Values CatLog read from this paper

Identification of catalytic residues of Ca2+-independent 1,2-alpha-D-mannosidase from Aspergillus saitoi by site-directed mutagenesis

PMID 12702721doi:10.1074/jbc.m302621200Open in Browse

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CatLog read 9 records (27 values) from this paper: 1,2-alpha-D-mannosidase from Aspergillus saitoi.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.2.1.113

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
1,2-alpha-D-mannosidase	Aspergillus saitoi	methyl 2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranosideCa2+-free enzyme (0 mol Ca2+ bound per mol)	2.53	0.28	9.04	pH 5, 30 °C	Open record: 1,2-alpha-D-mannosidase, methyl 2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside
1,2-alpha-D-mannosidase	Aspergillus saitoi	methyl 2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside	1.95	0.29	6.84	pH 5, 30 °C	Open record: 1,2-alpha-D-mannosidase, methyl 2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside
1,2-alpha-D-mannosidasevariant D269E	Aspergillus saitoi	methyl 2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside	0.51	4.8	0.11	pH 5, 30 °C	Open record: 1,2-alpha-D-mannosidase variant D269E, methyl 2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside
1,2-alpha-D-mannosidasevariant E124D	Aspergillus saitoi	methyl 2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside	0.022	0.49	0.044	pH 5, 30 °C	Open record: 1,2-alpha-D-mannosidase variant E124D, methyl 2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside
1,2-alpha-D-mannosidasevariant E124Q	Aspergillus saitoi	methyl 2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside	0.0078	0.13	0.062	pH 5, 30 °C	Open record: 1,2-alpha-D-mannosidase variant E124Q, methyl 2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside
1,2-alpha-D-mannosidasevariant E273D	Aspergillus saitoi	methyl 2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside	0.37	7	0.054	pH 5, 30 °C	Open record: 1,2-alpha-D-mannosidase variant E273D, methyl 2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside
1,2-alpha-D-mannosidasevariant E411D	Aspergillus saitoi	methyl 2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside	1.4	8	0.18	pH 5, 30 °C	Open record: 1,2-alpha-D-mannosidase variant E411D, methyl 2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside
1,2-alpha-D-mannosidasevariant E414D	Aspergillus saitoi	methyl 2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside	0.037	8.6	0.0043	pH 5, 30 °C	Open record: 1,2-alpha-D-mannosidase variant E414D, methyl 2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside
1,2-alpha-D-mannosidasevariant E474D	Aspergillus saitoi	methyl 2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside	0.023	6.4	0.0036	pH 5, 30 °C	Open record: 1,2-alpha-D-mannosidase variant E474D, methyl 2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside