Values CatLog read from this paper
Analysis of the importance of the metallo-beta-lactamase active site loop in substrate binding and catalysis
PMID 12725860doi:10.1016/s1074-5521(03)00070-xOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 36 records (98 values) from this paper: BcII metallo-β-lactamase, IMP-1 metallo-β-lactamase from Bacillus cereus, Pseudomonas aeruginosa.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| BcII metallo-β-lactamase | Bacillus cereus | Benzylpenicillin | 190 | 0.15 | 1.2 × 10³ | pH 7.5, 30 °C | Open record: BcII metallo-β-lactamase, Benzylpenicillin |
| BcII metallo-β-lactamase | Bacillus cereus | Cefotaxime | 120 | 0.024 | 5 × 10³ | pH 7.5, 30 °C | Open record: BcII metallo-β-lactamase, Cefotaxime |
| BcII metallo-β-lactamase | Bacillus cereus | Cefoxitin | 0.34 | 0.24 | 1.4 | pH 7.5, 30 °C | Open record: BcII metallo-β-lactamase, Cefoxitin |
| BcII metallo-β-lactamase | Bacillus cereus | cephaloridine | — | — | 300 | pH 7.5, 30 °C | Open record: BcII metallo-β-lactamase, cephaloridine |
| BcII metallo-β-lactamase | Bacillus cereus | Imipenem | 117 | 0.09 | 1.3 × 10³ | pH 7.5, 30 °C | Open record: BcII metallo-β-lactamase, Imipenem |
| BcII metallo-β-lactamase | Bacillus cereus | Nitrocefin | 27 | 0.01 | 2.7 × 10³ | pH 7.5, 30 °C | Open record: BcII metallo-β-lactamase, Nitrocefin |
| BcII metallo-β-lactamasevariant BcIIΔIMP (BcII loop 60SFNGEA66 replaced by IMP-1 loop EVNGWGV) | Bacillus cereus | Benzylpenicillin | 293 | 0.032 | 9.2 × 10³ | pH 7.5, 30 °C | Open record: BcII metallo-β-lactamase variant BcIIΔIMP (BcII loop 60SFNGEA66 replaced by IMP-1 loop EVNGWGV), Benzylpenicillin |
| BcII metallo-β-lactamasevariant BcIIΔIMP (BcII loop 60SFNGEA66 replaced by IMP-1 loop EVNGWGV) | Bacillus cereus | Cefotaxime | 32 | 0.007 | 4.6 × 10³ | pH 7.5, 30 °C | Open record: BcII metallo-β-lactamase variant BcIIΔIMP (BcII loop 60SFNGEA66 replaced by IMP-1 loop EVNGWGV), Cefotaxime |
| BcII metallo-β-lactamasevariant BcIIΔIMP (BcII loop 60SFNGEA66 replaced by IMP-1 loop EVNGWGV) | Bacillus cereus | Cefoxitin | 0.35 | 0.063 | 5.5 | pH 7.5, 30 °C | Open record: BcII metallo-β-lactamase variant BcIIΔIMP (BcII loop 60SFNGEA66 replaced by IMP-1 loop EVNGWGV), Cefoxitin |
| BcII metallo-β-lactamasevariant BcIIΔIMP (BcII loop 60SFNGEA66 replaced by IMP-1 loop EVNGWGV) | Bacillus cereus | cephaloridine | 156 | 0.047 | 3.3 × 10³ | pH 7.5, 30 °C | Open record: BcII metallo-β-lactamase variant BcIIΔIMP (BcII loop 60SFNGEA66 replaced by IMP-1 loop EVNGWGV), cephaloridine |
| BcII metallo-β-lactamasevariant BcIIΔIMP (BcII loop 60SFNGEA66 replaced by IMP-1 loop EVNGWGV) | Bacillus cereus | Imipenem | 104 | 0.078 | 1.3 × 10³ | pH 7.5, 30 °C | Open record: BcII metallo-β-lactamase variant BcIIΔIMP (BcII loop 60SFNGEA66 replaced by IMP-1 loop EVNGWGV), Imipenem |
| BcII metallo-β-lactamasevariant BcIIΔIMP (BcII loop 60SFNGEA66 replaced by IMP-1 loop EVNGWGV) | Bacillus cereus | Nitrocefin | 68 | 0.0052 | 1.3 × 10⁴ | pH 7.5, 30 °C | Open record: BcII metallo-β-lactamase variant BcIIΔIMP (BcII loop 60SFNGEA66 replaced by IMP-1 loop EVNGWGV), Nitrocefin |
| BcII metallo-β-lactamasevariant BcIIΔIMP W64A (IMP-1 loop in BcII, then W64A) | Bacillus cereus | Benzylpenicillin | 143 | 0.154 | 900 | pH 7.5, 30 °C | Open record: BcII metallo-β-lactamase variant BcIIΔIMP W64A (IMP-1 loop in BcII, then W64A), Benzylpenicillin |
| BcII metallo-β-lactamasevariant BcIIΔIMP W64A (IMP-1 loop in BcII, then W64A) | Bacillus cereus | Cefotaxime | 27 | 0.038 | 700 | pH 7.5, 30 °C | Open record: BcII metallo-β-lactamase variant BcIIΔIMP W64A (IMP-1 loop in BcII, then W64A), Cefotaxime |
| BcII metallo-β-lactamasevariant BcIIΔIMP W64A (IMP-1 loop in BcII, then W64A) | Bacillus cereus | Cefoxitin | 0.66 | 0.93 | 0.7 | pH 7.5, 30 °C | Open record: BcII metallo-β-lactamase variant BcIIΔIMP W64A (IMP-1 loop in BcII, then W64A), Cefoxitin |
| BcII metallo-β-lactamasevariant BcIIΔIMP W64A (IMP-1 loop in BcII, then W64A) | Bacillus cereus | cephaloridine | 57 | 0.56 | 100 | pH 7.5, 30 °C | Open record: BcII metallo-β-lactamase variant BcIIΔIMP W64A (IMP-1 loop in BcII, then W64A), cephaloridine |
| BcII metallo-β-lactamasevariant BcIIΔIMP W64A (IMP-1 loop in BcII, then W64A) | Bacillus cereus | Imipenem | 180 | 0.9 | 200 | pH 7.5, 30 °C | Open record: BcII metallo-β-lactamase variant BcIIΔIMP W64A (IMP-1 loop in BcII, then W64A), Imipenem |
| BcII metallo-β-lactamasevariant BcIIΔIMP W64A (IMP-1 loop in BcII, then W64A) | Bacillus cereus | Nitrocefin | 3.2 | 0.011 | 300 | pH 7.5, 30 °C | Open record: BcII metallo-β-lactamase variant BcIIΔIMP W64A (IMP-1 loop in BcII, then W64A), Nitrocefin |
| IMP-1 metallo-β-lactamase | Pseudomonas aeruginosa | Benzylpenicillin | 200 | 0.32 | 630 | pH 7.5, 30 °C | Open record: IMP-1 metallo-β-lactamase, Benzylpenicillin |
| IMP-1 metallo-β-lactamase | Pseudomonas aeruginosa | Cefotaxime | 13.4 | 0.0028 | 4.8 × 10³ | pH 7.5, 30 °C | Open record: IMP-1 metallo-β-lactamase, Cefotaxime |
| IMP-1 metallo-β-lactamase | Pseudomonas aeruginosa | Cefoxitin | 9 | 0.003 | 3 × 10³ | pH 7.5, 30 °C | Open record: IMP-1 metallo-β-lactamase, Cefoxitin |
| IMP-1 metallo-β-lactamase | Pseudomonas aeruginosa | cephaloridine | 32 | 0.005 | 6.4 × 10³ | pH 7.5, 30 °C | Open record: IMP-1 metallo-β-lactamase, cephaloridine |
| IMP-1 metallo-β-lactamase | Pseudomonas aeruginosa | Imipenem | 68 | 0.025 | 2.7 × 10³ | pH 7.5, 30 °C | Open record: IMP-1 metallo-β-lactamase, Imipenem |
| IMP-1 metallo-β-lactamase | Pseudomonas aeruginosa | Nitrocefin | 130 | 0.0082 | 1.4 × 10⁴ | pH 7.5, 30 °C | Open record: IMP-1 metallo-β-lactamase, Nitrocefin |
| IMP-1 metallo-β-lactamasevariant W64A | Pseudomonas aeruginosa | Benzylpenicillin | 86 | 1.25 | 70 | pH 7.5, 30 °C | Open record: IMP-1 metallo-β-lactamase variant W64A, Benzylpenicillin |
| IMP-1 metallo-β-lactamasevariant W64A | Pseudomonas aeruginosa | Cefotaxime | 98 | 0.061 | 1.5 × 10³ | pH 7.5, 30 °C | Open record: IMP-1 metallo-β-lactamase variant W64A, Cefotaxime |
| IMP-1 metallo-β-lactamasevariant W64A | Pseudomonas aeruginosa | Cefoxitin | 40 | 0.045 | 900 | pH 7.5, 30 °C | Open record: IMP-1 metallo-β-lactamase variant W64A, Cefoxitin |
| IMP-1 metallo-β-lactamasevariant W64A | Pseudomonas aeruginosa | cephaloridine | — | — | 310 | pH 7.5, 30 °C | Open record: IMP-1 metallo-β-lactamase variant W64A, cephaloridine |
| IMP-1 metallo-β-lactamasevariant W64A | Pseudomonas aeruginosa | Imipenem | 67 | 0.12 | 550 | pH 7.5, 30 °C | Open record: IMP-1 metallo-β-lactamase variant W64A, Imipenem |
| IMP-1 metallo-β-lactamasevariant W64A | Pseudomonas aeruginosa | Nitrocefin | 700 | 0.16 | 4.3 × 10³ | pH 7.5, 30 °C | Open record: IMP-1 metallo-β-lactamase variant W64A, Nitrocefin |
| IMP-1 metallo-β-lactamaseΔ61–66 (deletion of loop residues 61VNGWGV66) | Pseudomonas aeruginosa | Benzylpenicillin | — | — | 1.7 | pH 7.5, 30 °C | Open record: IMP-1 metallo-β-lactamase Δ61–66 (deletion of loop residues 61VNGWGV66), Benzylpenicillin |
| IMP-1 metallo-β-lactamaseΔ61–66 (deletion of loop residues 61VNGWGV66) | Pseudomonas aeruginosa | Cefotaxime | 129 | 0.45 | 280 | pH 7.5, 30 °C | Open record: IMP-1 metallo-β-lactamase Δ61–66 (deletion of loop residues 61VNGWGV66), Cefotaxime |
| IMP-1 metallo-β-lactamaseΔ61–66 (deletion of loop residues 61VNGWGV66) | Pseudomonas aeruginosa | Cefoxitin | 8.6 | 0.097 | 90 | pH 7.5, 30 °C | Open record: IMP-1 metallo-β-lactamase Δ61–66 (deletion of loop residues 61VNGWGV66), Cefoxitin |
| IMP-1 metallo-β-lactamaseΔ61–66 (deletion of loop residues 61VNGWGV66) | Pseudomonas aeruginosa | cephaloridine | — | — | 15 | pH 7.5, 30 °C | Open record: IMP-1 metallo-β-lactamase Δ61–66 (deletion of loop residues 61VNGWGV66), cephaloridine |
| IMP-1 metallo-β-lactamaseΔ61–66 (deletion of loop residues 61VNGWGV66) | Pseudomonas aeruginosa | Imipenem | 100 | 0.09 | 1.1 × 10³ | pH 7.5, 30 °C | Open record: IMP-1 metallo-β-lactamase Δ61–66 (deletion of loop residues 61VNGWGV66), Imipenem |
| IMP-1 metallo-β-lactamaseΔ61–66 (deletion of loop residues 61VNGWGV66) | Pseudomonas aeruginosa | Nitrocefin | — | — | 2.7 × 10³ | pH 7.5, 30 °C | Open record: IMP-1 metallo-β-lactamase Δ61–66 (deletion of loop residues 61VNGWGV66), Nitrocefin |