Values CatLog read from this paper
Enzyme I of the phosphotransferase system: induced-fit protonation of the reaction transition state by Cys-502
PMID 12705838doi:10.1021/bi034007fOpen in Browse
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CatLog read 17 records (37 values) from this paper: enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system (EI) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system (EI) | Escherichia coli | (E)-3-chlorophosphoenolpyruvate | 0.047 | 0.12 | 0.38 | pH 7.5, 30 °C | Open record: enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system (EI), (E)-3-chlorophosphoenolpyruvate |
| enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system (EI) | Escherichia coli | (Z)-3-chlorophosphoenolpyruvate[EI] = 3 µM (rate-limiting); 0-1 mM PEP analogue; Table 1 | 0.213 | 0.036 | 5.8 | pH 7.5, 30 °C | Open record: enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system (EI), (Z)-3-chlorophosphoenolpyruvate |
| enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system (EI) | Escherichia coli | (Z)-3-chlorophosphoenolpyruvate[EI] = 3 µM (rate-limiting); 0-1 mM PEP analogue; Table 1 | 0.263 | 0.025 | 10.5 | pH 7.5, 30 °C | Open record: enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system (EI), (Z)-3-chlorophosphoenolpyruvate |
| enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system (EI) | Escherichia coli | (Z)-3-chlorophosphoenolpyruvate[EI] = 1.3 µM; Table 4 | 0.13 | — | — | pH 7.5, 30 °C | Open record: enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system (EI), (Z)-3-chlorophosphoenolpyruvate |
| enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system (EI) | Escherichia coli | (Z)-3-fluorophosphoenolpyruvate[EI] = 0.006 µM (rate-limiting); 0-1 mM PEP analogue | 6.2 | 0.11 | 57 | pH 7.5, 30 °C | Open record: enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system (EI), (Z)-3-fluorophosphoenolpyruvate |
| enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system (EI) | Escherichia coli | (Z)-3-fluorophosphoenolpyruvate[EI] = 0.013 µM | 6.7 | — | — | pH 7.5, 30 °C | Open record: enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system (EI), (Z)-3-fluorophosphoenolpyruvate |
| enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system (EI) | Escherichia coli | (Z)-3-methylphosphoenolpyruvate ((Z)-phosphoenolbutyrate)[EI] = 0.024 µM (rate-limiting); 0-1 mM PEP analogue | 4.75 | 0.43 | 11 | pH 7.5, 30 °C | Open record: enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system (EI), (Z)-3-methylphosphoenolpyruvate ((Z)-phosphoenolbutyrate) |
| enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system (EI) | Escherichia coli | (Z)-3-methylphosphoenolpyruvate ((Z)-phosphoenolbutyrate)[EI] = 0.032 µM | 6.5 | — | — | pH 7.5, 30 °C | Open record: enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system (EI), (Z)-3-methylphosphoenolpyruvate ((Z)-phosphoenolbutyrate) |
| enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system (EI) | Escherichia coli | phosphoenolpyruvate[EI] = 0.005 µM (rate-limiting); 0-1 mM PEP analogue | 63.8 | 0.14 | 450 | pH 7.5, 30 °C | Open record: enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system (EI), phosphoenolpyruvate |
| enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system (EI) | Escherichia coli | phosphoenolpyruvate[EI] = 0.005 µM | 63 | — | — | pH 7.5, 30 °C | Open record: enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system (EI), phosphoenolpyruvate |
| enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system (EI) | Escherichia coli | phosphoenolpyruvateEI dimeric at total EI concentration above 20 nM | 94.2 | — | — | pH 7.5, 30 °C | Open record: enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system (EI), phosphoenolpyruvate |
| enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system (EI)variant C502A | Escherichia coli | (E)-3-chlorophosphoenolpyruvate | 0.009 | 0.13 | 0.07 | pH 7.5, 30 °C | Open record: enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system (EI) variant C502A, (E)-3-chlorophosphoenolpyruvate |
| enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system (EI)variant C502A | Escherichia coli | (Z)-3-chlorophosphoenolpyruvate[EI] = 0.8 µM (rate-limiting); 0-1 mM PEP analogue | 0.18 | 0.054 | 3.3 | pH 7.5, 30 °C | Open record: enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system (EI) variant C502A, (Z)-3-chlorophosphoenolpyruvate |
| enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system (EI)variant C502A | Escherichia coli | (Z)-3-chlorophosphoenolpyruvate[EI] = 1.5 µM | 0.12 | — | — | pH 7.5, 30 °C | Open record: enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system (EI) variant C502A, (Z)-3-chlorophosphoenolpyruvate |
| enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system (EI)variant C502A | Escherichia coli | (Z)-3-fluorophosphoenolpyruvate[EI] = 5 µM (rate-limiting); 0-1 mM PEP analogue | 0.025 | 0.035 | 0.72 | pH 7.5, 30 °C | Open record: enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system (EI) variant C502A, (Z)-3-fluorophosphoenolpyruvate |
| enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system (EI)variant C502A | Escherichia coli | (Z)-3-fluorophosphoenolpyruvate[EI] = 5 µM | 0.025 | — | — | pH 7.5, 30 °C | Open record: enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system (EI) variant C502A, (Z)-3-fluorophosphoenolpyruvate |
| enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system (EI)variant C502A | Escherichia coli | phosphoenolpyruvate | 0.0058 | 0.041 | 0.13 | pH 7.5, 30 °C | Open record: enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system (EI) variant C502A, phosphoenolpyruvate |