CatLogenzyme kinetics catalog

Values CatLog read from this paper

Kinetic and mechanistic characterization of recombinant Lactobacillus viridescens FemX (UDP-N-acetylmuramoyl pentapeptide-lysine N6-alanyltransferase)

PMID 12679335doi:10.1074/jbc.m301565200Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 15 records (28 values) from this paper: FemX (UDP-N-acetylmuramoyl pentapeptide-lysine N6-alanyltransferase) from Lactobacillus viridescens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 13 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 2.3.2.10

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
FemX (UDP-N-acetylmuramoyl pentapeptide-lysine N6-alanyltransferase)Lactobacillus viridescensalanyl-microhelix(Ala) (5'-GGGGCUAUAGCUCUAGCUCCACCA-3')UDP-MPP(Lys) (200 µM, saturating)10.830.029370 calc.pH 7.5, 25 °COpen
FemX (UDP-N-acetylmuramoyl pentapeptide-lysine N6-alanyltransferase)Lactobacillus viridescensalanyl-microhelix(Ala) (5'-GGGGCUAUAGCUCUAGCUCCACCA-3')UDP-MPP(Orn) (250 µM, saturating)10.30.041250 calc.pH 7.5, 25 °COpen
FemX (UDP-N-acetylmuramoyl pentapeptide-lysine N6-alanyltransferase)Lactobacillus viridescensalanyl-tRNA(Ala) (E. coli tRNA, in vitro transcript)UDP-MPP(Lys) (200 µM, saturating)110.015730 calc.pH 7.5, 25 °COpen
FemX (UDP-N-acetylmuramoyl pentapeptide-lysine N6-alanyltransferase)Lactobacillus viridescensalanyl-tRNA(Ala) (E. coli tRNA, in vitro transcript)UDP-MPP(Orn) (250 µM, saturating)10.330.031330 calc.pH 7.5, 25 °COpen
FemX (UDP-N-acetylmuramoyl pentapeptide-lysine N6-alanyltransferase)Lactobacillus viridescensalanyl-tRNA(Ser) (E. coli tRNA, in vitro transcript)4.330.018240 calc.pH 7.5, 25 °COpen
FemX (UDP-N-acetylmuramoyl pentapeptide-lysine N6-alanyltransferase)Lactobacillus viridescensseryl-tRNA(Ser) (E. coli tRNA, in vitro transcript)UDP-MPP(Lys) (200 µM, saturating)0.530.007571 calc.pH 7.5, 25 °COpen
FemX (UDP-N-acetylmuramoyl pentapeptide-lysine N6-alanyltransferase)Lactobacillus viridescensseryl-tRNA(Ser) (E. coli tRNA, in vitro transcript)UDP-MPP(Orn) (250 µM, saturating)0.40.01233 calc.pH 7.5, 25 °COpen
FemX (UDP-N-acetylmuramoyl pentapeptide-lysine N6-alanyltransferase)Lactobacillus viridescensUDP-N-acetylmuramoyl pentapeptide containing L-lysineAla-tRNAAla (75 µM, saturating)110.042260 calc.pH 7.5, 25 °COpen
FemX (UDP-N-acetylmuramoyl pentapeptide-lysine N6-alanyltransferase)Lactobacillus viridescensUDP-N-acetylmuramoyl pentapeptide containing L-lysineAla-tRNASer (100 µM, saturating)4.330.042100 calc.pH 7.5, 25 °COpen
FemX (UDP-N-acetylmuramoyl pentapeptide-lysine N6-alanyltransferase)Lactobacillus viridescensUDP-N-acetylmuramoyl pentapeptide containing L-lysineSer-tRNASer (75 µM, saturating)0.530.005597 calc.pH 7.5, 25 °COpen
FemX (UDP-N-acetylmuramoyl pentapeptide-lysine N6-alanyltransferase)Lactobacillus viridescensUDP-N-acetylmuramoyl pentapeptide containing L-ornithine10.330.1286 calc.pH 7.5, 25 °COpen
FemX (UDP-N-acetylmuramoyl pentapeptide-lysine N6-alanyltransferase)variant D109NLactobacillus viridescensalanyl-tRNA(Ala) (E. coli tRNA, in vitro transcript)0.180.0613.0 calc.pH 7.5, 25 °COpen
FemX (UDP-N-acetylmuramoyl pentapeptide-lysine N6-alanyltransferase)variant D109NLactobacillus viridescensUDP-N-acetylmuramoyl pentapeptide containing L-lysine—0.165—pH 7.5, 25 °COpen
FemX (UDP-N-acetylmuramoyl pentapeptide-lysine N6-alanyltransferase)variant E320QLactobacillus viridescensalanyl-tRNA(Ala) (E. coli tRNA, in vitro transcript)0.920.05417 calc.pH 7.5, 25 °COpen
FemX (UDP-N-acetylmuramoyl pentapeptide-lysine N6-alanyltransferase)variant E320QLactobacillus viridescensUDP-N-acetylmuramoyl pentapeptide containing L-lysine—0.067—pH 7.5, 25 °COpen