Values CatLog read from this paper
The alpha-glucuronidase, GlcA67A, of Cellvibrio japonicus utilizes the carboxylate and methyl groups of aldobiouronic acid as important substrate recognition determinants
PMID 12654910doi:10.1074/jbc.m302205200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 10 records (24 values) from this paper: GlcA67A (alpha-glucuronidase, GH67) from Cellvibrio japonicus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| GlcA67A (alpha-glucuronidase, GH67) | Cellvibrio japonicus | 4-nitrophenyl 2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside | 143 | 0.21 | 667 | pH 7, 37 °C | Open record: GlcA67A (alpha-glucuronidase, GH67), 4-nitrophenyl 2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside |
| GlcA67A (alpha-glucuronidase, GH67)variant K288A | Cellvibrio japonicus | 4-nitrophenyl 2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside | 0.014 | 0.69 | 0.02 | pH 7, 37 °C | Open record: GlcA67A (alpha-glucuronidase, GH67) variant K288A, 4-nitrophenyl 2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside |
| GlcA67A (alpha-glucuronidase, GH67)variant K360A | Cellvibrio japonicus | 4-nitrophenyl 2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside | 0.2 | 1.6 | 0.13 | pH 7, 37 °C | Open record: GlcA67A (alpha-glucuronidase, GH67) variant K360A, 4-nitrophenyl 2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside |
| GlcA67A (alpha-glucuronidase, GH67)variant R325A | Cellvibrio japonicus | 4-nitrophenyl 2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside | — | — | 6.39 × 10⁻⁴ | pH 7, 37 °C | Open record: GlcA67A (alpha-glucuronidase, GH67) variant R325A, 4-nitrophenyl 2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside |
| GlcA67A (alpha-glucuronidase, GH67)variant V210A | Cellvibrio japonicus | 4-nitrophenyl 2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside | 220 | 0.86 | 256 | pH 7, 37 °C | Open record: GlcA67A (alpha-glucuronidase, GH67) variant V210A, 4-nitrophenyl 2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside |
| GlcA67A (alpha-glucuronidase, GH67)variant V210G | Cellvibrio japonicus | 4-nitrophenyl 2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside | 127 | 0.19 | 668 | pH 7, 37 °C | Open record: GlcA67A (alpha-glucuronidase, GH67) variant V210G, 4-nitrophenyl 2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside |
| GlcA67A (alpha-glucuronidase, GH67)variant V210N | Cellvibrio japonicus | 4-nitrophenyl 2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside | — | — | 63 | pH 7, 37 °C | Open record: GlcA67A (alpha-glucuronidase, GH67) variant V210N, 4-nitrophenyl 2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside |
| GlcA67A (alpha-glucuronidase, GH67)variant V210S | Cellvibrio japonicus | 4-nitrophenyl 2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside | — | — | 29 | pH 7, 37 °C | Open record: GlcA67A (alpha-glucuronidase, GH67) variant V210S, 4-nitrophenyl 2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside |
| GlcA67A (alpha-glucuronidase, GH67)variant W160A | Cellvibrio japonicus | 4-nitrophenyl 2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside | 0.09 | 2.1 | 0.043 | pH 7, 37 °C | Open record: GlcA67A (alpha-glucuronidase, GH67) variant W160A, 4-nitrophenyl 2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside |
| GlcA67A (alpha-glucuronidase, GH67)variant W543A | Cellvibrio japonicus | 4-nitrophenyl 2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside | 0.38 | 0.82 | 0.46 | pH 7, 37 °C | Open record: GlcA67A (alpha-glucuronidase, GH67) variant W543A, 4-nitrophenyl 2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside |