °COpen
quinate/shikimate dehydrogenase YdiBEscherichia coliNAD+5 mM quinic acid (fixed); cosubstrate quinic acid0.050.1160.43 calc.pH 9, 20 °COpen
quinate/shikimate dehydrogenase YdiBEscherichia coliNADP+5 mM shikimic acid (fixed); cosubstrate shikimic acid0.120.11.2 calc.pH 9, 20 °COpen
quinate/shikimate dehydrogenase YdiBEscherichia coliNADP+5 mM quinic acid (fixed); cosubstrate quinic acid0.050.50.10 calc.pH 9, 20 °COpen
quinate/shikimate dehydrogenase YdiBEscherichia coliquinic acid5 mM NAD+ (fixed); cosubstrate NAD+0.050.0411.2 calc.pH 9, 20 °COpen
quinate/shikimate dehydrogenase YdiBEscherichia coliquinic acid5 mM NADP+ (fixed); cosubstrate NADP+0.050.5550.090 calc.pH 9, 20 °COpen
quinate/shikimate dehydrogenase YdiBEscherichia colishikimic acid5 mM NADP+ (fixed); cosubstrate NADP+0.120.120.97 calc.pH 9, 20 °COpen
quinate/shikimate dehydrogenase YdiBEscherichia colishikimic acid5 mM NAD+ (fixed); cosubstrate NAD+0.050.022.5 calc.pH 9, 20 °COpen
shikimate dehydrogenase AroEEscherichia coliNADP+2370.0564.2 × 10³ calc.pH 9, 20 °COpen
shikimate dehydrogenase AroEEscherichia colishikimic acid2370.0653.6 × 10³ calc.pH 9, 20 °COpen